The Effect of High Intensity Interval Training and Endurance Training Along With Jujube Supplement Consumption on the State of Oxidative Stress and Antioxidant Capacities of Testicular Tissue of Immature Male Wistar Rats

Document Type : Research Paper I Open Access I Released under (CC BY-NC 4.0) license

Authors

1 Department of Sport sciences, faculty of humanity, university of Znjan, Zanjan, Iran

2 Department of Sport Sciences, Faculty of Humanity, University of Zanjan, Zanjan, Iran

3 Assistant Professor,Department of Sport Sciences, Faculty of Humanities, University of Zanjan, Zanjan, Iran

Abstract

Aim: The aim of this study was to investigating the effect of high intensity interval training and endurance training along with jujube supplement consumption on the state of oxidative stress and antioxidant capacity of testicular tissue of immature rats. Methods: 72 immature wistar rats with age of 22 days and weight of 60.64 ± 7.475 grams were randomly divided into 9 groups: control, intense interval training (HIT), intense endurance training (ICT), jujube , ICT + jujube, ICT + placebo, HIT training + placebo, HIT training + jujube and placebo. After a week of familiarization, the ICT protocol including running on a  rodent treadmill for 4 weeks (5 sessions/week) and the HIT protocol including 4 weeks (6 sessions/week) were performed. Distilled water gavage was given to the placebo groups, and jujube extract gavage was given to the jujube groups at a dose of 600 mg/kg of body weight, daily after training. The activities of SOD, GPX and CAT enzymes and MDA and TAC levels were measured by spectrophotometry and ELISA kit. Results: In the HIT group, a significant increase in SOD, GPX and TAC was observed after 4 weeks (P=0.001); In the ICT group, the amount of MDA decreased significantly after 4 weeks (P=0.022), while SOD (P=0.006) and TAC (P=0.001) had a significant increase. The amount of antioxidant activity and the level of MDA and TAC did not change significantly in the group of HIT + jujube extract, on the other hand, in the ICT + jujube extract group, only CAT had a significant decrease (P = 0.012). Conclusions:  The results of the present study showed that intense interval and endurance training has reduced oxidative stress and significantly increased antioxidant enzymes in testicular tissue and immature rats. However, due to the few studies done in this field, more research is needed.

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Publisher: Azarbaijan Shahid Madani University          Copyright © The Authors   

   

 

This is an open access article distributed under the following Creative Commons license: Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)

