Apoptotic changes of cardiac tissue after HIIT and thyme honey in type 2 diabetic rats

Document Type : Research Paper I Open Access I Released under (CC BY-NC 4.0) license

Authors

1 1. Ph.D. Student of Exercise physiology, department of physical education and sport science, Science and research branch, Islamic Azad University, Tehran, Iran.

2 Department of physical education and sport science, Science and research branch, Islamic Azad University, Tehran, Iran

Abstract

Aim:   The aim of the present was to Study of apoptotic changes of caspase 3 and 9 proteins and cytochrome c of heart tissue after High intensity interval training (HIIT) and thyme honey in type 2 diabetic rats. Method: The statistical population consisted of male diabetic rats that 20 rats were divided into 4 groups: control, HIIT, thyme honey, HIIT-thyme honey. The training protocol was implemented as eight weeks of HIIT and five sessions per week. Insulin was measured using the insulin kit and the ELISA method and the expression of caspase 3, caspase 9 and cytochrome-C genes by RT-PCR method. Statistical analysis was performed using SPSS version 22 software and two-factor analysis of variance and determination of effect size and Bonferroni post hoc. Results: compared to the control group, HIIT led to a significant decrease in glucose (0.0001) and insulin resistance index (0.001). HIIT increased the expression of caspase 9 genes (0.001) in cardiac cells compared to the control group. Conclusion: It can be concluded that performing HIIT by diabetic patients can lead to an increase in the apoptotic factor. Therefore, the use of more moderated intensities, as well as the use of substances with antioxidant and anti-inflammatory properties, such as honey, along with exercise can be useful.

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This is an open access article distributed under the following Creative Commons license: Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)

