Document Type : Research Paper I Open Access I Released under (CC BY-NC 4.0) license
Authors
1
Department of Sport Physiology, Faculty of Educational Sciences and Psychology, University of Mohaghegh Ardabili, Ardabil, Iran
2
Department of Exercise Physiology, Faculty of Educational Sciences and Psychology, University of Mohaghegh Ardabili, Ardabil, Iran
3
Department of Sport Physiology, Faculty of Education and Psychology, University of Mohaghegh Ardabili, Ardabil, Iran
Abstract
Purpose: The purpose of this study was to investigate the effect of two months of intense intermittent exercise with sodium citrate supplementation on intracellular redox status and oxidative stress indices in diabetic rats.
Methodology: In this quasi-experimental and fundamental study, 50 male Wistar rats were randomly divided into 5 groups: healthy control, diabetic control, diabetic - training, diabetic - sodium citrate supplementation and diabetic - training sodium citrate supplementation. The training protocol consisted of 8 weeks HIIT training at 90% of the maximum speed. Also, the supplemental and exercise-supplement groups received sodium citrate supplement in a water-soluble form three hours before exercise (764 mg/kg). 72 hours after the last training session of abdominal ajof vein blood sampling to study the variables of the study by ELISA method.To compare the study groups, a one-way analysis of variance and LSD post hoc test were used in SPSS software (p≤0.05).
Results: The results showed that the levels of GSH, GSSG, GSH/GSSG and SOD were significantly different between the control-healthy, control-diabetic, diabetic-exercise, diabetic-supplement and diabetic-supplement-exercise groups (P≤0.05). Also, the results of post hoc test (LSD) showed that diabetic-supplement and diabetic-supplement-exercise led to a significant increase in GSH, SOD and GSH/GSSG levels compared to the diabetic control group (P≤0.05). According to the difference in averages, this increase was more in the diabetic-supplement group. The level of GSSG in the diabetic-supplement and diabetic-supplement-exercise group was significantly decreased compared to the control-diabetic group (P≤0.05). According to the difference of the averages, this decrease was more in the diabetic-supplement-exercise group.
Conclusion: It seems that the combined effect of exercise training and sodium citrate supplementation is effective in improving diabetes and this effect is probably exerted by increasing GSH, GSH/GSSG and SOD and decreasing GSSG levels.
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