Azarbaijan Shahid Madani UniversityJournal of Applied Health Studies in Sport Physiology2676-650712120250321The effect of eight weeks of high-intensity interval training (HIIT) and vitamin D supplementation on Serum levels of IGF-1, Myostatin and hypertrophy in older menThe effect of eight weeks of high-intensity interval training (HIIT) and vitamin D supplementation on Serum levels of IGF-1, Myostatin and hypertrophy in older men1121449110.22049/jahssp.2022.27754.1456FAJavadVakiliAssociate Professor of Department of Exercise Physiology, Faculty of Sport Sciences, University of Tabriz, Tabriz, IranRaminAmirsasanProfessor of Department of Exercise Physiology, Faculty of Sport Sciences, University of Tabriz, Tabriz, IranKavehBaturakPhD student of Faculty of Physical Education and Sport Sciences, University of Tabriz, Tabriz, IranJournal Article20220517<strong>Aim</strong>: Aging is often associated with progressive loss of muscle mass and strength. high-intensity interval training (HIIT) and vitamin D intake may increase muscle mass, but its mechanism is largely unclear. The aim of the present study was to evaluate effect of eight weeks HIIT and vitamin D supplementation on IGF-1, myostatin and muscular hypertrophy in elderly men<strong>. Methods</strong>: In a experimental research design, 30 inactive elderly men were selected based on fat percentage and aerobic fitness and were randomly divided into three groups: HIIT, HIIT + vitamin D and control. The HIIT training program was performed for eight weeks, three days a week. The HIIT training program was three days a week with an intensity of more 75% reserve heart rate or equivalent to the Borg pressure index of 17-15. Blood samples were taken from the subjects 48 hours before and after the training and supplementation protocol. One-way analysis of variance were used to compare between-group research variables (P <0.05).<strong>Results</strong>: The amount of IGF-1 in both exercise groups (P= 0.003) and exercise + vitamin D (P= 0.0001) was significantly increased compared to the control group, but this difference was not significant between the experimental groups (P= 0.6). Also, the amount of myostatin in both exercise (P= 0.006) and exercise + vitamin D (P= 0.0001) groups was significantly lower than the control group, but this difference was not significant between the experimental groups (P= 0.6). Muscle cross-section showed a significant increase in the two groups (P <0.05) but there was no significant difference between the two groups (P= 0.721). <strong>Conclusions</strong>: It seams HIIT with and without vitamin D increases IGF-1 and muscle cross-section and decreases myostatin. It may reduce muscle atrophy in older men and be effective in older men.<strong>Aim</strong>: Aging is often associated with progressive loss of muscle mass and strength. high-intensity interval training (HIIT) and vitamin D intake may increase muscle mass, but its mechanism is largely unclear. The aim of the present study was to evaluate effect of eight weeks HIIT and vitamin D supplementation on IGF-1, myostatin and muscular hypertrophy in elderly men<strong>. Methods</strong>: In a experimental research design, 30 inactive elderly men were selected based on fat percentage and aerobic fitness and were randomly divided into three groups: HIIT, HIIT + vitamin D and control. The HIIT training program was performed for eight weeks, three days a week. The HIIT training program was three days a week with an intensity of more 75% reserve heart rate or equivalent to the Borg pressure index of 17-15. Blood samples were taken from the subjects 48 hours before and after the training and supplementation protocol. One-way analysis of variance were used to compare between-group research variables (P <0.05).<strong>Results</strong>: The amount of IGF-1 in both exercise groups (P= 0.003) and exercise + vitamin D (P= 0.0001) was significantly increased compared to the control group, but this difference was not significant between the experimental groups (P= 0.6). Also, the amount of myostatin in both exercise (P= 0.006) and exercise + vitamin D (P= 0.0001) groups was significantly lower than the control group, but this difference was not significant between the experimental groups (P= 0.6). Muscle cross-section showed a significant increase in the two groups (P <0.05) but there was no significant difference between the two groups (P= 0.721). <strong>Conclusions</strong>: It seams HIIT with and without vitamin D increases IGF-1 and muscle cross-section and decreases myostatin. It may reduce muscle