References

  1. .Zhang X, Chen C. A new insight of mechanisms, diagnosis and treatment of diabetic cardiomyopathy. Endocrine. 2012;41(3):398-409.
  2. Bockus LB, Humphries KM. cAMP-dependent protein kinase (PKA) signaling is impaired in the diabetic heart. Journal of Biological Chemistry. 2015;290(49):29250-8.
  3. Shiroo A, Salami S, Khadem Ansari M, Ghaderi Pakdel F, Khadem Vatani K, Saadatian R, et al. Protective Effect of Vitamin E on Diabetes Induced Apoptosis and Oxidative Stress in Rat Heart Tissue. Iranian Journal of Endocrinology and Metabolism. 2008;10(1):67-74. [In Persian]
  4. Tanoorsaz S, Behpour N, Tadibi V. Investigating the Effect of mid-term of aerobic exercise on apoptosis biomarkers in the cardiomyocytes of streptozotocin-induced diabetic rats. Journal of Fasa University of Medical Sciences. 2018;7(4):488-97. [In Persian]
  5. Roberts TJ, Burns AT, MacIsaac RJ, MacIsaac AI, Prior DL, La Gerche A. Exercise capacity in diabetes mellitus is predicted by activity status and cardiac size rather than cardiac function: a case control study. Cardiovascular diabetology. 2018;17(1):1-14.
  6. Mazraekhatiri M, Arshadi S, Banaeifar A, Abednatanzi H. The effect of eight weeks of high-intensity interval training and thyme honey on changes in Pdx1 gene expression in pancreatic tissue and insulin resistance index male rats with type 2 diabetes. EBNESINA. 2022;24(1):4-15. [In Persian]
  7. Erejuwa OO, Sulaiman SA, Wahab MSA. Fructose might contribute to the hypoglycemic effect of honey. Molecules. 2012;17(2):1900-15.
  8. Al-Waili N. Intrapulmonary administration of natural honey solution, hyperosmolar dextrose or hypoosmolar distill water to normal individuals and to patients with type-2 diabetes mellitus or hypertension: their effects on blood glucose level, plasma insulin and C-peptide, blood pressure and peaked expiratory flow rate. European journal of medical research. 2003;8(7):295-303.
  9. Erejuwa OO, Sulaiman SA, Ab Wahab MS. Honey: a novel antioxidant. Molecules. 2012;17(4):4400-23.
  10. Mehri A. Effect of 8 Weeks Aerobic Training and Supplementation of Resveratrol on Oxidative Marker MDA and Antioxidant SOD and GPX Cardiomyocytes Tissue in Streptozotocin-Diabetic Rats. 2020.
  11. Mehran M, Hoseini H, Hatami A, Taghizade M. Investigation of components of seven Species of Thyme Essential oils and comparison of their antioxidant properties. Journal of Medicinal Plants. 2016;15(58):134-40.
  12. Imman Shahidi M HH. Animal models of diabetes. Iranian Journal of Diabetes and Lipid 2002;2:1-10.
  13. Erejuwa OO, Sulaiman SA, Wahab MSA, Sirajudeen KNS, Salleh MSM, Gurtu S. Antioxidant protective effect of glibenclamide and metformin in combination with honey in pancreas of streptozotocin-induced diabetic rats. International journal of molecular sciences. 2010;11(5):2056-66.
  14. Erejuwa OO, Sulaiman SA, Wahab MSA, Sirajudeen KN, Salleh MSM, Gurtu S. Differential responses to blood pressure and oxidative stress in streptozotocin-induced diabetic wistar-kyoto rats and spontaneously hypertensive rats: effects of antioxidant (Honey) treatment. International Journal of Molecular 2011;12(3):1888-907.
  15. Rodrigues B, Figueroa DM, Mostarda CT, Heeren MV, Irigoyen M-C, De Angelis K. Maximal exercise test is a useful method for physical capacity and oxygen consumption determination in streptozotocin-diabetic rats. Cardiovascular diabetology. 2007;6(1):1-7.
  16. Bedford TG, Tipton CM, Wilson NC, Oppliger RA, Gisolfi CV. Maximum oxygen consumption of rats and its changes with various experimental procedures. Journal of Applied Physiology. 1979;47(6):1278-83.
  17. PITHON-CURI TNC. Aprogram Of Moderate Physical Training For Wistar Rats Based On Maximal Oxygen Consumption. Journal of strength and conditioning research. 2007;21(3):000-.
  18. Høydal MA, Wisløff U, Kemi OJ, Ellingsen Ø. Running speed and maximal oxygen uptake in rats and mice: practical implications for exercise training. European Journal of Preventive Cardiology. 2007;14(6):753-60.
  19. Thomas C, Bishop D, Moore-Morris T, Mercier J. Effects of high-intensity training on MCT1, MCT4, and NBC expressions in rat skeletal muscles: influence of chronic metabolic alkalosis. American Journal of Physiology-Endocrinology and Metabolism. 2007;293(4):E916-E22.
  20. Akbarzadeh A. The effect of high intencity interval training combined with curcumin supplementation on Plasma glucose concentration and insulin resistance in diabetic rats. SSU_Journals. 2018;25(12):961-9.
  21. Rezaei R, Norshahi M, Bigdeli M, Khodagholi F, Haghparast A. Effect of eight weeks of continuous and interval intense aerobic exercise on VEGFR-2 and VEGF-A values in the brain tissue of wistar rats. Journal of Exercise Physiology and Physical Activity. 2015;8(2):1213-21.
  22. Yeylaghi Ashrafi MR, Abednatanzi H, Ghazalian F. The effect of eight weeks of high intensity interval training and n-chromosomal royal jelly on G6Pase gene expression in hepatocytes, glucose levels and insulin resistance in type 2 diabetic rats. Razi Journal of Medical Sciences. 2020;27(10):135-50.
