The Effect of Ten Weeks of High Intensity Interval Training and Continuous Endurance Training on The Expression of MIR-205 and VEGF Gene in Mice with Breast Cancer Has Been Compared

Document Type : Research Paper I Open Access I Released under (CC BY-NC 4.0) license

Authors

1 Msc of Exercise Physiology, Department of Sport Physiology, Faculty of Sport Sciences, Shahid Chamran University of Ahvaz, Ahvaz, Iran

2 Associate Professor of Exercise Physiology, Department of Sport Physiology, Faculty of Sport Sciences, Shahid Chamran University of Ahvaz, Ahvaz, Iran

3 Associate Professor of Exercise Physiology, Department of Sport Physiology, Faculty of Sport Sciences, Shahid Chamran University of Ahvaz, Ahvaz, Iran.

Abstract

Aim : Many genes are involved in the development of breast cancer. One is the VEGF (Vascular Endothelial Growth Factor) gene, one of the most important angiogenesis factors. On the other hand, one of the suppressive microRNAs is MIR-205, which is significantly less translatable in breast tumors compared to healthy matched tissue. Therefore, in this study, we compared the effect of 10 weeks of high intensity periodic training with continuous endurance training on MIR-205 and the VEGF gene in mice with breast cancer. Methods: The study was performed on 18 Balbci mice, with a mean body mass of 18-20g. The estrogen-dependent MC4-L2 carcinoma cell line was cultured, cultured, and then one million subcutaneous cancer cells were injected into the herb. One week after tumor formation, mice were randomly divided into three groups of 6: Control (C). ), Continuous endurance (E) and interval high intensity (H) were divided. Exercises were performed 5 times a week for 10 weeks. Each session of protocol E was 60-65% vo2max and group H was 90-85% vo2max. Expression of MIR-205 and VEGF was measured by Real-time PCR. Results: The expression level of MIR-205 was significantly in high intensity Interval group (p = 0.000), and in the Continuous endurance group (p = 0.001), than in the control group. The VEGF gene expression was significantly decreased in the in high intensity Interval group (p = 0.003) and in the Continuous endurance group (p = 0.005). Conclusions: Given the incremental effect of in high intensity Interval training and continuous endurance on MIR-205 expression and a decreasing effect on VEGF gene expression in mice with breast cancer, these two types of training may be effective in decreasing tumor growth. Also, according to the results, High intensity interval training due to less time, may be more effective.

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References

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Journal of Applied Health Studies in Sport Physiology
Volume 6, Issue 1
April 2019
Pages 37-44

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History

  • Receive Date: 07 July 2019
  • Revise Date: 02 September 2019
  • Accept Date: 13 September 2019

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