The Response of Wistar rat’s Weight, Serum Anorexic Hormone and Skeletal Muscle Antioxidant Enzyme Activity to Aerobic Training along with L-Arginine Supplementation

Document Type : Research Paper I Open Access I Released under (CC BY-NC 4.0) license

Authors

1 Associate professor, Department of Sport Science, Faculty of Humanities, University of Zanjan, Zanjan ,Iran

2 Master Student in Applied Exercise Physiology , Department of Sport Sciences, Faculty of Humanities, University of Zanjan, Zanjan, Iran.

Abstract

Background‌ & Objective: The purpose of this study, was to investigate the effect of 8weeks incremental aerobic exercise training with L-arginine supplementation on Muscular Enzymatic activity of superoxide dismutase and serum leptine in adult male wistar rat. Materials & Methods:32 mature male Wistar rats, were randomly divided into 4 groups: (control, incremental aerobic training, Incremental aerobic training+L-Arginine and L-Arginine). The training protocol consisted of running on treadmill for 8 weeks (5day/week). Water and food were freely available for samples. In the L-Arginine supplement groups, 4 gr of L-Arginine were used per 100 ml of water. Then,48 hours after the last exercise session and after 8 hours of fasting, blood and tissue samples were collected and the concentration of leptin hormone was measured by ELISA method and the enzyme activity of super-oxide dismutase in the muscle was measured by spectrophotometric method. Data were analyzed using one-way ANOVA and Tukey's post hoc test. Results: The results showed that after 8 weeks of incremental aerobic exercise training with L-Arginine supplementation there was no significant difference in Muscular Enzymatic activity of superoxide dismutase Between groups (P> 0.05). but, serum leptin levels in the exercise with L-Arginine supplementation group significantly increased compared to exercise and L-Arginine groups (P <0.05). Also after 8 weeks, weight change in exercise with L-Arginine supplementation group were significantly lower than control group (P <0.05). Discussion and conclusion: Regarding the effect of L-arginine supplementation with exercise on increasing blood leptin levels, it may‌ be concluded that L-arginine supplementation prevents blood leptin loss and complications in athletes

Keywords

Main Subjects

References

 
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Journal of Applied Health Studies in Sport Physiology
Volume 5, Issue 2
June 2018
Pages 84-94

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History

  • Receive Date: 13 February 2019
  • Revise Date: 26 April 2019
  • Accept Date: 20 May 2019

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