atrophy in older men and be effective in older men.https://jahssp.azaruniv.ac.ir/article_14491_30997a932519ae62ee7bdd237ff479d2.pdfAzarbaijan Shahid Madani UniversityJournal of Applied Health Studies in Sport Physiology2676-650712120250321The effects of 12 weeks of aerobic exercise and ketogenic diet on secreted frizzled-related protein 5 (SFRP5) levels and metabolic factors in middle-aged men with metabolic syndrome: A double blind placebo-controlled parallel group studyThe effects of 12 weeks of aerobic exercise and ketogenic diet on secreted frizzled-related protein 5 (SFRP5) levels and metabolic factors in middle-aged men with metabolic syndrome: A double blind placebo-controlled parallel group study13271452610.22049/jahssp.2023.28260.1539FABahloulGhorbanianAssociate Professor of Azarbaijan Shahid Madani University, Tabriz, IranAlexeiWongDepartment of Health and Human Performance, Marymount University, Arlington, VA, United StatesAsgarIranpourDepartment of Exercise Physiology, Faculty of Educational Sciences and Psychology, Mohaghegh Ardabili University, Ardabil, IranJournal Article20230123<strong>Aim</strong>: The aim of this study was to investigate the effect of 12 weeks of aerobic exercise combined with a ketogenic diet on Secreted frizzled-related protein 5 (SFRP5) levels and metabolic syndrome factors in middle-aged men with metabolic syndrome. <strong>Methods</strong>: 40 middle-aged men with metabolic syndrome were randomly divided into control (n = 10), aerobic exercise (n = 10), ketogenic diet (n = 10), and aerobic exercise and ketogenic diet (n = 10) groups. The intervention groups performed aerobic exercise and ketogenic diet control for 12 weeks. In the pre-test stages and after 12 weeks, SFRP5 protein levels and metabolic syndrome factors (fasting glucose, lipid profile, blood pressure and waist circumference) were evaluated in four study groups. <strong>Results</strong>: In between-group comparison, the group of aerobic exercise combined with ketogenic diet caused a significant increase in SFRP5 (p=0.04) in middle-aged men with metabolic syndrome compared to the control group. The comparison of other groups in this index did not show any significant difference from each other (p≤0.05). In within-group comparison, the results showed that in all three intervention groups, the level of SFRP5 (aerobic exercise group: p=0.001; ketogenic diet group: p=0.001; and aerobic exercise group with ketogenic diet p=0.001) had a significant increase and the highest increase was in the aerobic exercise group with ketogenic diet.<strong> </strong><strong>Conclusions</strong>: Aerobic exercise combined with a ketogenic diet has better effects than a single aerobic exercise and ketogenic diet to control metabolic risks in middle-aged men with metabolic syndrome.<br /> <strong>Aim</strong>: The aim of this study was to investigate the effect of 12 weeks of aerobic exercise combined with a ketogenic diet on Secreted frizzled-related protein 5 (SFRP5) levels and metabolic syndrome factors in middle-aged men with metabolic syndrome. <strong>Methods</strong>: 40 middle-aged men with metabolic syndrome were randomly divided into control (n = 10), aerobic exercise (n = 10), ketogenic diet (n = 10), and aerobic exercise and ketogenic diet (n = 10) groups. The intervention groups performed aerobic exercise and ketogenic diet control for 12 weeks. In the pre-test stages and after 12 weeks, SFRP5 protein levels and metabolic syndrome factors (fasting glucose, lipid profile, blood pressure and waist circumference) were evaluated in four study groups. <strong>Results</strong>: In between-group comparison, the group of aerobic exercise combined with ketogenic diet caused a significant increase in SFRP5 (p=0.04) in middle-aged men with metabolic syndrome compared to the control group. The comparison of other groups in this index did not show any significant difference from each other (p≤0.05). In within-group comparison, the results showed that in all three intervention groups, the level of SFRP5 (aerobic exercise group: p=0.001; ketogenic diet group: p=0.001; and aerobic exercise group with ketogenic diet p=0.001) had a significant increase and the highest increase was in the aerobic exercise group with ketogenic diet.<strong> </strong><strong>Conclusions</strong>: Aerobic exercise combined with a ketogenic diet has better effects than a single aerobic exercise and ketogenic diet to control metabolic risks in middle-aged men with metabolic syndrome.