Effect of omega-3 and vitamin E supplementation on some indices of collagen breakdown, damage and muscle contusion after explosive activity in young non-active

Document Type : Research Paper I Open Access I Released under (CC BY-NC 4.0) license

Authors

1 MSc in Exercise Physiology, Department of exercise physiology, Faculty of sport science, University of Guilan, Guilan, Iran.

2 Department of exercise physiology, Faculty of sport science, University of Guilan, Guilan, Iran.

Abstract

Aim:      Studies have shown that the consumption of omega-3 and vitamin E can have anti-inflammatory and antioxidant effects, and the use of these supplements is suggested in situations where muscle damage and inflammation occurs following exercise activities. The aim of this study was to determine the effect of short-term consumption of omega-3 and vitamin E supplements on some indices of collagen breakdown (hydroxylysine and serum hydroxyproline) and muscle damage (lactate dehydrogenase, creatine kinase) in untrained young people. Materials and methods:   Eight healthy and non-active men (age: 21.33±2.12 years, fat percentage: 19.02±4.46) were selected using statistical methods to determine the sample size. Subjects were randomly divided into two conditions of supplement and placebo. The research design was carried out in a blind way and the subjects did not know about the content of the supplement packages until the end of the research protocol. At the time of taking supplements, they took 3000 mg of omega-3 supplement in three meals daily (1000 mg each) and 400 IU of vitamin E supplement in one meal. Soft gels containing oral paraffin were consumed as a placebo. On the eighth day, for both conditions of the research, the subjects had a bout of intense anaerobic activity, including 8 sets of 12 repetitions of pair jumping on the box and 8 sets of 12 repetitions of landing from the 43 cm box with a one-minute rest between each set. Before supplementing and 24 and 48 hours after performing anaerobic activity, blood samples tests were taken from the subjects. Result:  The results showed that the values of lactate dehydrogenase (p=0.033) and hydroxyproline (p=0.046) increased significantly in 48 hours after anaerobic exercise compared to baseline. No significant change was observed in the values of creatine kinase (p=0.083) and hydroxylysine (p=0.088) variables. Conclusion: According to the findings, it seems that the combined consumption of omega-3 and vitamin E could not have a protective role on some indicators related to cell membrane and collagen damage, although more studies are needed for a more accurate conclusion.

