The effect of high-intensity interval training and high-protein diet on the levels of UCP-2 and MCP-1 and insulin sensitivity in visceral fat and skeletal muscle of obese male rats

Articles in Press

Document Type : Research Paper I Open Access I Released under (CC BY-NC 4.0) license

Authors

1 Department of Sport Sciences, Faculty of Sport Sciences, University of Birjand, Birjand, Iran

2 Cellular and Molecular Endocrine Research Center, Research Institute for Endocrine Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Abstract

Aims: Exercise training and diet can prevent obesity, inflammation, and insulin resistance. Present study aimed to investigate the effect of 10 weeks of high-intensity interval training (HIIT) and high-protein diet (HPD) on the levels of UCP-2 and MCP-1 in visceral fat tissue and gastrocnemius muscle, plasma levels of glucose and insulin, and insulin sensitivity. Methods: Forty obese male Wistar rats (weight 386.46±31.79 g) into five group included: HIIT, HPD, HIIT+ HPD, obese control-1 (OC-1: having high-fat diet during intervention), and obese control-2 (OC-2: cutting off high-fat diet during intervention and continuing normal diet) and eight non-obese rats (NC: non-obese control group, weight 283.10±42.26 g) were studied. Training protocol included running five days a week on the treadmill for 10 weeks with at intensity of 90% of maximum oxygen uptake. Forty-eight hours after the last intervention session, blood and tissue samples were taken to measure biochemical variables. Data were analyzed using one-way analysis of variance and Kruskal–Wallis tests at a significance level of P≤0.05. Results: No significant difference was observed in the tissue levels of UCP-2 and MCP-1 and plasma levels of insulin among groups. Plasma glucose levels in the OC-1 group was significantly higher than in the NC, OC-2, HIIT, HPD, and HIIT+HPD groups, it was significantly higher in the OC-2, HIIT, HPD and HIIT+HPD groups than the NC group, and it was significantly higher in the HPD group than in the OC-2 group (P≤0.05). Also, insulin sensitivity index was significantly higher in the OC-2 group than in the OC-1, HIIT and HPD groups (P≤0.05). Conclusion: Long periods of using a high-fat diet, although it increases the risk of obesity, may not change the inflammatory state. On the other hand, modifying the diet and doing regular exercise can lead to the improvement of glycemic disorder caused by consuming a high-fat

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Journal of Applied Health Studies in Sport Physiology

Articles in Press, Accepted Manuscript
Available Online from 16 November 2024

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History

  • Receive Date: 17 July 2024
  • Revise Date: 16 November 2024
  • Accept Date: 16 November 2024

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