<br /> https://jahssp.azaruniv.ac.ir/article_14526_27cb45a5fd862e1991105ff40de6b88c.pdfAzarbaijan Shahid Madani UniversityJournal of Applied Health Studies in Sport Physiology2676-650712120250321The effects of short-term taurine supplementation on response of muscle damage and oxidative stress some indicators following a simulated volleyball test in female volleyball playersThe effects of short-term taurine supplementation on response of muscle damage and oxidative stress some indicators following a simulated volleyball test in female volleyball players28391483210.22049/jahssp.2024.29452.1630FAJavadVakiliAssociate Professor Exercise Physiology, Department Exercise Physiology, University of Tabriz,SaeidNikookheslatfaculty of sports science-university of tabriz-tabriz/IranTargolFarzanehExercise physiology,Faculty of sport science,University of Tabriz,Tabriz/Iran.Journal Article20240220Taurine plays a major role in cell physiology, including membrane stabilization, osmoregulation and cytoprotective effects, antioxidant and anti-inflammatory properties. Therefore, the purpose of this study was conducted to identify the effect of taurine supplementation on response of muscle damage and oxidative stress indices following a simulated volleyball test in girl volleyball players. Methods: Twenty-four girls volleyball athletes (with mean age of 18.1±1.6 years, height: 177±9.2 cm and weight: 65.2±9.8 kg) in a randomized, quasi-experimental, double-blind design, were divided in two homogeneous supplement and placebo groups received 3 capsules containing 1g taurine or dextrose. After 14-days the supplementation, all subjects were participated in one session a simulated volleyball test. Blood samples were collected at four phases; baseline, after supplementation period, immediately and 24-hour after the test, were determined for changes in serum of muscle damage (CK and LDH) and oxidative stress (MDA and TAC) indices. Data were analyzed by repeated measure ANOVA and bonferroni post-hoc test at less than 0.05 α≤0.05. Results: The results show that the serum level of CK decreased significantly in the supplement group compared to the placebo group (P=0.012). On the other hand, one session a simulated training caused a significant decrease in TAC (P=0.02) and a significant increase in MDA (P=0.013). While, no significant difference was observed in any of the oxidative stress (TAC and MDA) and muscle damage (LDH) indices between the groups immediately after performing the activity (P≥0.05). Conclusions: Based on the present findings short-term supplementation of taurine may have an effect in reducing muscle damage. However, taurine consumption do not have the ability increased basal antioxidant capacity and also can not decrease the undesirable alterations of serum oxidative stress induced one-session of simulated volleyball test in female volleyball players.Taurine plays a major role in cell physiology, including membrane stabilization, osmoregulation and cytoprotective effects, antioxidant and anti-inflammatory properties. Therefore, the purpose of this study was conducted to identify the effect of taurine supplementation on response of muscle damage and oxidative stress indices following a simulated volleyball test in girl volleyball players. Methods: Twenty-four girls volleyball athletes (with mean age of 18.1±1.6 years, height: 177±9.2 cm and weight: 65.2±9.8 kg) in a randomized, quasi-experimental, double-blind design, were divided in two homogeneous supplement and placebo groups received 3 capsules containing 1g taurine or dextrose. After 14-days the supplementation, all subjects were participated in one session a simulated volleyball test. Blood samples were collected at four phases; baseline, after supplementation period, immediately and 24-hour after the test, were determined for changes in serum of muscle damage (CK and LDH) and oxidative stress (MDA and TAC) indices. Data were analyzed by repeated measure ANOVA and bonferroni post-hoc test at less than 0.05 α≤0.05. Results: The results show that the serum level of CK decreased significantly in the supplement group compared to the placebo group (P=0.012). On the other hand, one session a simulated training caused a significant decrease in TAC (P=0.02) and a significant increase in MDA (P=0.013). While, no significant difference was observed in any of the oxidative stress (TAC and MDA) and muscle damage (LDH) indices between the groups immediately after performing the activity (P≥0.05). Conclusions: Based on the present findings short-term supplementation of taurine may have an effect in reducing muscle damage. However, taurine consumption do not have the