Keywords

Main Subjects

References

  1. Atashak S, Sharafi H, Azarbayjani MA, Stannard SR, Goli M, Haghighi MM. Effect of omega-3 supplementation on the blood levels of oxidative stress, muscle damage and inflammation markers after acute resistance exercise in young athletes. Kinesiology. 2013;45:22-9. [In Persian]
  2. Souglis A, Bogdanis GC, Chryssanthopoulos C, Apostolidis N, Geladas ND. Time Course of Oxidative Stress, Inflammation, and Muscle Damage Markers for 5 Days After a Soccer Match: Effects of Sex and Playing Position. The Journal of Strength & Conditioning Research. 2018;32(7):2045-54.
  3. Ajam Zibad, M., TaheriChadorneshin, H., Abtahi Eivary, S. H. The effect of acute resistance exercise on serum levels of some inflammatory and muscle damage markers in inactive women. Journal of Practical Studies of Biosciences in Sport, 2016; 4(7): 76-88. [In Persian]
  4. Poprzecki S, Zajac A, Chalimoniuk M, Waskiewicz Z, Langfort J, Modification of blood antioxidant status and lipid profile in response to high-intensity endurance exercise after low doses of ω-3 polyunsaturated fatty acids supplementation in healthy volunteers. International journal of food sciences and nutrition, 2009; 60( 2): 67-79.
  5. Owens DJ, Twist C, Cobley JN, Howatson G, Close GL. Exercise-induced muscle damage: What is it, what causes it and what are the nutritional solutions? European Journal of Sport Science. 2019;19(1):71-85.
  6. Tofas T, Jamurtas AZ, Fatouros I, Nikolaidis MG, Koutedakis Y, Sinouris EA, et al. Plyometric Exercise Increases Serum Indices of Muscle Damage and Collagen Breakdown. The Journal of Strength & Conditioning Research.2008; 22(2): 490-6.
  7. de Oliveira DCX, Rosa FT, Simões-Ambrósio L, Jordao AA, Deminice R. Antioxidant vitamin supplementation prevents oxidative stress but does not enhance performance in young football athletes. Nutrition. 2019; 63-64: 29-35.
  8. Naghizadeh, H, Akbarzadeh, H. The Effect of Moderate Aerobic Exercise with Vitamin E Consumption on Total Anti-oxidant Capacity,Lipid Peroxidation and Muscle Damage in Active Male Students, Journal of Applied Exercise Physiology, 2013; 9(17): 27-42. [In Persian]
  9. Dutra MT, Alex S, Mota MR, Sales NB, Brown LE, Bottaro M. Effect of strength training combined with antioxidant supplementation on muscular performance. Applied Physiology, Nutrition, and Metabolism. 2018; 43(8): 775-81.
  10. Bloomer RJ. The Role of Nutritional Supplements in the Prevention and Treatment of Resistance Exercise-Induced Skeletal Muscle Injury. Sports Medicine. 2007; 37(6): 519-32.
  11. Petersen EW, Ostrowski K, Ibfelt T, Richelle M, Offord E, Halkjær-Kristensen J, et al. Effect of vitamin supplementation on cytokine response and on muscle damage after strenuous exercise. American Journal of Physiology-Cell Physiology. 2001; 280(6):C1570-5.
  12. Gammone MA, Riccioni G, Parrinello G, D’Orazio N. Omega-3 Polyunsaturated Fatty Acids: Benefits and Endpoints in Sport. Nutrients. 2019; 11(1): 46.
  13. Daryanoosh, F., daryanoosh, F., Mehrabani, D. Evaluating Inflammatory Index Changes and Muscle Injuries in Male Mice after 8 Weeks of Aerobic Exercise and Omega-3 Consumption. Journal of Sport Biosciences, 2012; 4(10): 77-94. [In Persian]
  14. Ramezani A, Djalali M, Yosefinejad A. Changes in Lipid Profiles among Patients with with Coronary Vascular Diseases Treated with Omega3 and Vitamin E: A Randomized Control Clinical Trial. J Mazandaran Univ Med Sci 2018; 28 (162) :1-11. [In Persian]
  15. Kim M-J, Park M, Jeong MK, Yeo J, Cho W-I, Chang P-S, et al. Radical scavenging activity and anti-obesity effects in 3T3-L1 preadipocyte differentiation of Ssuk (Artemisia princeps Pamp.) extract. Food Science and Biotechnology. 2010; 1992): 535-40.
  16. Urso ML, Clarkson PM. Oxidative stress, exercise, and antioxidant supplementation. Toxicology. 2003; 189: 41-54.
  17. Bruckner G, Webb P, Greenwell L, Chow C, Richardson D. Fish oil increases peripheral capillary blood cell velocity in humans. Atherosclerosis. 1987; 66(3):237-45.
  18. Hamedinia MR, Nikhbakht H, Rasaee MJ, Gaeini AA, Salami F. Effect of exhaution exercise on oxidative stress indicators and the enzyme ceratin kinase in student athletes. Olympic. 2002; 10(3-4): 39-47. [In Persian]
  19. Bloomer RJ, Goldfarb AH, McKenzie MJ, Oxidative stress response to aerobic exercise: comparison of antioxidant Medicine & science in sports & exercise, 2006; 38(6): 1098-1105.
  20. Algül S, Ugras S, Kara M. Comparative Evaluation of MDA Levels During Aerobic Exercise in Young Trained and Sedentary Male Subjects. Eastern J Med. 2018; 23: 98-101.
  21. Viña J, Gomez-Cabrera MC, Lloret A, Marquez R, Miñana JB, Pallardó FV, et al. Free radicals in exhaustive physical exercise: mechanism of production, and protection by antioxidants. IUBMB Life. 2000;50(4-5):271-7.
  22. Taghiyar, M., Ghiasvand, R., Feizi, A., Askari, G., Shokri, N. Effects of Vitamins C and E Supplementation on Muscle Damage and Oxidative Stress in Female Athletes: A Double-Blind Clinical Trial. Journal of Isfahan Medical School, 2013; 30(216): 2113-2124. [In Persian]
  23. Keong CC, Singh HJ, Singh R. Effects of palm vitamin e supplementation on exercise-induced oxidative stress and endurance performance in the heat. J Sports Sci Med. 2006; 5(4):629-39.
  24. -Filaire E, Massart A, Portier H, Rouveix, M, Rosado F, Bage AS, Gobert M, Durand D, Effect of 6 Weeks of n-3 fatty-acid supplementation on oxidative stress in Judo athletes. International journal of sport nutrition and exercise metabolism, 2010; 20.6: 496-506.
Journal of Applied Health Studies in Sport Physiology
Volume 11, Issue 2
August 2024
Pages 44-53

Files

History

  • Receive Date: 09 December 2023
  • Revise Date: 01 February 2024
  • Accept Date: 09 February 2024

Share

How to cite

Statistics