Effects of 8 weeks of continuous and intermittent aerobic training with resveratrol supplementation on Beclin-1 and LC3I-II genes expression in heart tissue of diabetic male rats

Document Type : Research Paper I Open Access I Released under (CC BY-NC 4.0) license

Authors

1 Assistant Prof., Dept. of Exercise Physiology, Payame Noor University, Tehran, Iran.

2 Instructor, Dept. of Exercise Physiology, Payame Noor University. Tehran Iran.

3 Dept of Physical Education and Sport Sciences, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan Iran.

Abstract

Aim: Diabetic cardiomyopathy is a complication of type 2 diabetes that can lead to myocardial cell autophagy through Beclin-1 and light chain 3 proteins. The aim of this study was to evaluate the effects of 8 weeks of continuous and intermittent aerobic exercise with resveratrol supplementation on Beclin-1 and LC3 gene expression in the heart tissue of diabetic male rats. Methods:  For this purpose, 56 male Wistar rats with mean (age 40-50 weeks and weight 430-373 g) were randomly divided into 8 groups (n = 7) control (C), diabetic (D), saline (S), Resveratrol (Sup), Continuous exercise (E), Interval exercise (HIIT), Continuous exercise/Supplement (E+Sup), Intervalt exercise/Resveratrol (HIIT+Sup).  Diabetes was diagnosed by intraperitoneal injection of streptozotocin at a dose of 50 mg / kg body weight. Continuous training rats trained three days a week for 8 weeks at a speed of 15 meters per minute.  The intermittent training protocol also included 10 one-minute sets with an intensity of 50% vo2max on the treadmill and two minutes of rest between sets, starting at 14 meters per minute and increasing rapidly by two meters per minute each week.  Data were analyzed using two-way analysis of variance at the α (α ≥0.05) level. Results:  The results showed that Beclin-1 and LC3 in the diabetic induction group had a significant decrease compared to the healthy control group (P = 0.001). Compared with the diabetic group, the diabetic + exercise + supplement group showed a significant increase in Bcline-1 and LC3 (P = 0.001). Conclusion:   Exercise combined with resveratrol supplementation can increase Beclin-1 and LC3 autophagy factors” which inhibit apoptosis and reduce heart tissue damage in diabetics.

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This is an open access article distributed under the following Creative Commons license: Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)

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Journal of Applied Health Studies in Sport Physiology
Volume 8, Issue 2
August 2021
Pages 61-69

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History

  • Receive Date: 02 October 2021
  • Revise Date: 15 November 2021
  • Accept Date: 24 November 2021

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