ability increased basal antioxidant capacity and also can not decrease the undesirable alterations of serum oxidative stress induced one-session of simulated volleyball test in female volleyball players.https://jahssp.azaruniv.ac.ir/article_14832_cdf796d3598dc8117c375fdbfe16f1bc.pdfAzarbaijan Shahid Madani UniversityJournal of Applied Health Studies in Sport Physiology2676-650712120250321The effect of chess learning and omega-3 supplementation on serum levels of NT-3 and TrkC receptor in elderly womenThe effect of chess learning and omega-3 supplementation on serum levels of NT-3 and TrkC receptor in elderly women40511478810.22049/jahssp.2024.29629.1646FAMalihehYazdizadehbojnurdSadeghCheragh-BirjandiDepartment of Physical Education, Isalmic Azad University, Bojnourd branch, Bojnourd, IranSaraAsghariPhD Candidate in Sports Physiology - Islamic Azad University, Bojnord BranchJournal Article20240404<strong>Aim</strong>: Old age is a period that is accompanied by behavioral, cognitive and movement disorders in addition to physical problems. Neurotrophin-3 (NT-3) is a neurotrophic factor that mainly binds to the receptor tropomyosin kinase C (trkC). NT-3 has been shown to have neuroprotective effects in focal cerebral ischemia. The present study examines the effect of learning chess and consuming omega-3 supplements on the serum levels of neurotrophin-3 and tropomyosin kinase C receptor in elderly women.<strong>Methods</strong>: In this study, 20 elderly women of Bojnord city between the ages of 50 and 70 were randomly divided into four control groups, chess, omega-3 supplement and chess + omega-3 supplement. Subjects in the chess group learned chess for 8 weeks and 3 days a week. The daily intake of omega-3 supplements was two thousand milligrams. Blood sampling was done on two occasions, pre-test and post-test, in the amount of 5 ml from the subjects' brachial vein while sitting. Neurotrophin-3 and tropomyosin kinase C receptor values were measured by ELISA kit for human samples. The data were analyzed using one-way analysis of variance and Tukey's post hoc test at a significance level of less than 0.05.<strong>Results</strong>: The results of the present study showed a significant increase in the serum levels of neurotrophin-3 and tropomyosin kinase C receptor in chess, omega-3 supplement and chess+omega-3 supplement groups compared to the control group (p≤0.05). <strong>Conclusions</strong>: According to the results of the present study, by learning chess and consuming omega-3 supplements, it is possible to improve processes such as learning, memory, and cognitive performance, as well as having a positive effect on neural growth factors and physical condition in old age.<strong>Aim</strong>: Old age is a period that is accompanied by behavioral, cognitive and movement disorders in addition to physical problems. Neurotrophin-3 (NT-3) is a neurotrophic factor that mainly binds to the receptor tropomyosin kinase C (trkC). NT-3 has been shown to have neuroprotective effects in focal cerebral ischemia. The present study examines the effect of learning chess and consuming omega-3 supplements on the serum levels of neurotrophin-3 and tropomyosin kinase C receptor in elderly women.<strong>Methods</strong>: In this study, 20 elderly women of Bojnord city between the ages of 50 and 70 were randomly divided into four control groups, chess, omega-3 supplement and chess + omega-3 supplement. Subjects in the chess group learned chess for 8 weeks and 3 days a week. The daily intake of omega-3 supplements was two thousand milligrams. Blood sampling was done on two occasions, pre-test and post-test, in the amount of 5 ml from the subjects' brachial vein while sitting. Neurotrophin-3 and tropomyosin kinase C receptor values were measured by ELISA kit for human samples. The data were analyzed using one-way analysis of variance and Tukey's post hoc test at a significance level of less than 0.05.