Document Type : Research Paper I Open Access I Released under (CC BY-NC 4.0) license
Authors
1
PhD Student of Exercise Physiology, Department of Physical Education and Sport Science, Islamic Azad University of Tabriz, Iran.
2
Tabriz Branch, Islamic Azad University
3
2. Assist Prof, Department of Physical Education, Tabriz branch, Islamic Azad university, Tabriz, Iran
4
Assistant Professor, Department of Physical Education and Sports Science, Faculty of Social Sciences, Imam Khomeini International University, Qazvin, Iran.
5
Department of Veterinary Medicine, Tabriz Azad University of Medical Sciences, Iran.
Abstract
Purpose: This research aims to investigate the effect of intense interval exercise training and melatonin supplementation on oxidative stress markers and antioxidant enzyme activity, as well as creatinine and blood urea nitrogen concentrations, as well as histopathological changes to evaluate kidney function in male rats with ischemia renal reperfusion was performed. Methods: 30 male Wistar rats were randomly divided into 5 groups, healthy, Ischemia/Reperfusion (IR), IR + intense interval training, IR + melatonin supplement and IR + high intensive interval training + melatonin supplement. Except for the healthy group, the animals were subjected to ischemia-reperfusion in other test groups. The high intensive interval training protocol was implemented for 12 weeks (5 days per week). Oxidative indicators of SOD, GPX and MDA were measured by spectrophotometric method and for BUN and Cr indicators, urea was quantitatively detected by (Urease-GLDH) method and creatinine by (JAFFE) method in plasma serum sample by photometric method with Pars test kit. Results: Because of the normal distribution of parameters, the data were analysed by analysis of variance, Fisher's test, and the comparison test of two population at the significance level of p<0.05. A decrease in kidney function after IR was observed with a significant increase in Cr, BUN and MDA levels and a decrease in GPX and SOD. The therapeutic effect of melatonin led to significant decrease in BUN, Cr and MDA and an increase in the activity of SOD, GPX compared to the ischemic group. Exercise training caused a significant decrease in BUN and an increase in GPX compared to the ischemic group and a significant increase in MDA and a decrease in SOD compared to the healthy group. Conclusions: It seems that the high-intensity interval training and melatonin supplementation are effective in protecting kidney tissue against apoptotic damage and inflammation caused by renal ischemia.
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