Effect of Resistance Training with Milk Protein Concentrate (MPC) Supplementation on serum levels of Follistatin and myostatin and Muscle Hypertrophy in Beginner Bodybuilders

Document Type : Research Paper I Open Access I Released under (CC BY-NC 4.0) license

Authors

1 MSc in Exercise Physiology, Department of Sport Sciences, Faculty of Educational Sciences and Psychology, Azarbaijan Shahid Madani University, Tabriz, Iran.

2 Assistant Professor, Department of Sport Sciences, Faculty of Educational Sciences and Psychology, Azarbaijan Shahid Madani University, Tabriz, Iran.

Abstract

Aim: The use of resistance training along with dietary supplements are the most widely used methods that improve strength and increase muscle hypertrophy and improve performance in athletes. The aim of this study was to evaluate the effect of resistance training with milk protein concentrate supplementation on serum follistatin and myostatin levels and muscle hypertrophy in novice bodybuilders. Methods: The present study is a quasi-experimental study and its statistical population consisted of beginner bodybuilders aged 20 to 35 years who participated in this study as 40 subjects. Subjects were randomly divided into four groups of exercise - supplement, exercise - Placebo, supplement and control were divided. Subjects from both training groups participated in an eight-week resistance training program three sessions per week. The supplement groups consumed a 30-gram pack of milk protein concentrate daily for eight weeks between lunch and dinner meals and immediately after exercise. Data were compared using repeated measures analysis of variance and correlated t-test. Results:  The results showed that resistance training with and without supplementation led to a significant increase in serum folate levels (p = 0.001), muscle volume (p = 0.001) and muscle strength (p = 0.001) and a significant decrease. Serum myostatin levels (p = 0.002) were higher than the control group. The results also showed that supplementation of milk protein concentrate without exercise had no significant effect on serum folate and myostatin levels, muscle volume and muscle strength in novice bodybuilders (P <0.05). Conclusion: Considering the significant effect of resistance training on hypertrophy factors, it can be concluded that resistance training with milk protein supplementation for eight weeks can affect the acquisition of strength and muscle mass of novice bodybuilders.

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This is an open access article distributed under the following Creative Commons license: Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)

