The Effect of Aerobic Exercise and Green Coffee on Adipose Tissue Thermogenesis in Pre-Diabetic Mice

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Document Type : Research Paper I Open Access I Released under (CC BY-NC 4.0) license

Authors

1 Department of Physical Education and Sport Science ,University of Sistan and Baluchistan, Zahedan, Iran

2 Department of Sports Sciences, Faculty of Educational Sciences and Psychology, University of Sistan and Baluchistan, Zahedan, Iran

3 Professor of Sports Sciences Department, Faculty of Sports Sciences, Isfahan University,Isfahan, Iran

4 Department of Physical Education and Sport Science , Isfahan University, Isfahan, Iran

5 Professor of Embryology, Royan Biotechnology Research Institute, Isfahan, Iran

Abstract

Aim: Prediabetes is a high-risk condition for type2diabetes and has a effect on fat and glucose metabolism. The aim of this study was to determine the effect of aerobic exercise and green coffee on the expression of thermogenic genes of uncoupling protein1(UCP1) and iodothyronine deiodinase2(DIO2) in the adipose tissue of prediabetic mice. Methods: Out of 25 male C57bl-6 mice aged 4 weeks, 5 were considered as control group and 20 were considered as prediabetes group. In the first stage, the control group was given a normal diet and the prediabetic group was given a high-fat diet for 12 weeks. After induction of prediabetes, in the second phase of the study, mice were randomly divided into groups: control(Ctrl), prediabetes(PD), prediabetes-aerobic exercise(EX), prediabetes-green coffee(GC) and prediabetes-green coffee-aerobic exercise(EX/GC). Mice of EX and EX/GC groups ran on a treadmill for 10 weeks. Also, mice in EX/GC and GC groups were subjected to green coffee gavage for 10 weeks. The expression of UCP1 and DIO2 genes in subcutaneous fat tissue was measured by real time PCR method and after quantification, gene expression values were analyzed using ΔΔCt-2 formula and then t-test, ANOVA and Tukey's post hoc test. Results: The results showed that12weeks of high-fat diet increased fasting blood sugar and exposed mice to prediabetes. Also, the relative expression of UCP1 and DIO2 in adipose tissue of EX group was significantly increased compared to other groups(P=0/000). Conclusion: The results showed that aerobic exercise and green coffee can have beneficial effects on the expression of UCP1 and DIO2 genes in the subcutaneous adipose tissue of prediabetic mice and may activate thermogenesis in this tissue and thereby reducing the risks of prediabetes.

Keywords

References

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Journal of Applied Health Studies in Sport Physiology

Articles in Press, Accepted Manuscript
Available Online from 11 May 2023

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History

  • Receive Date: 27 December 2022
  • Revise Date: 08 May 2023
  • Accept Date: 09 May 2023

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