<strong>Results</strong>: The results of the present study showed a significant increase in the serum levels of neurotrophin-3 and tropomyosin kinase C receptor in chess, omega-3 supplement and chess+omega-3 supplement groups compared to the control group (p≤0.05). <strong>Conclusions</strong>: According to the results of the present study, by learning chess and consuming omega-3 supplements, it is possible to improve processes such as learning, memory, and cognitive performance, as well as having a positive effect on neural growth factors and physical condition in old age.https://jahssp.azaruniv.ac.ir/article_14788_e6aea563887299a1f39a4c3b0d933cf5.pdfAzarbaijan Shahid Madani UniversityJournal of Applied Health Studies in Sport Physiology2676-650712120250321Comparison of the efficacy of 12 Weeks of Resistance and Combined Training on Activation of AMPK Signaling and Gene Expression of Muscle Atrophy markers in Middle-Aged WomenComparison of the efficacy of 12 Weeks of Resistance and Combined Training on Activation of AMPK Signaling and Gene Expression of Muscle Atrophy markers in Middle-Aged Women52651479010.22049/jahssp.2024.29302.1619FAHiwaMoradianiFaculty of Sport Sciences, Urmia UniversityMohammad RezaZolfaghariAssociate Professor of exercise physiology, Faculty of Sport Sciences, Urmia University, Urmia, Iran.KazemKhodaeiAssistance professor of Exercise Physiology, Faculty of Sport Sciences, Urmia University, Urmia, IranJournal Article20240114<strong>Aim</strong>: Activation of AMPK signaling following exercise is widely associated with the muscle atrophy pathway. The aim of this study was to investigate the comparison of the efficacy of 12 weeks resistance training and combined on the activation of AMPK signaling, muscle glycogen and ATP levels, and gene expression of muscle atrophy markers in middle-aged women. <strong>Methods</strong>: For this purpose, 30 untrained middle-aged women with 40-60 years were randomly divided into 3 groups of incremental resistance training, combined resistance and high intensity interval training, and control. Resistance training protocol included 6 exercises with 3 sets of 5-7 repetitions with 90 seconds rest, and the intensity was 50-80%1RM. high intensity interval training protocol was performed in 5 one-minute interval with an intensity of 60-90% of the maximum heart rate with 2-3 minutes of active rest between each interval, and resistance training included 4 movements in 3 sets with a 90-second rest interval and the number was 5-7 repetitions and the intensity was 50-80% of 1RM. A tissue sample was taken from the vastus lateralis muscle. The difference between the pre-test and post-test data between the groups was evaluated using one-way analysis of variance and Tukey's post hoc test at a significance level of P≤0.05. <strong>Results</strong>: The results showed that muscle glycogen concentration and ATP levels had insignificant changes after 12 weeks of exercise training (P=0.07). Also, the results showed that muscle AMPK levels decreased significantly after 12 weeks of resistance and combined training (P=0.005). The gene expression of Atrogin-1/MAFbx, MuRF-1 and FOXO3a in muscle decreased significantly after 12 weeks of resistance training (P=0.01). <strong>Conclusions</strong>: Although there was no difference between the combined and resistance training groups in all variables. However, the effect of resistance training in reducing of AMPK and muscle atrophy genes expression was stronger.<strong>Aim</strong>: Activation of AMPK signaling following exercise is widely associated with the muscle atrophy pathway. The aim of this study was to investigate the comparison of the efficacy of 12 weeks resistance training and combined on the activation of AMPK signaling, muscle glycogen and ATP levels, and gene expression of muscle atrophy markers in middle-aged women. <strong>Methods</strong>: For this purpose, 30 untrained middle-aged women with 40-60 years were randomly divided into 3 groups of incremental resistance training, combined resistance and high intensity interval training, and control. Resistance training protocol included 6 exercises with 3 sets of 5-7 repetitions with 90 seconds rest, and the intensity was 50-80%1RM. high intensity interval training protocol was performed in 5 one-minute interval with an intensity of 60-90% of the maximum heart rate with 2-3 minutes of active rest between each interval, and resistance training included 4 movements in 3 sets with a 90-second rest interval and the number was 5-7 repetitions and the intensity was 50-80% of 1RM. A tissue sample was taken from the vastus lateralis muscle. The difference between the pre-test and post-test data between the groups was evaluated using one-way analysis of variance and Tukey's post hoc test at a significance level of P≤0.05. <strong>Results</strong>: The results showed that muscle glycogen concentration and ATP levels had insignificant changes after 12 weeks of exercise training (P=0.07). Also, the results showed that muscle AMPK levels decreased significantly after 12 weeks of resistance and combined training (P=0.005). The gene expression of Atrogin-1/MAFbx, MuRF-1 and FOXO3a in muscle decreased significantly after 12 weeks of