Investigating the effect of high-intensity interval training and moderate-intensity continues training on the protein content of PI3K, AKT, and FOXO3 in the heart of rats fed a high-fat diet

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Document Type : Research Paper I Open Access I Released under (CC BY-NC 4.0) license

Authors

1 Associate Professor, Department of Exercise Physiology, Faculty of Educational Sciences and Psychology, University of Mohaghegh Ardabili, Ardabil, Iran

2 Postdoctoral Researcher, Department of Exercise Physiology, Faculty of Educational Sciences and Psychology, University of Mohaghegh Ardabili, Ardabil, Iran

3 1. Associate Professor, Department of Physical Education and Sports Science, Gachsaran branch, Islamic Azad University, Gachsaran, Iran

Abstract

Aim: Although lack of exercise activity, and a high-fat diet (HFD) cause an imbalance between energy consumption and intake, exercise training prevents and controls obesity. Therefore, the recent study aimed  to compare the effect of high-intensity interval training (HIIT) and moderate-intensity continues training (MICT) on the protein content of PI3K, AKT, and FOXO3 in the heart of rats fed a high-fat diet. Methods: In this experimental study, 20 male Wistar rats (approximate age=five weeks and average weight 110±10) were purchased and randomly placed in four groups of five: healthy control with normal diet (HC), sedentary control with HFD (SC), HIIT with HFD (HIIT), and MICT with HFD (MICT). Eight weeks of HIIT and MICT was performed for two groups, separately.  To induce obesity, the animals were fed a high-fat diet for eight weeks. Western blot method was used to check protein content of PI3K, AKT, and FOXO3. One-way analysis of variance with Tukey's post-hoc test was used to analyze the data (with a significance level of P≤0.05). Results: After eight weeks of MICT and HIIT, blood glucose, insulin, body weight, triglycerides (TG), total cholesterol (TC), low-density lipoprotein-cholesterol (LDL-C), inflammatory factors, and FOXO3 protein content significantly decreased (P=0/001), and protein content of AKT as well as PI3K significantly increased (P=0/001) in HIIT and MICT groups compared to the SC group. Conclusion: It seems that both types of exercise training protocols played an important role in controlling factors and pathways related to heart failure by modulating insulin levels, blood glucose, lipid profile, inflammatory factors, as well as increase in protein content of PI3K and AKT and decrease in  protein content of FOXO3 in HFD-fed rats.
 

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References

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Journal of Applied Health Studies in Sport Physiology

Articles in Press, Accepted Manuscript
Available Online from 21 January 2025

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History

  • Receive Date: 21 October 2024
  • Revise Date: 16 December 2024
  • Accept Date: 21 January 2025

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