The effect of eight weeks of high-intensity interval swimming training on the expression of PGC-1α and IL-6 proteins and memory function in brain hippocampus in rats with non-alcoholic steatohepatitis induced by high fat diet

Articles in Press

Document Type : Research Paper I Open Access I Released under (CC BY-NC 4.0) license

Authors

1 Master Student of Exercise Physiology, Faculty of Sport Sciences and Health, University of Tehran, Tehran, Iran.

2 Professor of Exercise Physiology, Faculty of Sport Sciences and Health, University of Tehran, Tehran, Iran.

3 Associate Professor in Exercise Physiology, Faculty of Educational Sciences and Psychology, Department of Sports Sciences, Shiraz University, Shiraz, Iran

Abstract

Aim: Nonalcoholic steatohepatitis (NASH) is the result of excessive accumulation of triglycerides in liver cells, which is associated with a decrease in PGC-1α and an increase in inflammatory cytokines. The present study was conducted with the aim of investigating the effect of eight weeks of high-intensity interval swimming training on the expression of PGC-1α and IL-6 proteins and memory function in the brain hippocampus in rats with non-alcoholic steatohepatitis induced by a high-fat diet. Methods: The present research was experimental. 40 male Sprague-Dawley rats were divided into two groups: healthy (n=20) and high-fat diet (HFD) (n=20). The HFD group received a high-fat diet for eight weeks with the aim of inducing the disease. After proving the disease, the rats were divided into four groups: control-healthy (n=9), training-healthy (n=9), control-disease (n=9), and training-disease (n=9). The training groups performed eight weeks of high-intensity interval swimming exercises (three sessions per week, each session lasting 30 minutes). In the end, PGC-1α and IL-6 proteins in the hippocampus of the brain were measured by the ELISA method, and memory performance was evaluated by the Morris water Maze behavioral test. One-way analysis of variance with the Bonferroni post hoc test was used for data analysis (p<0.05) and all statistical methods were performed using spss version 26 software. Results: The highest and lowest amount of PGC-1α protein was observed in training-healthy and control-disease groups, respectively. Also, in the IL-6 variable, the highest and lowest levels were observed in the control-disease and training-healthy groups, respectively. There was also a significant difference between the groups in the variable of memory performance (P<0.05). Conclusion: high-intensity interval swimming training in NASH can lead Increase in PGC-1α and decrease IL-6 in the brain hippocampus and improve memory function.

