Effect of 12-weeks high-intensity interval training on SIRT1, PGC-1α and ERRα protein expression in aged rats

Document Type : Research Paper I Open Access I Released under (CC BY-NC 4.0) license

Authors

1 PhD Student, Department of Exercise Physiology, Faculty of Physical Education and Exercise Sciences, University of Tehran, Iran.

2 Professor, Department of Exercise Physiology, Faculty of Physical Education and Exercise Sciences, University of Tehran, Iran.

3 Associate Professor, Department of Exercise Physiology, Faculty of Physical Education and Exercise Sciences, University of Tehran, Iran

4 Associate Professor, Shaheed Beheshti University of Medical Sciences Cellular and Molecular Biology Research center, Tehran, Iran

Abstract

Exercise training improved impaired mitochondrial protein and enzymatic functional induced aging. The purpose of this study was to investigate the effects of 12-weeks of high-intensity interval training on mitochondrial proteins of SIRT1, PGC-1α and ERRα in gastrocnemius muscle in aged rats. In this study 30 aged Wistar rats were randomly divided into HIIT and control group. Exercise training was performed 3 days per week for 8-weeks. Exercise training in the HIIT group was started in the first week by 16 minutes (four intervals (T=2min) at 85-90% VO2max with 4 recovery periods (T=2min) at 45-50% VO2max), which reached 28 minutes in the 12th week. Gastrocnemius was removed 48 h after the final training session. The expression levels of SIRT1, PGC-1α and ERRα proteins in gastrocnemius were assessed by Western blot method. Data analysis was done with independent samples t test. There were no significant differences in body weight and gastrocnemius muscle weight after the intervention. The protein expression of PGC-1α and ERRα were significantly increased in the HIIT compared to control group (p < 0.001). Moreover, SIRT1 expression was significantly elevated in the HIIT compared to control group (P <0.01). It seems that HIIT due to its high intensity and interval nature leads to an increased expression of SIRT1, PGC-1α and ERRα proteins in the gastrocnemius muscle of aging rat models.

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Journal of Applied Health Studies in Sport Physiology
Volume 5, Issue 2
June 2018
Pages 95-102

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History

  • Receive Date: 14 February 2019
  • Revise Date: 28 April 2019
  • Accept Date: 17 May 2019

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