The effect of three methods of HIT, HIIT and MIT training on leptin and lipoprotein lipase gene expression in subcutaneous fat tissue of male rats.

Document Type : Research Paper I Open Access I Released under (CC BY-NC 4.0) license

Authors

1 4. Master, Department of Exercise Physiology, Payame Noor University, Tehran PO Box 3697-19395, Iran

2 Assistant Professor, Department of physical education, Payame Noor University, PO BOX 19395-3697, Tehran, IRAN.

3 Assistant Professor, Department of physical education, Payame Noor University, PO BOX 19395-3697, Tehran, IRAN

4 Assistant Professor, Department of Physical Education, Department of Educational Sciences and Psychology, Payame Noor University, Tehran PO Box 3697-19395, Iran.

Abstract

    
Aim: Leptin and lipoprotein lipase are important factors in the process of fat metabolism and energy homeostasis, and the effect of different methods of exercise training has been less studied. The aim of this study was the effect of three types of HIT, HIIT and MIT exercises on leptin and lipoprotein lipase gene expression in the subcutaneous fat tissue of male rats. Methods: 32 male rats with an average weight of 237 ± 25 grams were prepared. Mice were randomly divided into four equal groups of control, moderate-intensity aerobic training, high-intensity aerobic training, and high-intensity intermittent aerobic training. The training groups participated in training programs for 8 weeks. The expression of leptin and lipoprotein lipase genes was determined using RT-PCR laboratory method. To investigate the difference between groups of variables, one-way analysis of variance and Tukey's post hoc test and SPSS-24 software were used.. Results:The results showed that there was a significant difference in the expression of leptin and lipoprotein lipase between the groups (control, HIT, HIIT and MIT)(p=0.001).Tukey's test results showed that although performingHIT, HIIT and MIT exercises decreased leptin gene expression (p=0.001) and increased lipoprotein lipase compared to the control group(p=0.001).However, no significant difference was observed between training groups(HIT, HIIT and MIT)in leptin and lipoprotein lipase changes.Conclusion:The results of the present study showed that HIT, HIIT and MIT all cause changAes in leptin and lipoprotein lipase gene expression, but it seems that the changes in leptin and lipoprotein lipase in HIT exercises were more than in MIT and HIIT.
 

Keywords

Main Subjects

Publisher: Azarbaijan Shahid Madani University          Copyright © The Authors   

   

 

This is an open access article distributed under the following Creative Commons license: Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)

