The effect of two months of intense intermittent exercise with sodium citrate supplementation on intracellular redox status and oxidative stress indices in diabetic rats

Document Type : Research Paper I Open Access I Released under (CC BY-NC 4.0) license

Authors

1 Department of Sport Physiology, Faculty of Educational Sciences and Psychology, University of Mohaghegh Ardabili, Ardabil, Iran

2 Department of Exercise Physiology, Faculty of Educational Sciences and Psychology, University of Mohaghegh Ardabili, Ardabil, Iran

3 Department of Sport Physiology, Faculty of Education and Psychology, University of Mohaghegh Ardabili, Ardabil, Iran

Abstract

Aim:      Alteration of cellular redox signal is effective in the incidence of diabetes and the development of its complications, so the aim of this study was to investigate the effect of two months high-intensity interval training with sodium citrate supplementation on intracellular redox status and oxidative stress indices in the heart of diabetic rats. Methods: This interventional study was performed on 50 white male rats (3 months old) with weight rang of 225-300 g in five groups: healthy control, diabetic control, diabetic-training, diabetic-sodium citrate supplementation, and diabetic-sodium citrate supplementation-training. Rats in both groups of training and training + supplementation performed interval training with an intensity of 90% of maximum running speed on a treadmill for 8 weeks, 5 days a week. In addition to the exercise, the rats in the exercise+supplement group received 15 mmol/L of sodium citrate supplement in the form of water soluble daily. Oxidative stress indices were measured using the ELISA kit with a coefficient of variation of 15%. Data were analyzed using one-way ANOVA and LSD post hoc test in SPPS26 statistical software (p<0.05). Results:  There was a significant difference between the healthy and diabetic groups in the levels of GSH, GSSG، GSH/GSSG, SOD (p<0.05). The level of GSH, GSH/GSSG, and SOD were lower in high-intensity interval training compared to diabetic control group (p<0.05) but sodium citrate supplementation significantly increased the level of these variables in diabetic rats. Significant reduction in the level of GSSG was observed in the diabetic-supplement and diabetic-supplement-exercise groups, compared to the diabetic-exercise and control-diabetic groups (p<0.05). The difference in the means showed that this decrease in GSSG level was more in the diabetic-supplement-exercise group compared to the other diabetic groups.
Conclusions The results of the present study showed that interval training with an intensity of 90% of the maximum running speed can increase oxidative stress indices in the heart of diabetic rats, so it is recommended to do this type of exercise with caution and using sodium citrate supplementation or other antioxidant supplements in diabetic patients.

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References

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Journal of Applied Health Studies in Sport Physiology
Volume 12, Issue 1
March 2025
Pages 121-135

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History

  • Receive Date: 22 April 2024
  • Revise Date: 02 August 2024
  • Accept Date: 04 August 2024

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