The effect of 8 weeks of resistance training with Spirulina platensis supplement on gene expression of oxidative parameters in the heart muscle of male rats

Document Type : Research Paper I Open Access I Released under (CC BY-NC 4.0) license

Authors

1 Department of Sports Sciences, Faculty of Literature and Humanities, Persian Gulf University, Bushehr, Iran.

2 Associate professor, Department of Sport Science, School of Literature and Humanities, Persian Gulf University, Boushehr, Iran

3 Department of Sport Sciences, School of Literature and Humanities, Persian Gulf University, Boushehr, Iran.

Abstract

Aim: Resistance training and spirulina supplementation change the expression of genes involved in the antioxidant pathway. The purpose of this study was to investigate the effects of resistance training and spirulina supplementation on the gene expression of some oxidative stress parameters in the heart muscle of male rats. Methods: 32 male rats weighing 150±20 grams were divided into four groups, which included control (CO), resistance training (RT), spirulina (SP), and resistance training with spirulina (RTS). Spirulina was orally administered to rats in SP and RTS groups at a dose of 200 mg/kg per day. The resistance training protocol consisted of eight weeks of climbing a 1-meter high ladder. The expression level of the dependent variables (SOD, CAT, GPx, and MDA) of the study was measured using the Real-time PCR method. We used of Two-way ANOVA in SPSS (P<0.05). Results:  The level of SOD gene expression decreased significantly in the supplement group (P=0.031), but in the training groups (P=0.974) and the interaction between supplementation and exercise was not significant (P=0.093). The decrease in CAT gene expression was not significant in any of the supplement (P=0.076), exercise (P=0.581) and the interaction of supplement and exercise (P=0.127) groups. The changes in GPx gene expression in the supplement group (P=0.032) decreased significantly, but in the exercise (P=0.326) and exercise and supplement (P=0.104) groups, the decrease in gene expression was not significant.  MDA gene expression changes were significantly reduced in the supplement group (P=0.009), while there was no significant decrease in gene expression in the exercise (P=0.416) and supplement plus exercise groups (P=0.051). Conclusions: The results of the study indicate that the consumption of spirulina platensis supplement alone has an effect in reducing oxidative stress in the heart muscle of male rats. 

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Main Subjects

References

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Journal of Applied Health Studies in Sport Physiology
Volume 11, Issue 2
August 2024
Pages 141-153

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History

  • Receive Date: 14 March 2024
  • Revise Date: 03 June 2024
  • Accept Date: 03 June 2024

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