References

  1. Khodamoradi K, Khosravizadeh Z, Seetharam D, Mallepalli S, Farber N, Arora H. The role of leptin and low testosterone in obesity. International journal of impotence research. 2022:1-10.
  2. Pourranjbar M, Kazemi A, Arab M-j. Effect of High Intensity Interval Training (HIIT) on visceral and subcutaneous levels of Leptin and plasma insulin and glucose in Wistar male Rats. Scientific Journal of Kurdistan University of Medical Sciences. 2019;23(6):89-100. [ In pesian ]
  3. Farahati S, Hosseini SRA, Moazzami M, Daloee MH, Daloee SH. The Impact of High-Intensity Interval Training Versus Moderate-Intensity Continuous Training on Carotid Intima-Media Thickness and Ankle-Brachial Index in Middle-Aged Women. International journal of preventive medicine. 2020;11:62-.
  4. Khademosharie M, Hosseini-Kakhk SA, Hamedinia MR, Amiri Parsa T. The effect of acute exercise on adipose tissue LPL gene expression and LPL activity in rats. Iranian South Medical Journal. 2014;17(2):120-9. [ In pesian ]
  5. Wu SA, Kersten S, Qi L. Lipoprotein Lipase and Its Regulators: An Unfolding Story. Trends in Endocrinology & Metabolism. 2021;32(1):48-61.
  6. Young SG, Fong LG, Beigneux AP, Allan CM, He C, Jiang H, et al. GPIHBP1 and lipoprotein lipase, partners in plasma triglyceride metabolism. Cell metabolism. 2019;30(1):51-65.
  7. Kersten S. Physiological regulation of lipoprotein lipase. Biochimica et Biophysica Acta (BBA)-Molecular and Cell Biology of Lipids. 2014;1841(7):919-33.
  8. Singh AK, Aryal B, Chaube B, Rotllan N, Varela L, Horvath TL, et al. Brown adipose tissue derived ANGPTL4 controls glucose and lipid metabolism and regulates thermogenesis. Molecular metabolism. 2018;11:59-69.
  9. Catoire M, Alex S, Paraskevopulos N, Mattijssen F, Evers-van Gogh I, Schaart G, et al. Fatty acid-inducible ANGPTL4 governs lipid metabolic response to exercise. Proceedings of the National Academy of Sciences. 2014;111(11):E1043-E52.
  10. Kiens B, Roepstorff C, Glatz JF, Bonen A, Schjerling P, Knudsen J, et al. Lipid-binding proteins and lipoprotein lipase activity in human skeletal muscle: influence of physical activity and gender. Journal of applied physiology (Bethesda, Md : 1985). 2004;97(4):1209-18.
  11. Barzegari A, Asad M, Dashti KhavIdaki MH, Eri E. The effect of high-Intensity Interval Training and Moderate-Intensity Continuous Training on serum Irisin levels and insulin resistance in overweight men. Journal of Practical Studies of Biosciences in Sport. 2022;13(26):1-11. [ In pesian ]
  12. Jahandideh AA, Rohani H, Rajabi H, Shariatzade Joneidi M. Effect of 8-weeks of Combined Exercise Training on Plasma Leptin and Adiponectin levels in Obese Boys. Journal of Applied Health Studies in Sport Physiology. 2021;8(2):25-33. [ In pesian ]
  13. MOHITI AJ, AFKHAMI AM, EDGHI H. Comparitive Study of Serum Leptin Levels in Diabetic Obese Patients and Non-Diabetic Obese Individuals. 2004[ In pesian ].
  14. Booth A, Magnuson A, Fouts J, Foster MT. Adipose tissue: an endocrine organ playing a role in metabolic regulation. Hormone molecular biology and clinical investigation. 2016;26(1):25-42.
  15. Buchan DS, Ollis S, Young JD, Thomas NE, Cooper SM, Tong TK, et al. The effects of time and intensity of exercise on novel and established markers of CVD in adolescent youth. American Journal of Human Biology. 2011;23(4):517-26.
  16. Khanevari T, Rohani H, Vakili J, Sari sarraf V. Effect of High-Intensity Interval Training on Leptin, Adiponectin, and Leptin/Adiponectin Ratio in Overweight Adolescent Boys. scientific magazine yafte. 2021;23(3):43-56. [ In pesian ]
  17. Ngayimbesha A, Bizimana J, Gakima M. Effect of eight weeks of exercise training on lipid profile and insulin sensitivity in obese person. Int J Sports Exerc Med. 2019;5:119-25.
  18. Caldeira RS, Panissa VLG, Inoue DS, Campos EZ, Monteiro PA, de Melo Giglio B, et al. Impact to short-term high intensity intermittent training on different storages of body fat, leptin and soluble leptin receptor levels in physically active non-obese men: A pilot investigation. Clinical nutrition ESPEN. 2018;28:186-92.
