The effect of Gender on p53 Levels After Downhill Running in Non-Athletes

Document Type : Research Paper I Open Access I Released under (CC BY-NC 4.0) license

Authors

1 Assistant Professor, Department of Physical Education, Marivan branch, Islamic Azad University, Marivan, Iran

2 MSc of Exercise Physiology, Department of Physical Education, Marivan branch, Islamic Azad University, Marivan, Iran

Abstract

Aim: The effect of gender on related-exercise apoptosis is unclear. The aim of this study was to investigate the effect of gender on the p53 level, as apoptosis marker, after downhill running in non-athletes. Methods: Eighteen individuals between the ages of 18 and 30 years were randomly selected and divided into 9 groups (9 males and 9 females). Subjects performed downhill running (12% incline) at 70% of HRmax for 40 min and blood samples were taken before exercise and immidatly after exercise. Blood samples were assayed for serum level of p53. Results:The results showed that p53 was significantly increased after running downhill compared to pre-test in both male and female groups, but this increase was not significantly different between the two groups of men and women. Conclusions: Although more research is needed, we showed that gender was not found to play an important role in apoptosis response to downhill running in non-athletes.

Keywords

References

 
1. Neto, J.C.R., et al., Exhaustive exercise causes an anti-inflammatory effect in skeletal muscle and a pro-inflammatory effect in adipose tissue in rats. European Journal of Applied Physiology, 2009. 106(5): p. 697-704.
2. Soliman, S., A.T. , and S.B. , Effect of six weeks aerobic training accompanied by dietary supplementation of spirulina onOxidative stress index in obese inactive men followed by one session exhaustive exercise. Journal of Applied Health Studies in Sport Physiology, 2018. 5(2): p. 36-44.
3. Phaneuf, S. and C. Leeuwenburgh, Apoptosis and exercise. Medicine and Science in Sports and Exercise, 2001. 33(3): p. 393-396.
4. Ali, M. and Z.F. , The effect of combined supplementation with Methyl Sulfonyl Methane and GLUCOSAMINE on Superoxide dismutase and Malondialdehyde levels after a bout of plyometric exercise training in athlete males. Journal of Applied Health Studies in Sport Physiology, 2019. 5(1): p. 37-44.
5. Krüger, K. and F.C. Mooren, Exercise-induced leukocyte apoptosis. Exercise Immunology Review, 2014. 20: p. 117-134.
6. Samad Safarzadeh Gargari, H.M.H., Mohammad Ali Azarbayjani, Effects of continuous exercise training in accompany with h2o2 injection on male rat cardiac Bax, Bcl-2 level and Bax/BCL-2 Ratio. Journal of Applied Health Studies in Sport Physiology, 2019. 5(2): p. 13-19.
7. Wang, Z.-B., Y.-Q. Liu, and Y.-F. Cui, Pathways to caspase activation. Cell Biology International, 2005. 29(7): p. 489-496.
8. Cooper, D., The Balance between Life and Death: Defining a Role for Apoptosis in Aging. Journal of Clinical and Experimental Pathology, 2012. 4: p. 2161-0681.
9. Sõti, C., A.S. Sreedhar, and P. Csermely, Apoptosis, necrosis and cellular senescence: chaperone occupancy as a potential switch. Aging Cell, 2003. 2(1): p. 39-45.
10.Attardi, L.D., The role of p53-mediated apoptosis as a crucial anti-tumor response to genomic instability: lessons from mouse models. Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis, 2005. 569(1): p. 145-157.
11.Dincer, Y., et al., Circulating p53 and cytochrome c levels in acute myocardial infarction patients. Journal of Thrombosis and Thrombolysis, 2010. 29(1): p. 41-45.
12.Podhorska-OkoĊ‚ów, M., et al., Exercise-induced apoptosis in renal tubular cells of the rat. Folia morphologica, 2004. 63(2): p. 213-216.