References

  1. Maffulli NJBmb. The growing child in sport. 1992;48(3):561-8.
  2. Hagmar M, Berglund B, Brismar K, Hirschberg ALJCJoSM. Body composition and endocrine profile of male Olympic athletes striving for leanness. 2013;23(3):197-201.
  3. Çakir-Atabek H, Demir S, PinarbaSili RD, Gündüz NJTJoS, Research C. Effects of different resistance training intensity on indices of oxidative stress. 2010;24(9):2491-7.
  4. Gharakhanlou R, Afzalpour ME, Gaeini AA, Rahnama N. Effects of aerobic exercises on the serum paraoxonase arylesterase activity and lipid profile in non-active healthy men. International Journal of Sports Science and Engineering. 2007;1:105-12. [In Persian]
  5. MR YA, V DR. Aerobic and Anaerobic Exercise of the Acute and Chronic and the Selected Markers of Oxidative Stress: A Systematic Review in Human and Animal Studies. Journal of Sabzevar University of Medical Sciences. 2016;22(Special Issue):1126-38.
  6. Shamsi B, Abedi B, Ramezani S. The effect of eight weeks of resistance training with consumption of Tribulus terrestris extract on antioxidant indices of hippocampal tissue in male rats exposed to stanazol. Journal of Applied Health Studies in Sport Physiology. 2022;9(1):48-60. [In Persian]
  7. Afzalpour M, Gharakhanlou R, Gaeini A, Mohebbi H, Hedayati M. Effects of moderate and vigorous aerobic training on enzyme activity arylesterase (ARE) and total anti-oxidation capacity (TAC) in healthy sedentary men. J of Research in exercise science. 2005;3(9):105-23. [In Persian]
  8. Poblete Aro CE, Russell Guzmán JA, Soto Muñoz ME, Villegas González BEJM. Effects of high intensity interval training versus moderate intensity continuous training on the reduction of oxidative stress in type 2 diabetic adult patients: CAT. 2015;15(7): e6212.
  9. Ugras AFJS, sports. Effect of high intensity interval training on elite athletes’ antioxidant status. 2013;28(5):253-9.
  10. Manna I, Jana K, Samanta P. Effect of different intensities of swimming exercise on testicular oxidative stress and reproductive dysfunction in mature male albino Wistar rats. 2004.
  11. Fanaei H, Azizi Y, Khayat S. A Review: Role of oxidative stress in male infertility %J Journal of Fasa University of Medical Sciences. 2013;3(2):93-103.
  12. Saremi A, Changizi Ashtiani S, Shavandi N, Mombeini Ajsb. The Effect of Different Training Intensities On Spermatogenesis And Reproductive Hormones In Obese Rats. 2013;5(17): 81-94. [In Persian]
  13. Jóźków P, Rossato MJAjomsh. The impact of intense exercise on semen quality. 2017;11(3):654-62.
  14. Ribeiro AS, Deminice R, Schoenfeld BJ, Tomeleri CM, Padilha CS, Venturini D, et al. Effect of resistance training systems on oxidative stress in older women. 2017;27(5):439-47.
  15. Henkel RJA. The impact of oxidants on sperm function. 2005;37(6):205-6.
  16. Turner TT, Lysiak JJJJoa. Oxidative stress: a common factor in testicular dysfunction. 2008;29(5):488-98.
  17. Buzadzic B, Vucetic M, Jankovic A, Stancic A, Korac A, Korac B, et al. New insights into male (in) fertility: the importance of NO. British journal of pharmacology. 2015;172(6):1455-67.
  18. Cooper DM, Nemet D, Galassetti PJCoip. Exercise, stress, and inflammation in the growing child: from the bench to the playground. 2004;16(3):286-92.
  19. Paltoglou G, Fatouros IG, Valsamakis G, Schoina M, Avloniti A, Chatzinikolaou A, et al. Antioxidation improves in puberty in normal weight and obese boys, in positive association with exercise-stimulated growth hormone secretion. 2015;78(2):158-64.
  20. Eriksson BO, Gollnick PD, Saltin BJAPS. Muscle metabolism and enzyme activities after training in boys 11–13 years old. 1973;87(4):485-97.
  21. Taati M, Alirezaei M, Meshkatalsadat M, Rasoulian B, Kheradmand A, Neamati S. Antioxidant effects of aqueous fruit extract of Ziziphus jujuba on ethanol-induced oxidative stress in the rat testes. 2011. 12(1): 39-45. [In Persian]
  22. Li J-W, Fan L-P, Ding S-D, Ding X-LJFc. Nutritional composition of five cultivars of Chinese jujube. 2007;103(2):454-60.
  23. Al-Reza SM, Bajpai VK, Kang SC. Antioxidant and antilisterial effect of seed essential oil and organic extracts from Zizyphus jujuba. Food and chemical toxicology. 2009;47(9):2374-80.
  24. Ebrahimi S, Sadeghi H, Pourmahmoudi A, Askariyan S, Askari SJAd. Protective effect of Zizphus Vulgaris extract, on liver toxicity in laboratory rats. 2011;16(2):172-80. [In Persian]