  19. Franz MJ, Powers MA, Leontos C, Holzmeister LA, Kulkarni K, Monk A, et al. The evidence for medical nutrition therapy for type 1 and type 2 diabetes in adults. Journal of the American Dietetic Association. 2010;110(12):1852-89.
  20. Keating SE, Johnson NA, Mielke GI, Coombes JS. A systematic review and meta‐analysis of interval training versus moderate‐intensity continuous training on body adiposity. Obesity reviews. 2017;18(8):943-64.
  21. Liu Y, Dong G, Zhao X, Huang Z, Li P, Zhang H. Post-exercise effects and long-term training adaptations of hormone sensitive lipase lipolysis induced by high-intensity interval training in adipose tissue of mice. Frontiers in Physiology. 2020;11:1503.
  22. Wewege M, Van Den Berg R, Ward R, Keech A. The effects of high‐intensity interval training vs. moderate‐intensity continuous training on body composition in overweight and obese adults: a systematic review and meta‐analysis. Obesity Reviews. 2017;18(6):635-46.
  23. Zouhal H, Saeidi A, Kolahdouzi S, Ahmadizad S, Hackney AC, Abderrahmane AB. Exercise and Training Effects on Appetite-Regulating Hormones in Individuals with Obesity. Endocrinology of Physical Activity and Sport: Springer; 2020. p. 535-62.
  24. Zheng D, Wooter MH, Zhou Q, Dohm GL. The effect of exercise on ob gene expression. Biochemical and biophysical research communications. 1996;225(3):747-50.
  25. Atanassova P, Delchev S, Georgieva K, Koeva Y, editors. Lipoprotein lipase enzyme histochemical activity in subcutaneous adipose tissue and skeletal muscle of the rat after submaximal exercise training. J Conference of Biol; 2005.
  26. Seip RL, Angelopoulos TJ, Semenkovich CF. Exercise induces human lipoprotein lipase gene expression in skeletal muscle but not adipose tissue. American Journal of Physiology-Endocrinology And Metabolism. 1995;268(2):E229-E36.
  27. asad m, Torabi Z, Barzegari A, Amouzad Mahdirejei H. Comparison of four exercise training protocol for eight weeks on expression of some antioxidant enzymes in heart tissue of rats. Journal of Sport Biosciences. 2021;12(4):473-92. [ In pesian ]
  28. Kim D-H, Kim S-H, Kim W-H, Moon C-R. The effects of treadmill exercise on expression of UCP-2 of brown adipose tissue and TNF-α of soleus muscle in obese Zucker rats. Journal of Exercise Nutrition & Biochemistry. 2013;17(4):199.
  29. Tashakkori Ghanbarian M, Naghibi S, Shariatzadeh Jonaidi M, Ansari S. The Effects of Aerobic Training with Different Intensity on FoxO1 and STRA13 Genes Expression in Subcutaneous Fat Tissue of Male Wistar Rat. Journal of Ardabil University of Medical Sciences. 2021;21(4):429-40. [ In pesian ]
  30. Khalafi M, Fatemeh Shabkhiz S, Zolfaghari M, Zarei Y. The effect of two types of exercise on serum chemerin in diabetic male rats. Qom University of Medical Sciences Journal. 2016;10(8):27-35. [ In pesian ]
  31. Haram PM, Kemi OJ, Lee SJ, Bendheim MØ, Al-Share QY, Waldum HL, et al. Aerobic interval training vs. continuous moderate exercise in the metabolic syndrome of rats artificially selected for low aerobic capacity. Cardiovascular research. 2009;81(4):723-32.
  32. García-Hermoso A, Ceballos-Ceballos R, Poblete-Aro C, Hackney A, Mota J, Ramírez-Vélez R. Exercise, adipokines and pediatric obesity: a meta-analysis of randomized controlled trials. International journal of obesity. 2017;41(4):475-82.
  33. sheikholeslami-vatani d, Ebrahimi A. The Effect of Moderate-Intensity Continuous Training Vs. High-Intensity Interval Training on Visceral and Subcutaneous Fats in Obese Women. Journal of Rafsanjan University of Medical Sciences. 2018;16(11):999-1012. [ In pesian ]
  34. Inoue DS, Panissa VL, Antunes BM, Oliveira FP, Malta RB, Caldeira RS, et al. Reduced leptin level is independent of fat mass changes and hunger scores from high-intensity intermittent plus strength training. The Journal of Sports Medicine and Physical Fitness. 2017;58(7-8):1045-51.