  23. Tokmakidis SP, Zois CE, Volaklis KA, Kotsa K, Touvra A-M. The effects of a combined strength and aerobic exercise program on glucose control and insulin action in women with type 2 diabetes. European journal of applied physiology. 2004;92(4):437-42.
  24. Yousefipoor P, Tadibi V, Behpoor N, Parnow A, Delbari M, Rashidi S. Effects of aerobic exercise on glycemic control and risk factors CVD in people with type 2 diabetes. Med J Mashhad Univ Med Sci. 2015;57(9):976-84. [In Persian]
  25. Jorge MLMP, de Oliveira VN, Resende NM, Paraiso LF, Calixto A, Diniz ALD, et al. The effects of aerobic, resistance, and combined exercise on metabolic control, inflammatory markers, adipocytokines, and muscle insulin signaling in patients with type 2 diabetes mellitus. Metabolism. 2011;60(9):1244-52.
  26. Richard D, Wendy K, Richard K, Eugene J, Eric C, Barbara A. Critical review dietary carbohydrate restriction as the first approach in diabetes management: Critical review and evidence base. Nutrition. 2015;31(9):1-13.
  27. Bello AI, Owusu-Boakye E, Adegoke BO, Adjei DN. Effects of aerobic exercise on selected physiological parameters and quality of life in patients with type 2 diabetes mellitus. International journal of general medicine. 2011;4:723.
  28. Andreotti DZ, Silva JdN, Matumoto AM, Orellana AM, De Mello PS, Kawamoto EM. Effects of physical exercise on autophagy and apoptosis in aged brain: Human and animal studies. Frontiers in Nutrition. 2020;7:94.
  29. Chen K-C, Peng C-C, Hsieh C-L, Peng RY. Exercise ameliorates renal cell apoptosis in chronic kidney disease by intervening in the intrinsic and the extrinsic apoptotic pathways in a rat model. Evidence-Based Complementary and Alternative Medicine. 2013;2013.
  30. Kwak HB, Song W, Lawler JM. Exercise training attenuates age‐induced elevation in Bax/Bcl‐2 ratio, apoptosis, and remodeling in the rat heart. The FASEB Journal. 2006;20(6):791-3.
  31. French JP, Hamilton KL, Quindry JC, Lee Y, Upchurch PA, Powers SK. Exercise‐induced protection against myocardial apoptosis and necrosis: MnSOD, calcium‐handling proteins, and calpain. The FASEB Journal. 2008;22(8):2862-71.
  32. Zarali M, Etemad Z, Azizbeigi K, Karimi P. Effect of 8 Weeks of High Intensity Interval Training (HIIT) With and Without Calorie Restriction on Gene Expressio
  33. n of Caspase-3 and Caspase-9 Proteins in Male Rats. Journal of Arak University of Medical Sciences. 220;(3):13-30. [In Persian]
  34. Siahkohian M, Asgharpour-Arshad M, Bolboli L, Jafari A, Hesari FS. Effect of 12-weeks aerobic training on some indices of skeletal muscle apoptosis in male rats. Medical Journal of Tabriz University of Medical Sciences. 2017;39(6):35-43. [In Persian]
  35. Rastogi RP RR, Sinha RP. Apoptosis: Molecular mechanisms and pathogenicity. EXCLI J. 2009;8:155-88.
  36. Marzetti E, Privitera G, Simili V, Wohlgemuth SE, Aulisa L, Pahor M, et al. Multiple pathways to the same end: mechanisms of myonuclear apoptosis in sarcopenia of aging. TheScientificWorldJOURNAL. 2010;10:340-9.
  37. Peterson JM, Bryner RW, Sindler A, Frisbee JC, Alway SE. Mitochondrial apoptotic signaling is elevated in cardiac but not skeletal muscle in the obese Zucker rat and is reduced with aerobic exercise. Journal of applied physiology. 2008;105(6):1934-43.
  38. Javid Tabrizi N, Bashiri J, Narimani Rad M. Effect of 12 Weeks of Treadmill Aerobic Training on Cytochrome c and Caspase-9 gene Expression in Cardiac Muscle of Male Rats. Qom University of Medical Sciences Journal. 2017;11(6):1-9. [In Persian]
  39. Ho T-J, Huang C-C, Huang C-Y, Lin W-T. Fasudil, a Rho-kinase inhibitor, protects against excessive endurance exercise training-induced cardiac hypertrophy, apoptosis and fibrosis in rats. European journal of applied physiology. 2012;112(8):2943-55.
  40. Song W, Kwak H-B, Lawler JM. Exercise training attenuates age-induced changes in apoptotic signaling in rat skeletal muscle. Antioxidants & redox signaling. 2006;8(3-4):517-28.
  41. Koçtürk S, Kayatekin B, Resmi H, Açıkgöz O, Kaynak C, Özer E. The apoptotic response to strenuous exercise of the gastrocnemius and solues muscle fibers in rats. European journal of applied physiology. 2008;102(5):515-24.
  42. Safar nezhad A, peeri M, Azarbayjani MA, Delfan M. Effect of 8 Weeks High Intensity Interval Training on the Gene Expression of BAX and BCL-2 in the Left Ventricle of Diabetic Male Wistar Rats. J Shahid Sadoughi Uni Med Sci 2020; 28(7): 2833-43. [In Persian]
Journal of Applied Health Studies in Sport Physiology
Volume 9, Issue 2
August 2022
Pages 23-36

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History

  • Receive Date: 28 June 2022
  • Revise Date: 12 August 2022
  • Accept Date: 04 September 2022

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