  25. Rafiei S, Bazyar Y, Edalatmanesh MA. Effect of Gallic Acid and Endurance Exercise Training on BDNF in a Model of Hippocampal Degeneration. The Neuroscience Journal of Shefaye Khatam. 2016;4(1):1-6. [In Persian]
  26. Abbaszadeh M, Matinhomaee H, Farzanegi P. The Effect of Anabolic Steroid Boldenone, zizyphus jujube and training on testicular tissue damage in Wistar rats. Journal of Neyshabur University of Medical Sciences. 2019;7(22):144-56. [In Persian]
  27. Bijeh N, Hejazi K, Delpasand AJPR. Acute and Chronic Responses of Serum Leptin Hormone to Different Intensities of Exercise in Rats with Polycystic Ovarian Syndrome. 2015;18(1):95-106. [In Persian]
  28. Rahmani A, Gorzi A, Mohammadi Z. The Effects of Two Models of Training with Different Days of Rest-to-Training Ratios and Natural Honey Supplementation on Serum Concentration of Interleukin-6 and its Hippocampal Gene Expression in Male Immature Rats. Sport Physiology. 2019;11(42):113-32. [In Persian]
  29. Joo Y-I, Sone T, Fukunaga M, Lim S-G, Onodera S. Effects of endurance exercise on three-dimensional trabecular bone microarchitecture in young growing rats. Bone. 2003;33(4): 485-493.
  30. Tehrani BJ, Arefi RG. The effect of eight weeks of aerobic interval training and jujube extract on vascular endothelial growth factor in heart tissue of male Wistar rats with myocardial infarction. Journal of Motion and Behavioral Sciences. 2020., 3(1):1-11. [In Persian]
  31. Yahyaei B, Nouri M, Matmir H. Healing effects of Ziziphus jujuba hydroalcoholic extract with exercise training on histopathological changes of male wistar rats testicular tissue in response to Boldenone steroid administration. Jorjani Biomedicine Journal. 2018;6(1):12-21. [In Persian]
  32. Zhang H, Jiang L, Ye S, Ye Y, Ren F. Systematic evaluation of antioxidant capacities of the ethanolic extract of different tissues of jujube (Ziziphus jujuba Mill.) from China. Food and chemical toxicology. 2010; 48(6): 1461-1465.
  33. Arthur J, Boyne R. Superoxide dismutase and glutathione peroxidase activities in neutrophils from selenium deficient and copper deficient cattle. Life sciences. 1985;36(16):1569-75.
  34. Paglia DE, Valentine WN. Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. The Journal of laboratory and clinical medicine. 1967;70(1):158-69.
  35. Aebi H. [13] Catalase in vitro. Methods in enzymology. 1984;105:121-6.
  36. Miller NJ, Rice-Evans C, Davies MJ, Gopinathan V, Milner A. A novel method for measuring antioxidant capacity and its application to monitoring the antioxidant status in premature neonates. Clinical science. 1993;84(4):407-12.
  37. Poblete Aro CE, Russell Guzmán JA, Soto Munoz ME, Villegas Gonzalez BE. Effects of high intensity interval training versus moderate intensity continuous training on the reduction of oxidative stress in type 2 diabetic adult patients: CAT. Medwave. 2015;15(7):e6212.
  38. Cocks M, Shaw CS, Shepherd SO, Fisher JP, Ranasinghe AM, Barker TA, et al. Sprint interval and endurance training are equally effective in increasing muscle microvascular density and eNOS content in sedentary males. The Journal of physiology. 2013;591(3):641-56.
  39. Majerczak J, Rychlik B, Grzelak A, Grzmil P, Karasinski J, Pierzchalski P, et al. Effect of 5-week moderate intensity endurance training on the oxidative stress, muscle specific uncoupling protein (UPC3) and superoxide dismutase (SOD2) contents in vastus lateralis of young healthy men. Journal of physiology and pharmacology. 2010., 61(6):743-751.
  40. Bogdanis G, Stavrinou P, Fatouros I, Philippou A, Chatzinikolaou A, Draganidis D, et al. Short-term high-intensity interval exercise training attenuates oxidative stress responses and improves antioxidant status in healthy humans. 2013., 61: 171-177.
  41. Cunningham P, Geary M, Harper R, Pendleton A, Stover S. High Intensity Sprint Training Reduces Lipid Peroxidation In Fast-Twitch Skeletal Muscle. Journal of Exercise Physiology Online. 2005;8(6): 150-158.
  42. Chevion S, Moran DS, Heled Y, Shani Y, Regev G, Abbou B, et al. Plasma antioxidant status and cell injury after severe physical exercise. Proceedings of the National Academy of Sciences. 2003;100(9):5119-23.
  43. Arbabian M, Amirzadegan M, Tavalaee M, Nasr-Esfahani M. Oxidative Stress and Its Effects on Male Infertility: A Review Study. Journal of Rafsanjan University of Medical Sciences. 2018;17(3): 253-274. [In Persian]
  44. Fanaei H, Azizi Y, Khayat S. A review: role of oxidative stress in male infertility. Journal of Fasa University of Medical Sciences. 2013;2:93-103. [In Persian]