Keywords

References

  1. Rives C, Fougerat A, Ellero-Simatos S, Loiseau N, Guillou H, Gamet-Payrastre L, et al. Oxidative stress in NAFLD: Role of nutrients and food contaminants. Biomolecules. 2020;10(12):1702.
  2. Mirzarazi Dehaghi R, Safavi Homami S, Alaei H. The Comparison of Immobilization Stress on Learning and Spatial Memory in Young and Old Male Rats. Motor Behavior. 2018;10(32):165-78. [In Persian]
  3. Ross A, Bruggeman E, Kasumu A, Mielke J, Parent M. Non-alcoholic fatty liver disease impairs hippocampal-dependent memory in male rats. Physiology & behavior. 2012;106(2):133-41.
  4. Kjærgaard K, Mikkelsen ACD, Wernberg CW, Grønkjær LL, Eriksen PL, Damholdt MF, et al. Cognitive dysfunction in non-alcoholic fatty liver disease—current knowledge, mechanisms and perspectives. Journal of Clinical Medicine. 2021;10(4):673.
  5. Tobey T, Mondon C, Zavaroni I, Reaven G. Mechanism of insulin resistance in fructose-fed rats. Metabolism. 1982;31(6):608-12.
  6. Febbraio MA, Pedersen BK. Muscle‐derived interleukin‐6: mechanisms for activation and possible biological roles. The FASEB journal. 2002;16(11):1335-47.
  7. Chalasani N, Younossi Z, Lavine JE, Charlton M, Cusi K, Rinella M, et al. The diagnosis and management of nonalcoholic fatty liver disease: practice guidance from the American Association for the Study of Liver Diseases. Hepatology. 2018;67(1):328-57.
  8. Sullivan S, Kirk EP, Mittendorfer B, Patterson BW, Klein S. Randomized trial of exercise effect on intrahepatic triglyceride content and lipid kinetics in nonalcoholic fatty liver disease. Hepatology. 2012;55(6):1738-45.
  9. Brunner KT, Henneberg CJ, Wilechansky RM, Long MT. Nonalcoholic fatty liver disease and obesity treatment. Current obesity reports. 2019;8:220-8.
  10. Bacchi E, Negri C, Targher G, Faccioli N, Lanza M, Zoppini G, et al. Both resistance training and aerobic training reduce hepatic fat content in type 2 diabetic subjects with nonalcoholic fatty liver disease (the RAED2 Randomized Trial). Hepatology. 2013;58(4):1287-95.
  11. Lunt H, Draper N, Marshall HC, Logan FJ, Hamlin MJ, Shearman JP, et al. High intensity interval training in a real world setting: a randomized controlled feasibility study in overweight inactive adults, measuring change in maximal oxygen uptake. PloS one. 2014;9(1):e83256.
  12. Khalafi M, Mohebbi H, Karimi P. High-intensity interval training increases mitochondria biogenesis in adipose tissue and improves insulin resistance in high fat diet-induced obese rat. International Journal of Applied Exercise Physiology. 2019;8(1):43-50. [In Persian]
  13. Bressel E, Louder TJ, Raikes AC, Alphonsa S, Kyvelidou A. Water immersion affects episodic memory and postural control in healthy older adults. Journal of Geriatric Physical Therapy. 2019;42(4):E1-E6.
  14. Nagle EF, Sanders ME, Franklin BA. Aquatic high intensity interval training for cardiometabolic health: benefits and training design. American journal of lifestyle medicine. 2017;11(1):64-76.
  15. Zou Y, Li J, Lu C, Wang J, Ge J, Huang Y, et al. High-fat emulsion-induced rat model of nonalcoholic steatohepatitis. Life sciences. 2006;79(11):1100-7.
  16. Chen B, Ma Y, Xue X, Wei J, Hu G, Lin Y. Tetramethylpyrazine reduces inflammation in the livers of mice fed a high fat diet. Molecular Medicine Reports. 2019;19(4):2561-8.
  17. Azimi Dokht SMA, Gharakhanlou R, Naghdi N, Khodadadi D, Zare Zade Mehrizi AA. The effect of the treadmill running on genes expression ofthePGC-1α, FNDC5 and BDNF in hippocampus of male rats. Journal of Practical Studies of Biosciences in Sport. 2019;7(14):91-101. [In Persian]
  18. Ramos-Filho D, Chicaybam G, de-Souza-Ferreira E, Guerra Martinez C, Kurtenbach E, Casimiro-Lopes G, et al. High intensity interval training (HIIT) induces specific changes in respiration and electron leakage in the mitochondria of different rat skeletal muscles. PloS one. 2015;10(6):e0131766.
  19. Liu Y, Dong G, Zhao X, Huang Z, Li P, Zhang H. Post-exercise effects and long-term training adaptations of hormone sensitive lipase lipolysis induced by high-intensity interval training in adipose tissue of mice. Frontiers in Physiology. 2020;11:535722.
  20. Barzegar H, Vosadi E, Borjian Fard M. The Effect of Different Modes of Training on Plasma Levels of IL-6 and IL-10 in Male Mature Wistar Rats. Journal of Sport Biosciences. 2017;9(2):171-81. [In Persian]
  21. Kern L, Mittenbühler MJ, Vesting AJ, Ostermann AL, Wunderlich CM, Wunderlich FT. Obesity-induced TNFα and IL-6 signaling: the missing link between obesity and inflammation—driven liver and colorectal cancers. Cancers. 2018;11(1):24.
  22. Pietrelli A, Matković L, Vacotto M, Lopez-Costa J, Basso N, Brusco A. Aerobic exercise upregulates the BDNF-Serotonin systems and improves the cognitive function in rats. Neurobiology of learning and memory. 2018;155:528-42.
  23. Nassir F. NAFLD: Mechanisms, treatments, and biomarkers. Biomolecules. 2022;12(6):824.
  24. Sharafi Dehrhm F, Soori R, Abbasian S, Rastegar MM M. The effect of high-intensity exercise training on serum levels and gene expression of Tfam and PGC1α in hippocampus of male rats. Sport Physiology & Management Investigations. 2019;11(2):75-85. [In Persian]
  25. Bagherian M, Banaeifar A, Arshadi S, Azarbayjani M. THE EFFECT OF EXERCISE TYPE ON THE EXPRESSION OF PGC-1A AND HEART TISSUE TRIGLYCERIDE CONTENT IN RATS WITH FATTY LIVER (NAFLD). Studies in Medical Sciences. 2020;31(4):282-94. [In Persian]
  26. Nunes-Souza V, Alenina N, Qadri F, Penninger JM, Santos RAS, Bader M, et al. CD36/sirtuin 1 axis impairment contributes to hepatic steatosis in ACE2-deficient mice. Oxidative Medicine and Cellular Longevity. 2016;2016.
  27. Rius-Pérez S, Torres-Cuevas I, Millán I, Ortega ÁL, Pérez S. PGC-1α, inflammation, and oxidative stress: an integrative view in metabolism. Oxidative medicine and cellular longevity. 2020;2020.
  28. Rezaee Z, Marandi SM, Alaei H, Esfarjani F. The effect of endurance training on gene expression of PGC-1α, BDNF and learning disorders in the hippocampus of aging rats. Sport physiology. 2019;11(43):91-104. [In Persian]
  29. Hofmann T, Elbelt U, Stengel A. Irisin as a muscle-derived hormone stimulating thermogenesis–a critical update. Peptides. 2014;54:89-100.
  30. Zhang Q, Wu Y, Sha H, Zhang P, Jia J, Hu Y, et al. Early exercise affects mitochondrial transcription factors expression after cerebral ischemia in rats. International Journal of Molecular Sciences. 2012;13(2):1670-9.
  31. Alahmadi M. High-intensity interval training and obesity. J Nov Physiother. 2014;4(3):211.
  32. Ghorbanian B, Egtesadi S. The effect of high intensity interval training (HIIT) on serum levels of selenoprotein P and glycemic and body composition indices in women with nonalcoholic fatty liver disease. Journal of Applied Health Studies in Sport Physiology. 2022;9(1):12-21. [In Persian]
  33. Bagheri MH, Azamian Jazi A, Bani Talebi E, Nasr-Esfahani MH. The effects of eight weeks of high intensity interval training on expression of PPARγ and liver TG in rats with fatty liver disease. Sport Physiology. 2020;12(47):113-32. [In Persian]
  34. Forbes SC, Candow DG, Smith-Ryan AE, Hirsch KR, Roberts MD, VanDusseldorp TA, et al. Supplements and nutritional interventions to augment high-intensity interval training physiological and performance adaptations—a narrative review. Nutrients. 2020;12(2):390.

 

Journal of Applied Health Studies in Sport Physiology

Articles in Press, Accepted Manuscript
Available Online from 21 July 2023

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History

  • Receive Date: 30 April 2023
  • Revise Date: 12 July 2023
  • Accept Date: 12 July 2023

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