Investigating the effect of eight weeks of high-intensity interval training and moderate-intensity continuous training with quercetin supplementation on the PLIN2 and ATGL gene expression in the liver of diabetic obese rats

Document Type : Research Paper I Open Access I Released under (CC BY-NC 4.0) license

Authors

1 Department of Exercise Physiology, Faculty of Educational Sciences and Psychology, University of Mohaghegh Ardabili, Ardabil, Iran.

2 Department of Exercise Physiology, Faculty of Educational Sciences and Psychology, University of Mohaghegh Ardabili, Ardabil, Iran

3 Department of Sport Management, Faculty of Educational Sciences and Psychology, University of Mohaghegh Ardabili, Ardabil, Iran.

Abstract

Aim: Obesity causes chronic inflammation, and inflammation causes insulin resistance in type 2 diabetes mellitus (T2DM). Thus, the aim of the present study was to investigate the effect of eight weeks of high-intensity interval training and moderate-intensity continuous training with quercetin supplementation on the gene expression of PLIN2 and ATGL in the liver of diabetic obese rats.
Methods: Forty two male Wistar rats at 8 weeks of age were purchased, and 36 of these animals were induced with T2DM after 8 weeks of high-fat diet. The rats were divided into the following groups (n=6): healthy control, diabetic control, diabetic quercetin supplementation, high-intensity interval training with diabetes (HIIT), moderate-intensity continuous training with diabetes (MICT), HIIT training with quercetin, and MICT training with quercetin. For one training group, 8 weeks of HIIT training and for the other 8 weeks of MICT training on the treadmill were performed. Seventy two hours after the last training session, liver tissues was isolated to check gene expression of PLIN2 and ATGL. Two-way analysis of variance with Tukey's post-hoc test (with a significance level of P<0.05) was used to analyze the data.
Results: After 8 weeks of intervention, a significant difference was seen only in the level of ATGL gene expression between four training groups (P=0/001), so that in HIIT and MICT training groups with quercetin its levels was statistically more than two other training groups with the significant levels of (P=0/001) and (P=0/015), respectively. Conclusions: Apparently, the implementation of both types of exercise has an effect on ATGL and PLIN2 gene expression, and when exercise is combined with quercetin, this supplementation can indirectly affect ATGL gene expression in T2DM conditions by controlling blood levels.

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Main Subjects

References

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Journal of Applied Health Studies in Sport Physiology
Volume 11, Issue 1
March 2024
Pages 237-250

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History

  • Receive Date: 10 November 2023
  • Revise Date: 19 January 2024
  • Accept Date: 20 January 2024

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