The Combined Effect of Aerobic Exercise with Saffron Extract on Some Indices of Soleus Muscle Apoptosis Regulatory Factors of Male Rats Following an Aerobic Exercise Until Exhaustion

Document Type : Research Paper I Open Access I Released under (CC BY-NC 4.0) license

Author

Assistant Professor، Department of Physical Education and Exercise Science، Faculty of Humanities Ayatollah Ozma Borujerdi University, Borujerd، Iran

Abstract

Aim: Saffron has high levels of antioxidant compounds are able to Inhibition of apoptosis induced by exercise to exhaustion. The present study aims to investigate the effect of the combined effect of aerobic exercise with Saffron extract on some indices of soleus muscle apoptosis regulatory factors of male rats following an aerobic exercise until exhaustion. Methods: 48 Wistar male rats were assigned into following four groups (N=10): G1) placebo; without exercise: G2) supplement; without exercise+ Saffron extract, 100 mg/kg :G3) exercise; 8 weeks exercise: G4) combination; 8 weeks exercise + Saffron extract, 100 mg/kg. The end of experiment, half of the rats was killed immediately before exhaustive exercise; on the other hand, remaining rats were killed immediately after performing an acute bout of exhaustive exercise on the treadmill. Bax and Bcl2 genes expression were analyzed through the Real Time-Polymerase Chain Reaction (RT-PCR). The data were analyzed using a one-way ANOVA, with the significance level of P < 0.05. Results: The results showed that prior exhaustive bcl-2 gene expression and Bax/Bcl2 ratio of the G3 and G4r groups were significantly mor and lower than the other groups respectively (P < 0.05). As well as, in the all groups except G4, the bax and bcl-2 protein expression increased and decreased, respectively while bax/bcl-2 ratio increased following an exhausting exercise (P < 0.05). Conclusion: In general, 8 weeks aerobic training only and combinational, with use of saffron extract significant increase bcl-2 expression in soleus muscle of rats. As well as the use of saffron extract with aerobic exercise prevented the increase of Bax gene expression and Bcl-2/Bax ratio following an exhausting exercise.

Keywords

References

 
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Journal of Applied Health Studies in Sport Physiology
Volume 6, Issue 2
August 2019
Pages 86-95

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History

  • Receive Date: 29 June 2019
  • Revise Date: 16 March 2020
  • Accept Date: 16 March 2020

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