Refrence
 
1.             Waldron, J., et al., Concurrent creatine monohydrate supplementation and resistance training does not affect markers of hepatic function in trained weightlifters. Journal of Exercise Physiology Online, 2002. 5(1).
2.             Nasiri Hooshmand M, Hosseini Kakhk SA. Comparison of the effects of three training programs of stretch-resistance, elastic-resistance and plyometric-resistance on muscle function and body composition in male bodybuilders. Journal of Applied Exercise Physiology. 2019 Jan 21;14(28):71-88. [In Persian].
3.             Schiaffino, S., et al., Mechanisms regulating skeletal muscle growth and atrophy. The FEBS journal, 2013. 280(17): p. 4294-4314.
4.             McCroskery, S., et al., Myostatin negatively regulates satellite cell activation and self-renewal. Journal of Cell Biology, 2003. 162(6): p. 1135-1147.
5.             Lee, S.-J., Quadrupling muscle mass in mice by targeting TGF-ß signaling pathways. PloS one, 2007. 2(8): p. e789.
6.             Hansen, J., et al., Exercise induces a marked increase in plasma follistatin: evidence that follistatin is a contraction-induced hepatokine. Endocrinology, 2011. 152(1): p. 164-171.
7.             Estes, R.R., et al., The effect of high intensity interval run training on cross-sectional area of the vastus lateralis in untrained college students. International journal of exercise science, 2017. 10(1): p. 137.
8.             Farzanegi P, Zamani M, Khalili A, Dehghani H, Fotohi R, Ghanbarpour MR, Hosseini SA, Peeri M, Rahmati-Ahmadabad S, Azarbayjani MA. Effects of upper-and lower-extremity resistance training on serum vascular endothelial growth factor, myostatin, endostatin and follistatin levels in sedentary male students. Science & Sports. 2021 Apr 1;36(2):139-e1.
9.            Faezi, G., et al., The effect of 4 weeks high-intensity interval training (HIIT) on the content of downstream and upstream mTORC1 pathways gastrocnemius muscle of type 2 diabetic rats. Medical Science Journal of Islamic Azad Univesity-Tehran Medical Branch, 2020. 30(2): p. 120-127. [In Persian]
10.          Nabuco, H.C., et al., Effects of pre‐or post‐exercise whey protein supplementation on oxidative stress and antioxidant enzymes in older women. Scandinavian Journal of Medicine & Science in Sports, 2019. 29(8): p. 1101-1108.
11.          DeNysschen, C.A., et al., Resistance training with soy vs whey protein supplements in hyperlipidemic males. Journal of the International Society of Sports Nutrition, 2009. 6(1): p. 1-9.
12.          Delecluse, C., R. Diels, and M. Goris, Effect of creatine supplementation on intermittent sprint running performance in highly trained athletes. Journal of strength and conditioning research, 2003. 17(3): p. 446-454.
13.          Aimutis, W.R., Bioactive properties of milk proteins with particular focus on anticariogenesis. The Journal of nutrition, 2004. 134(4): p. 989S-995S.
14.          Calvello, R., et al., Bovine and soybean milk bioactive compounds: Effects on inflammatory response of human intestinal Caco-2 cells. Food Chemistry, 2016. 210: p. 276-285.
15.          Gad, A.S., et al., Antioxidant activity and hepatoprotective effects of whey protein and Spirulina in rats. Nutrition, 2011. 27(5): p. 582-589.
16.          Traverso, N., et al., Oxidative stress in the animal model: the possible protective role of milk serum protein. Mediterranean Journal of Nutrition and Metabolism, 2010. 3(2): p. 173-178.
17.          Ha, E. and M.B. Zemel, Functional properties of whey, whey components, and essential amino acids: mechanisms underlying health benefits for active people. The Journal of nutritional biochemistry, 2003. 14(5): p. 251-258.
18.          Seyedi, A. and S.R. Attarzadeh Hosseini, Effects of one session of progressive training after whey protein consumption on the mfo, fatmax, and insulin resistance in overweight women. The Iranian Journal of Obstetrics, Gynecology and Infertility, 2018. 21(1): p. 60-69.
19.          Keri Marshall, N., Therapeutic applications of whey protein. Alternative medicine review, 2004. 9(2): p. 136-156.
20.          Haff, G.G. and C. Dumke, Laboratory manual for exercise physiology. 2021: Human Kinetics.
21.          Jensky, N.E., et al., Exercise does not influence myostatin and follistatin mRNA expression in young women. Journal of strength and conditioning research/National Strength & Conditioning Association, 2010. 24(2): p. 522.
22.          Figueroa, A., et al., Influence of L-citrulline and watermelon supplementation on vascular function and exercise performance. Current opinion in clinical nutrition and metabolic care, 2017. 20(1): p. 92-98.
23.          Ahmadi Kani Golzar F, Sheikholeslami Vatani D, Kashkooli V, Moradi H, Farhangian M. The effects of whey protein isolate supplement and strength training on weight loss, body composition, strength and muscle hypertrophy in overweight young men. Iranian Journal of Nutrition Sciences & Food Technology. 2012;7(2):37-46.
24.          Diel, P., et al., Analysis of the effects of androgens and training on myostatin propeptide and follistatin concentrations in blood and skeletal muscle using highly sensitive immuno PCR. Molecular and cellular endocrinology, 2010. 330(1-2): p. 1-9.