  45. Manna I, Jana K, Samanta P. Effect of intensive exercise‐induced testicular gametogenic and steroidogenic disorders in mature male Wistar strain rats: a correlative approach to oxidative stress. Acta Physiologica Scandinavica. 2003;178(1):33-40.
  46. Maleki BH, Tartibian B, Mooren FC, Nezhad FY, Yaseri M. Saffron supplementation ameliorates oxidative damage to sperm DNA following a 16-week low-to-intensive cycling training in male road cyclists. Journal of functional foods. 2016;21:153-66. [In Persian]
  47. Safarinejad MR, Azma K, Kolahi AA. The effects of intensive, long-term treadmill running on reproductive hormones, hypothalamus-pituitary-testis axis, and semen quality: a randomized controlled study. The Journal of endocrinology. 2009;200(3):259-71. [In Persian]
  48. Safarinejad MR, Azma K, Kolahi AA. The effects of intensive, long-term treadmill running on reproductive hormones, hypothalamus-pituitary-testis axis, and semen quality: a randomized controlled study. Journal of Endocrinology. 2009;200(3):259. [In Persian]
  49. Nirupama M, Devaki M, Nirupama R, Yajurvedi HJJop, biochemistry. Chronic intermittent stress-induced alterations in the spermatogenesis and antioxidant status of the testis are irreversible in albino rat. 2013;69(1):59-68.
  50. Mombeyni A, Bahmanzade M, Sarami A, Changizi-Ashtiyani S, Parastesh MJJoAUoMS. The Effect of Increasing Resistance Training on Testicular Oxidative Stress and Quality of Spermatogenesis in Male Rats. 2018;21(4):86-97. [In Persian]
  51. Joseph A-M, Nguyen L-D, Welter A, Dominguez J, Behnke B, Adhihetty P. Mitochondrial adaptations evoked with exercise are associated with a reduction in age-induced testicular atrophy in Fischer-344 rats. Biogerontology. 2014;15(5):517-34.
  52. Gebreegziabher Y, Marcos E, McKinon W, Rogers G. Sperm characteristics of endurance trained cyclists. International journal of sports medicine. 2004;25(04):247-51.
  53. Hajizadeh Maleki B, Tartibian B, Eghbali M, Asri‐Rezaei S. Comparison of seminal oxidants and antioxidants in subjects with different levels of physical fitness. Andrology. 2013;1(4):607-14.
  54. Arbabian M, Amirzadegan M, Tavalaee M, Nasr-Esfahani M. Oxidative Stress and Its Effects on Male Infertility: A Review Study. JRUMS 2018; 17 (3) :253-274. [In Persian]
  55. Duarte J, Magalhaes J, Monteiro L, Almeida-Dias A, Soares J, Appell HJIJoSM. Exercise-induced signs of muscle overuse in children. 1999;20(2):103-8.
  56. Demirbağ R, Yilmaz R, Güzel S, Çelik H, Koçyigit A, Ozcan EJAkdAtAjoc. Effects of treadmill exercise test on oxidative/antioxidative parameters and DNA damage. 2006;6(2):135-40.
  57. Valko M, Rhodes C, Moncol J, Izakovic M, Mazur M. Free radicals, metals and antioxidants in oxidative stress-induced cancer. Chemico-biological interactions. 2006;160(1):1-40.
  58. Shahidi F, Shakeri C, Delfani Z. The effect of eight weeks interval aerobic exercise and consumption of green tea supplementation on oxidative stress indices of inactive young girls. Razi Journal of Medical Sciences. 2019;25(11):72-84. [In Persian]
  59. Liang S, Juan JJAJoMR. Effect of jujube extract on oxidative injury in heart muscles of exhausted training rats. 2011;5(14):1896-9.
  60. Du Fangling LCJJoJIoPE. Effect of Jujube polysaccharide on some biochemical indicators of mouse's blood. 2008:06.
  61. Afzalpour ME, Abtahi Eivari H, Rezazadeh A, Soluki AJTHoMS. Effect of Ziziphus jujuba supplementation before One Session of Acute Resistance Exercise on the Serum Glutathione Peroxidase and Superoxide Dismutase Activity. 2015;21(2):97-104. [In Persian]
  62. Taati M, Alirezaei M, Meshkatalsadat M, Rasoulian B, Kheradmand A, Neamati S. Antioxidant effects of aqueous fruit extract of Ziziphus jujuba on ethanol-induced oxidative stress in the rat testes. Iranian Journal of Veterinary Research. 2011;12(34):39-45. [In Persian]
  63. Afzalpour M, Rezezadeh A, Abtahi H. Effects of Ziziphus Jujube on Total Antioxidant Capacity and Lipid Peroxidation in Young Women after an Intensive Resistance Exercise Session Journal of Sport in Biomotor Sciences. 2014;11(1):16-26. [In Persian]
Journal of Applied Health Studies in Sport Physiology
Volume 10, Issue 1
April 2023
Pages 67-82

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History

  • Receive Date: 14 September 2022
  • Revise Date: 28 December 2022
  • Accept Date: 28 December 2022

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