resistance training (P=0.01). <strong>Conclusions</strong>: Although there was no difference between the combined and resistance training groups in all variables. However, the effect of resistance training in reducing of AMPK and muscle atrophy genes expression was stronger.https://jahssp.azaruniv.ac.ir/article_14790_8ae36db695caa655a0ee78847fdfccf2.pdfAzarbaijan Shahid Madani UniversityJournal of Applied Health Studies in Sport Physiology2676-650712120250321The effect of eight weeks of endurance training and alkaline consumption on inflammatory biomarkers and oxidative stress in obese men with metabolic syndromeThe effect of eight weeks of endurance training and alkaline consumption on inflammatory biomarkers and oxidative stress in obese men with metabolic syndrome66801479710.22049/jahssp.2024.29521.1638FAحسنNaghizadehAssistant Professor of exercise physiology, Department of Sport Sciences, Ardakan University, P.O. Box 184, Ardakan, Irann.Journal Article20240310<strong>Aim</strong>: Nowadays, the intervention of physical exercises along with sports nutrition is considered as an effective treatment method in modulating inflammatory and oxidative responses in obese people with metabolic syndrome. Therefore, the aim of this research is to investigate the effect of eight weeks of endurance training and alkaline consumption on interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-α), nitric oxide (NO) and total thiol (T-SH) in Obese men with metabolic syndrome.<strong> Methods</strong>: The current research was a semi-experimental one-blind type. For this purpose, 56 men (average age= 33.27±3.11 years, average weight= 94.16±2.91 kg and BMI= 32.56±1.32 kg/m<sup>2</sup>) voluntarily participated in this research. Subjects were randomly divided into four groups: endurance training, endurance training with alkaline water, alkaline water and control. The training intervention was implemented three sessions a week for eight weeks. Alkaline water (pH 9.5) was received at the rate of 10 kg/mL after each training session. Blood samples were taken before training and 48 hours after the last training session to measure inflammatory biomarkers and oxidative stress. <strong>Results</strong>: The results showed that the values of IL-6 and TNF-α in the group of endurance training with alkaline water and endurance training compared to the groups of alkaline water and control had a significant decrease, and the values of NO and T-SH had a significant increase (p<0.05). The highest percentage of significant changes in the values of IL-6, TNF-α, NO and T-SH were assigned to the training group with alkaline water. Alkaline water alone did not cause significant changes (P>0.05). <strong>Conclusions</strong>: Therefore, the combined effect of endurance exercise and alkaline water improves inflammatory responses and oxidative stress in obese men with metabolic syndrome.<strong>Aim</strong>: Nowadays, the intervention of physical exercises along with sports nutrition is considered as an effective treatment method in modulating inflammatory and oxidative responses in obese people with metabolic syndrome. Therefore, the aim of this research is to investigate the effect of eight weeks of endurance training and alkaline consumption on interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-α), nitric oxide (NO) and total thiol (T-SH) in Obese men with metabolic syndrome.<strong> Methods</strong>: The current research was a semi-experimental one-blind type. For this purpose, 56 men (average age= 33.27±3.11 years, average weight= 94.16±2.91 kg and BMI= 32.56±1.32 kg/m<sup>2</sup>) voluntarily participated in this research. Subjects were randomly divided into four groups: endurance training, endurance training with alkaline water, alkaline water and control. The training intervention was implemented three sessions a week for eight weeks. Alkaline water (pH 9.5) was received at the rate of 10 kg/mL after each training session. Blood samples were taken before training and 48 hours after the last training session to measure inflammatory biomarkers and oxidative stress. <strong>Results</strong>: The results showed that the values of IL-6 and TNF-α in the group of endurance training with alkaline water and endurance training compared to the groups of alkaline water and control had a significant decrease, and the values of NO and T-SH had a significant increase (p<0.05). The highest percentage of significant changes in the values of IL-6, TNF-α, NO and T-SH were assigned to the training group with alkaline water. Alkaline water alone did not cause