25.          Sâmpelean, D., et al., The prognosis of glycoregulation disturbances and insulin secretion in alcoholic and C virus liver cirrhosis. Romanian journal of internal medicine= Revue roumaine de medecine interne, 2009. 47(4): p. 387-392.
26.          Aghda, A.K., et al., Evaluation of military optimal performance challenge (mopc) test in military students at a training center, Tehran, Iran. Journal of Military Medicine, 2018. 20(2): p. 181-188.
27.          Motevalli, M.S., et al., The effect of rate of weight reduction on serum myostatin and follistatin concentrations in competitive wrestlers. International journal of sports physiology and performance, 2015. 10(2): p. 139-146. [In Persian].
28.          Motevalli MS, Dalbo VJ, Attarzadeh RS, Rashidlamir A, Tucker PS, Scanlan AT. The effect of rate of weight reduction on serum myostatin and follistatin concentrations in competitive wrestlers. International journal of sports physiology and performance. 2015 Mar 1;10(2):139-46. [In Persian].
29.          Allen, D.L., D.S. Hittel, and A.C. McPherron, Expression and function of myostatin in obesity, diabetes, and exercise adaptation. Medicine and science in sports and exercise, 2011. 43(10): p. 1828.
30.          Schoenfeld, B.J., The mechanisms of muscle hypertrophy and their application to resistance training. The Journal of Strength & Conditioning Research, 2010. 24(10): p. 2857-2872.
31.          Marx, J.O., et al., Low-volume circuit versus high-volume periodized resistance training in women. Medicine & Science in Sports & Exercise, 2001. 33(4): p. 635-643.
32.          Sanborn, K., et al., Short-term performance effects of weight training with multiple sets not to failure vs. a single set to failure in women. The Journal of Strength & Conditioning Research, 2000. 14(3): p. 328-331.
33.          Wolfe, B.L., L.M. Lemura, and P.J. Cole, Quantitative analysis of single-vs. multiple-set programs in resistance training. The Journal of Strength & Conditioning Research, 2004. 18(1): p. 35-47.
34.          Otto, R.M. and R.N. Carpinelli, A CRITICAL ANALYSIS OF THE SINGLE VERSUS MULTIPLE SET DEBATE. Journal of Exercise Physiology Online, 2006. 9(1).
35.          Paulsen, G., D. Myklestad, and T. Raastad, The influence of volume of exercise on early adaptations to strength training. The Journal of Strength & Conditioning Research, 2003. 17(1): p. 115-120.
36.          Englund, D.A., et al., Nutritional supplementation with physical activity improves muscle composition in mobility-limited older adults, the VIVE2 study: a randomized, double-blind, placebo-controlled trial. The Journals of Gerontology: Series A, 2018. 73(1): p. 95-101.
37.          Rı́os, R., et al., Myostatin is an inhibitor of myogenic differentiation. American Journal of Physiology-Cell Physiology, 2002. 282(5): p. C993-C999.
38.          Attarzadeh Hosseini, S.R., N. Moeinnia, and M. Motahari Rad, The effect of two intensities resistance training on muscle growth regulatory myokines in sedentary young women. Obesity Medicine, 2017. 5. [In Persian].
39.          Latres, E., et al., Myostatin blockade with a fully human monoclonal antibody induces muscle hypertrophy and reverses muscle atrophy in young and aged mice. Skeletal Muscle, 2015. 5(1): p. 1-13.
40.          Lee, S.-J. and A.C. McPherron, Regulation of myostatin activity and muscle growth. Proceedings of the National Academy of Sciences, 2001. 98(16): p. 9306-9311.
41.          Amthor, H., et al., Muscle hypertrophy driven by myostatin blockade does not require stem/precursor-cell activity. Proceedings of the National Academy of Sciences, 2009. 106(18): p. 7479-7484.
42.          BORST, S.E., et al., Effects of resistance training on insulin-like growth factor-I and IGF binding proteins. Medicine & Science in Sports & Exercise, 2001. 33(4): p. 648-653.
43.          Bohannon, R.W., Considerations and practical options for measuring muscle strength: a narrative review. BioMed Research International, 2019. 2019.
44.          Yang, N.-P., et al., Relationship between muscle strength and fall episodes among the elderly: the Yilan study, Taiwan. BMC geriatrics, 2018. 18(1): p. 1-7.
45.          Gad, A.S., Use of skim milk after resistance training in young men, novices, male weightlifters. American Journal of Nutrition College, 2010. 1(1): p. 45-63.
46.          Haruna, I.L., et al., Associations between the bovine Myostatin gene and milk fatty acid composition in New Zealand Holstein-Friesian× Jersey-cross cows. Animals, 2020. 10(9): p. 1447.
47.          Bagheri L, Faramarzi M, Bani Talebi E, Azimiyan A. The effect of sequence order of combined training (strength and endurance) on Myostatin, Follistatin and Follistatin/Myostatin ratio in older women. Sport Physiology. 2015 Aug 23;7(26):143-64.
48.          Ahtiainen JP. Physiological and molecular adaptations to strength training. InConcurrent Aerobic and Strength Training 2019 (pp. 51-73). Springer, Cham.
49.          Kraemer, W.J., et al., American College of Sports Medicine position stand. Progression models in resistance training for healthy adults. Medicine and science in sports and exercise, 2002. 34(2): p. 364-380.
50.          Humburg, H., et al., 1-set vs. 3-set resistance training: a crossover study. Journal of Strength and Conditioning Research, 2007. 21(2): p. 578.
51.          Williams, M., Dietary supplements and sports performance: herbals. Journal of the international society of sports nutrition, 2006. 3(1): p. 1-6.