The Effect of L-Theanine Supplementation on Balance, Cognitive function, Anaerobic Power and Fatigue index in Trained Male Wushu Athletes

Document Type : Research Paper I Open Access I Released under (CC BY-NC 4.0) license

Authors

1 Department of Sports Sciences, Faculty of Humanities, University of Zanjan, Zanjan, Iran

2 Associate Professor of Exercise Physiology, Department of Sport Sciences, Faculty of Humanity, University of Znjan, Zanjan, Iran.

Abstract

Aim:      Supplements play an important role in improving the various performances of athletes. One of these supplements is L-theanine, which has many physiological effects. The aim of this study was to investigate the effect of L-theanine supplementation on balance, cognitive performance, anaerobic capacity and fatigue index of trained male wushu athletes. Methods: This research was conducted in a double-blind, cross-sectional and semi-experimental manner. The subjects included 11 people from Zanjan city, who participated in both the control (placebo) and experimental (supplement) groups with a certain time interval due to the spread of the corona disease and the reluctance of human subjects to participate in the research work.  The intended tests were performed twice, 3 days apart. Subjects in the placebo group received a 200 mg flour capsule and in the supplement group a 200 mg L-theanine capsule, and after 30 minutes (the time required for L-theanine to take effect), they performed balance, reaction time and RAST tests to measure anaerobic power (minimum, maximum, average) and fatigue index. For statistical analysis, Shapiro Wilk, covariance and paired t tests were used at the significance level of p=0.05. Results The results showed that 30 minutes after taking L-theanine supplement in humansubjects, the values ​​of balance (P=0.001), cognitive performance (P=0.010), minimum power (P=0.009)  improved and and there was no significant change in the subjects' fatigue (P=0.190), also the average power (P=0.017) was significantly reduced. Conclusions:  It seems that L-theanine supplement has significantly improved the balance, cognitive performance and minimum anaerobic power of trained male wushu athletes, but it did not have a significant effect on their fatigue and peak power, but the lack of improvement in the average power in the rest test probably indicates that this supplement in Reduction of cumulative fatigue from rapid repetitions is ineffective.
 

