The effect of high-intensity interval training (HIIT) on GLUT-3 expression and lactate levels in testicular tissue of obese rats

Articles in Press

Document Type : Research Paper I Open Access I Released under (CC BY-NC 4.0) license

Authors

1 Department of Exercise Physiology and Corrective Exercises, Faculty of Sport Sciences, Urmia University, Urmia, Iran

2 Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran

Abstract

Aim: In the obesity condition, the supply of testicular tissue energy substrate is under negative adjustment, and exercise training is an efficient strategy to deal with the adverse effects of obesity. The purpose of this study was to investigate the effect of 12 weeks of high-intensity interval training (HIIT) on the glucose transporter 3 (GLUT-3) gene expression and lactate levels in testicular tissue of obese male Wistar rats. Methods: The present study was experimental. 18 rats were randomly divided into 3 groups: 1) control, 2) HFD-sole, and 3) HFD+HIIT (n=6/group). The HFD and HFD+HIIT groups were fed with high-fat diet (HFD) from the beginning to the end of the study (24 weeks), and the control group was fed standard food from the beginning to the end of the study. From the 12th week of the study, the HFD+HIIT group was trained for 12 weeks, and five sessions per week. HIIT was performed with a 20-degree slope, 13 intervals, and an intensity of 85-90% of the maximum running speed (Smax). GLUT-3 mRNA levels were measured using qRT-PCR technique and lactate levels were measured by ELISA method. One-way ANOVA followed by Tukey post-hoc tests was performed for analyses of quantitative findings regarding molecular findings were conducted. Results: HFD-induced obesity exhibited a remarkable (p<0.05) reduction in testis GLUT-3 mRNA and lactate levels, in contrast, HIIT group represented a remarkable increment (p<0.05) GLUT-3 expression and lactate levels. Conclusions: Our result confirms that HIIT training can potentially ameliorate the detrimental effects of HFD-induced obesity on the testis. In this regard, it seems that HIIT with up-regulation of glucose transporter (GLUT-3) may help to improve the process of glucose to lactate converting and facilitate access to the desired testicular tissue substrate.

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Journal of Applied Health Studies in Sport Physiology

Articles in Press, Accepted Manuscript
Available Online from 02 December 2023

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History

  • Receive Date: 18 October 2023
  • Revise Date: 01 December 2023
  • Accept Date: 02 December 2023

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