significant changes (P>0.05). <strong>Conclusions</strong>: Therefore, the combined effect of endurance exercise and alkaline water improves inflammatory responses and oxidative stress in obese men with metabolic syndrome.https://jahssp.azaruniv.ac.ir/article_14797_46281247891a238839c956ddd9e6cd62.pdfAzarbaijan Shahid Madani UniversityJournal of Applied Health Studies in Sport Physiology2676-650712120250321Effect of High_Intensity Interval Training and Acetyl_L_Carnitine supplementation on Thioredoxin and alpha-synuclein in the brain tissue of male rat model of parkinson,s diseaseEffect of High_Intensity Interval Training and Acetyl_L_Carnitine supplementation on Thioredoxin and alpha-synuclein in the brain tissue of male rat model of parkinson,s disease81941480010.22049/jahssp.2024.29499.1636FAYousefGhaffariExercise Physiology Ph.D. student, Physical Education & Sport Sciences Department, Faculty of Humanities, Tabriz Branch, Islamic Azad University, Tabriz, IranFarzadZehsazAssociate Professor, Department of Physical Education &amp; Sport Sciences, Faculty of Humanities, Tabriz Branch, Islamic Azad University, TabrizMir AlirezaNourazarAssistant Professor, Department of Basic Sciences, Faculty of Veterinary Medicine, Tabriz Medical Sciences, Islamic Azad University, Tabriz, Iran.HasnPorraziAssistant Professor, Department of Physical Education and Sports Science, Faculty of Social Sciences, Imam Khomeini International University, Qazvin, Iran.DaryoushMohajeriProfessor, Department of Pathobiology, Faculty of Veterinary Medicine, Tabriz Medical Sciences, Islamic Azad University, Tabriz, Iran.Journal Article20240303<strong>Aim</strong>: Parkinson's disease is a neurodegenerative disease characterized by the destruction of dopaminergic neurons in the substantia nigra region of the brain. Alpha-synuclein is a protein that forms plaques called Lewy bodies in Parkinson's disease and is considered the main cause of brain tissue damage. The purpose of the present study was to determine the effect of an intense interval training period and acetyl-L-carnitine (ALC) supplementation on alpha-synuclein and thioredoxin (TRX) protein levels in the brains of 6-hydroxydopamine-induced parkinsonian rats. Research method: The statistical population of this study included 36 Wistar male rats, which were randomly divided into 6 equal groups: healthy control group, sham surgery group, Parkinson's control group, Parkinson's exercise group, Parkinson's supplement group, Parkinson's exercise and supplement group. This experimental research was carried out during 12 weeks of training and the drug receiving groups received ALC supplement once a day for 12 weeks with a dose of 100 mg/kg by gavage. Behavioral tests were performed to confirm the creation of the Parkinsonian model. Tissue samples were obtained 48 hours after the last exercise program. Results: The findings showed that after 12 weeks of HIIT training and ALC consumption, there was a significant difference in TRX (P=0.001) and alpha-synuclein (P=0.001) values between the study groups. The results of the follow-up test showed that the values of TRX and alpha-synuclein in the exercise+supplement group increased (P=0.001) and decreased (P=0.002) respectively, compared to the Parkinson's control group. There was no significant difference in the values of alpha-synuclein in the training (p=0.114) and supplement (p=0.325) groups compared to the Parkinson's control group. Conclusion: Intense intermittent exercise with ALC supplement may be effective in improving cell damage and function of Parkinson's patients by modulating some oxidative regulatory pathways, including increasing TRX antioxidant enzyme and reducing brain tissue alpha-synuclein accumulation.
<strong>Aim</strong>: Parkinson's disease is a neurodegenerative disease characterized by the destruction of dopaminergic neurons in the substantia nigra region of the brain. Alpha-synuclein is a protein that forms plaques called Lewy bodies in Parkinson's disease and is considered the main cause of brain tissue damage. The purpose of the present study was to determine the effect of an intense interval training period and acetyl-L-carnitine (ALC) supplementation on alpha-synuclein and thioredoxin (TRX) protein levels in the brains of 6-hydroxydopamine-induced parkinsonian rats. Research method: The statistical population of this study included 36 Wistar male rats, which were randomly divided into 6 equal groups: healthy control group, sham surgery group, Parkinson's control group, Parkinson's exercise group, Parkinson's supplement