Keywords

Main Subjects


  1. Nakhaee M, Pakravan M. Prevalence and reasons for nutritional supplement use among athletes in body building gyms, Kerman 2012. Journal of Rafsanjan University of Medical Sciences. 2014;12(11):873-80 .[In Persian]
  2. Takeshima M, Miyazaki I, Murakami S, Kita T, Asanuma M. L-Theanine protects against excess dopamine-induced neurotoxicity in the presence of astrocytes. Journal of clinical biochemistry and nutrition. 2016:16-5.
  3. Li C, Tong H, Yan Q, Tang S, Han X, Xiao W, et al. L-Theanine improves immunity by altering TH2/TH1 cytokine balance, brain neurotransmitters, and expression of phospholipase C in rat hearts. Medical science monitor: international medical journal of experimental and clinical research. 2016;22:662.
  4. Guo X, Song C, Ho C-T, Wan X. Contribution of L-theanine to the formation of 2, 5-dimethylpyrazine, a key roasted peanutty flavor in Oolong tea during manufacturing processes. Food Chemistry. 2018;263:18-28.
  5. Mu W, Zhang T, Jiang B. An overview of biological production of L-theanine. Biotechnology advances. 2015;33(3-4):335-42.
  6. Hidese S, Ogawa S, Ota M, Ishida I, Yasukawa Z, Ozeki M, et al. Effects of L-theanine administration on stress-related symptoms and cognitive functions in healthy adults: a randomized controlled trial. Nutrients. 2019;11(10):362-9.
  7. Chatterjee S, Chatterjee A, Bandyopadhyay SK. L-Theanine: A prospective natural medicine. Int J Pharm Sci Rev Res. 2016;41:95-103.
  8. Yao J, Li J, Xiong D, Qiu Y, Shi G, Jin J-M, et al. Development of a highly efficient and specific l-theanine synthase. Applied microbiology and biotechnology. 2020;104(8):3417-31.
  9. Fu X, Cheng S, Liao Y, Xu X, Wang X, Hao X, et al. Characterization of L-theanine hydrolase in vitro and subcellular distribution of its specific product ethylamine in tea (Camellia sinensis). Journal of Agricultural and Food Chemistry. 2020;68(39):10842-51.
  10. Williams J, D’Cunha NM, Anstice N, McKune A, Naumovski N. Effect of Green Tea Amino Acid L-Theanine on Physiological Responses: A Protocol for Clinical Trial. Exploratory Research and Hypothesis in Medicine. 2020(000):1-8.
  11. Higashiyama A, Htay HH, Ozeki M, Juneja LR, Kapoor MP. Effects of l-theanine on attention and reaction time response. Journal of Functional Foods. 2011;3(3):171-8.
  12. Sakamoto FL, Ribeiro RMP, Bueno AA, Santos HO. Psychotropic effects of L-theanine and its clinical properties: From the management of anxiety and stress to a potential use in schizophrenia. Pharmacological research. 2019;147:104395.
  13. Türközü D, Şanlier N. L-theanine, unique amino acid of tea, and its metabolism, health effects, and safety. Critical reviews in food science and nutrition. 2017;57(8):1681-7.
  14. Adhikary R, Mandal V. L-theanine: A potential multifaceted natural bioactive amide as health supplement. Asian Pacific Journal of Tropical Biomedicine. 2017;7(9):842-8.
  15. JunFeng T, Zhi L, QunHua P, Li G, HaiPeng L. Evaluation on the alleviating physical fatigue function of theanine compound preparation. Journal of Tea Science. 2012;32(6):530-4.
  16. Kimura K, Ozeki M, Juneja LR, Ohira H. L-Theanine reduces psychological and physiological stress responses. Biological psychology. 2007;74(1):39-45.
  17. Ghafouri A, Mohammadi F, Ganji B. Relationship Between Selected Performance Tests and Non-contact Sports Injuries in Male Wushu Players. Physical Treatments-Specific Physical Therapy Journal. 2020;10(4):239-49. [In Persian]
  18. Moradiyan H, Parnow A, Eslami R. The acute effects of post-activation potentiation (PAP) warm-up protocols on anaerobic performance and lactate changes in female talo athletes. Journal of Sport and Exercise Physiology. 2018;11(2):87-96. [In Persian]
  19. Yavari A, Tanbakoosaz A, Rostami M, Khanian MYA, Esteki A, editors. Stability and control of human body motion during performing balance motion in Wushu. 2012 19th Iranian Conference of Biomedical Engineering (ICBME); 2012: IEEE.
  20. Artioli GG, Gualano B, Franchini E, Batista RN, Polacow VO, Lancha Jr AH. Physiological, performance, and nutritional profile of the Brazilian Olympic Wushu (kung-fu) team. The Journal of Strength & Conditioning Research. 2009;23(1):20-5.
  21. Enoka RM, Duchateau J. Translating fatigue to human performance. Medicine and science in sports and exercise. 2016;48(11):2228.
  22. Nikookheslat S, Vakili J, Mohammadi GA. Effect Of Acute Sodium Bicarbonate Supplementation On Anaerobic Performance In Elite And Amateur Male Cyclists. 2020. [In Persian]
  23. Rogers PJ, Smith JE, Heatherley SV, Pleydell-Pearce C. Time for tea: mood, blood pressure and cognitive performance effects of caffeine and theanine administered alone and together. Psychopharmacology. 2008;195(4):569-77.
  24. Rogers PJ, Smith JE, Heatherley SV, Pleydell-Pearce C. Time for tea: mood, blood pressure and cognitive performance effects of caffeine and theanine administered alone and together. Psychopharmacology. 2008;195:569-77.
  25. Ranjbar R, Kordi M, Gaeini AA. The effect of caffeine ingestion on anaerobic power; Fatigue index and blood lactate levels in boys athlete students. Journal of Sport Biosciences. 2009;1(1):123-36. [In Persian]
  26. Akins JS, Longo PF, Bertoni M, Clark NC, Sell TC, Galanti G, et al. Postural stability and isokinetic strength do not predict knee valgus angle during single-leg drop-landing or single-leg squat in elite male rugby union players. Isokinetics and Exercise Science. 2013;21(1):37-46.
  27. Yoto A, Motoki M, Murao S, Yokogoshi H. Effects of L-theanine or caffeine intake on changes in blood pressure under physical and psychological stresses. Journal of physiological anthropology. 2012;31(1):1-9.
  28. Haskell CF, Kennedy DO, Milne AL, Wesnes KA, Scholey AB. The effects of L-theanine, caffeine and their combination on cognition and mood. Biological psychology. 2008;77(2):113-22.
  29. Vuong QV, Bowyer MC, Roach PD. L‐Theanine: properties, synthesis and isolation from tea. Journal of the Science of Food and Agriculture. 2011;91(11):1931-9.
  30. Williams JL, Everett JM, D’Cunha NM, Sergi D, Georgousopoulou EN, Keegan RJ, et al. The effects of green tea amino acid L-theanine consumption on the ability to manage stress and anxiety levels: A systematic review. Plant Foods for Human Nutrition. 2020;75(1):12-23.
  31. Kim S, Jo K, Hong K-B, Han SH, Suh HJ. GABA and l-theanine mixture decreases sleep latency and improves NREM sleep. Pharmaceutical biology. 2019;57(1):64-72.
  32. Ellerbroek AC, Antonio J. Effects of pre-workout supplements on strength, endurance, and mood. Internet Journal of Allied Health Sciences and Practice. 2018;17(1):7.
  33. Behpour N, Hemati Ma. Acute Effect of Creatine Supplementation and Partial Sleep Deprivation on Performance and Cortisol Level of Male Athletes. 2014. [In Persian]
  34. Muir SW, Montero‐Odasso M. Effect of vitamin D supplementation on muscle strength, gait and balance in older adults: a systematic review and meta‐analysis. Journal of the American Geriatrics Society. 2011;59(12):229.
  35. Nobre AC, Rao A, Owen GN. L-theanine, a natural constituent in tea, and its effect on mental state. Asia Pacific journal of clinical nutrition. 2008;17.
  36. Ahmadi J, Hassani A, Donyai A. The effect of ginseng supplementation and six weeks of resistance training on aerobic and anaerobic power in sedentary male students. Journal of Practical Studies of Biosciences in Sport. 2015;3(5):45-55. [In Persian]
  37. Rahimi MR, Jafari A, Golpasandi H. The effect of caffeine ingestion on anaerobic performance and fatigue index in the morning and the evening times. Journal of Applied Health Studies in Sport Physiology. 2017;4(1):60-7. [In Persian]