  35. Mabhout Moghadam T, Mosaferi Ziaaldini M, Fathi M, Attarzadeh Hoseini SR. Review the Effect of High Intensity Interval Training on Obesity- Related Hormones. Researches in Sport Sciences and Medical Plants. 2020;1(1):1-18. [ In pesian ]
  36. Jürimäe J. Ghrelin responses to acute exercise and training. Endocrinology of Physical Activity and Sport: Springer; 2020. p. 193-207.
  37. Bramlett S, Zhou J, Harris R, Hendry S, Witt T, Zachwieja J. Does beta(3)-adrenoreceptor blockade attenuate acute exercise-induced reductions in leptin mRNA? Journal of applied physiology (Bethesda, Md : 1985). 1999;87:1678-83.
  38. Hackney AC, Constantini NW. Endocrinology of physical activity and sport: Springer; 2020.
  39. Eliakim A, Nemet D. Exercise and the GH-IGF-I axis. Endocrinology of physical activity and sport: Springer; 2020. p. 71-84.
  40. Ondrak KS. Sex Differences in Energy Balance and Weight Control. Endocrinology of Physical Activity and Sport: Springer; 2020. p. 161-70.
  41. Cooper KM, Ackerman KE. Endocrine implications of relative energy deficiency in sport. Endocrinology of Physical Activity and Sport: Springer; 2020. p. 303-20.
  42. Vatansever-Ozen S, Tiryaki-Sonmez G, Bugdayci G, Ozen G. The effects of exercise on food intake and hunger: Relationship with acylated ghrelin and leptin. Journal of sports science & medicine. 2011;10(2):283.
  43. Martins C, Kulseng B, Rehfeld J, King N, Blundell J. Effect of chronic exercise on appetite control in overweight and obese individuals. Medicine and science in sports and exercise. 2013;45(5):805-12.
  44. Prencipe N, Bona C, Lanfranco F, Grottoli S, Benso AS. The Effects of Altitude on the Hormonal Response to Physical Exercise. Endocrinology of Physical Activity and Sport: Springer; 2020. p. 341-62.
  45. Seip RL, Semenkovich CF. Skeletal muscle lipoprotein lipase: molecular regulation and physiological effects in relation to exercise. Exercise and sport sciences reviews. 1998;26:191-218.
  46. Gasparotto J, Martinello KDB, Oliveira MLS, Somensi N, Schnorr CE. Aerobic Exercise Sensitizes Brown Adipose Tissue and Mobilizes Fat Oxidation in Obese Rats. J Nutr Obes. 2021;2:105.
  47. Lin X, Chavez MR, Bruch RC, Kilroy GE, Simmons LA, Lin L, et al. The effects of a high fat diet on leptin mRNA, serum leptin and the response to leptin are not altered in a rat strain susceptible to high fat diet-induced obesity. The Journal of nutrition. 1998;128(10):1606-13.
  48. Zouhal H, Lemoine-Morel S, Mathieu M-E, Casazza GA, Jabbour G. Catecholamines and obesity: effects of exercise and training. Sports Medicine. 2013;43(7):591-600.
  49. Ryan AS, Ortmeyer HK. Insulin suppression of fatty acid skeletal muscle enzyme activity in postmenopausal women, and improvements in metabolic flexibility and lipoprotein lipase with aerobic exercise and weight loss. International Journal of Obesity. 2019;43(2):276-84.
  50. Lebrun CM, Joyce SM, Constantini NW. Effects of female reproductive hormones on sports performance. Endocrinology of physical activity and sport: Springer; 2013. p. 281-322.
  51. Perrone B, Ruffo P, Zelasco S, Giordano C, Morelli C, Barone I, et al. LPL, FNDC5 and PPARγ gene polymorphisms related to body composition parameters and lipid metabolic profile in adolescents from Southern Italy. Journal of Translational Medicine. 2022;20(1):1-12.
  52. Al-Samawi RI, Smaism MF. Association of the lipoprotein lipase and Apolipoprotein C-II gene polymorphisms with risk of dyslipidemia in smokers and non-smokers male. Indian Heart Journal. 2022;74(1):45-50.
  53. Abbasi J. For fat burning, interval training beats continuous exercise. Jama. 2019;321(22):2151-2. [ In pesian ]
  54. Hamzehzadeh Borujeni E, Nazarali P, Naghibi S. Effect of Four Weeks HIT on the Levels of GH, IGFBP-3, IGF-1 and Serum Cortisol and some Performance Indicators in Iran Women National Basketball Team. Journal of Sport Biosciences. 2013;5(4):35-48. [ In pesian ]
Journal of Applied Health Studies in Sport Physiology
Volume 10, Issue 1
April 2023
Pages 97-111

Files

History

  • Receive Date: 03 October 2022
  • Revise Date: 21 January 2023
  • Accept Date: 22 January 2023

Share

How to cite

Statistics