13.Kwak, H.-B., Effects of aging and exercise training on apoptosis in the heart. Journal of Exercise Rehabilitation, 2013. 9(2): p. 212-219.
14.Sharafi, H. and R. Rahimi, The effect of resistance exercise on p53, caspase-9, and caspase-3 in trained and untrained men. The Journal of Strength & Conditioning Research, 2012. 26(4): p. 1142-1148.
15.Boroujerdi, S. and R. Rahimi, The apoptotic response to resistance exercise with different intensities in athletes. Medcina dello Sport, 2011. 64(1): p. 31-44.
16.Faraji, H., et al., Apoptosis response to different rest periods after resistance exercise in athletes. Medicina dello Sport 2016. 69(2): p. 173-83.
17.Piotrowska, K., et al., Gender Differences in Response to Prolonged Every-Other-Day Feeding on the Proliferation and Apoptosis of Hepatocytes in Mice. Nutrients, 2016. 8(3): p. 176.
18.Rahimi, R., et al., Effects of creatine monohydrate supplementation on exercise-induced apoptosis in athletes: A randomized, double-blind, and placebo-controlled study. Journal of Research in Medical Sciences: The Official Journal of Isfahan University of Medical Sciences, 2015. 20(8): p. 733.
19.Sharafi, H. and R. Rahimi, The effect of resistance exercise on p53, caspase-9, and caspase-3 in trained and untrained men. J Strength Cond Res, 2012. 26(4): p. 1142-8.
20.Kerksick, C., A. Harvey, and D. Willoughby, Gender-related differences in muscle injury, oxidative stress, and apoptosis. Medicine and science in sports and exercise, 2008. 40(10): p. 1772-1780.
21.Navalta, J.W., et al., Neither gender nor menstrual cycle phase influences exercise-induced lymphocyte apoptosis in untrained subjects. Applied Physiology, Nutrition, and Metabolism, 2007. 32(3): p. 481-486.
22.Kander, M.C., Y. Cui, and Z. Liu, Gender difference in oxidative stress: a new look at the mechanisms for cardiovascular diseases. Journal of cellular and molecular medicine, 2017. 21(5): p. 1024-1032.
23.Jog, N. and R. Caricchio, Differential regulation of cell death programs in males and females by Poly (ADP-Ribose) Polymerase-1 and 17βestradiol. Cell death & disease, 2013. 4(8): p. e758.
24.Verzola, D., et al., Testosterone promotes apoptotic damage in human renal tubular cells. Kidney international, 2004. 65(4): p. 1252-1261.
25.Paroo, Z., E.S. Dipchand, and E.G. Noble, Estrogen attenuates postexercise HSP70 expression in skeletal muscle. American Journal of Physiology-Cell Physiology, 2002. 282(2): p. C245-C251.
26.Fisker, S., et al., Impact of gender and androgen status on IGF-I levels in normal and GH-deficient adults. European journal of endocrinology, 1999. 141(6): p. 601-608.
27.Siu, P.M., et al., Aging influences cellular and molecular responses of apoptosis to skeletal muscle unloading. American Journal of Physiology-Cell Physiology, 2005. 288(2): p. C338-C349.
28.Pollock, M.L. and A.S. Jackson, Research progress in validation of clinical methods of assessing body composition. Medicine and Science in Sports and Exercise, 1984. 16(6): p. 606-615.
29.Villalba, J.M., et al., The influence of dietary fat source on liver and skeletal muscle mitochondrial modifications and lifespan changes in calorie-restricted mice. Biogerontology, 2015. 16(5): p. 655-670.
30. Thompson HJ, Zhu Z, Jiang W. Identification of the apoptosis activation cascade induced in mammary carcinomas by energy restriction. Cancer research. 2004.15;64(4):1541-5.
 
 
 
 
Journal of Applied Health Studies in Sport Physiology
Volume 7, Issue 1
April 2020
Pages 38-44

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History

  • Receive Date: 04 February 2020
  • Revise Date: 07 March 2020
  • Accept Date: 05 April 2020

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