group, Parkinson's exercise and supplement group. This experimental research was carried out during 12 weeks of training and the drug receiving groups received ALC supplement once a day for 12 weeks with a dose of 100 mg/kg by gavage. Behavioral tests were performed to confirm the creation of the Parkinsonian model. Tissue samples were obtained 48 hours after the last exercise program. Results: The findings showed that after 12 weeks of HIIT training and ALC consumption, there was a significant difference in TRX (P=0.001) and alpha-synuclein (P=0.001) values between the study groups. The results of the follow-up test showed that the values of TRX and alpha-synuclein in the exercise+supplement group increased (P=0.001) and decreased (P=0.002) respectively, compared to the Parkinson's control group. There was no significant difference in the values of alpha-synuclein in the training (p=0.114) and supplement (p=0.325) groups compared to the Parkinson's control group. Conclusion: Intense intermittent exercise with ALC supplement may be effective in improving cell damage and function of Parkinson's patients by modulating some oxidative regulatory pathways, including increasing TRX antioxidant enzyme and reducing brain tissue alpha-synuclein accumulation.
https://jahssp.azaruniv.ac.ir/article_14800_72c805d72a5617e9da6cc3522b55cbfe.pdfAzarbaijan Shahid Madani UniversityJournal of Applied Health Studies in Sport Physiology2676-650712120250321Cardiovascular responses (HRV, BP, and RPP) following a resistance exercise session with a training mask and blood flow restriction in In young male athletesCardiovascular responses (HRV, BP, and RPP) following a resistance exercise session with a training mask and blood flow restriction in In young male athletes951081480510.22049/jahssp.2024.29728.1653FAAbolfazlRahmaniMSc of Exercise Physiology, University of GuilanJavadMehrabaniDep. Exercise Physiology, University of GuilanJournal Article20240512<strong>Aim</strong>: Resistance training with innovative training methods elicits various cardiac and vascular responses. Therefore, the main objective of this research was to investigate the rate pressure product, blood pressure response, and Cardiac Autonomic Function to resistance activity with a training mask and blood flow restriction in young male athletes. <strong>Methods</strong>: Eleven trained men (average age 29.2 ± 5.7 years and body mass index 25.3 ± 2.7 kg/m²) with a history of resistance training in the past year participated in a three-week randomized, crossover study using three different methods: traditional (control) resistance training (1RM 75%-80%), resistance training with blood flow restriction, and hypoxic masks (1RM 20%-50%). The resistance training consisted of one set of 20 repetitions and three sets of 15 repetitions of leg extension movements. Cardiovascular responses were recorded before, immediately after, and every 5 minutes for 60 minutes. The repeated measures analysis of variance method using SPSS software (version 23) was applied to examine the significance level of p<0.05. <strong>Results: </strong>Based on the results obtained, each of the three training methods can lead to a significant increase in hemodynamic responses, including heart rate variability and blood pressure, except for diastolic blood pressure, following the activity. This increase was observed immediately after the exercise, but no significant difference was observed between the groups. (p< 0.05). <strong>Conclusions</strong>: The findings of this study indicate that a single bout of resistance exercise, with or without blood flow restriction and a hypoxia mask, does not result in distinct cardiovascular responses compared to each other. This study also significantly confirms the effectiveness of such exercise methods, showing that low-intensity exercises with restrictions can achieve goals expected from high-intensity exercises.
<strong>Aim</strong>: Resistance training with innovative training methods elicits various cardiac and vascular responses. Therefore, the main objective of this research was to investigate the rate pressure product, blood pressure response, and Cardiac Autonomic Function to resistance activity with a training mask and blood flow restriction in young male athletes. <strong>Methods</strong>: Eleven trained men (average age 29.2 ± 5.7 years and body mass index 25.3 ± 2.7 kg/m²) with a history of resistance training in the past year participated in a three-week randomized, crossover study using three different methods: traditional (control) resistance training (1RM 75%-80%), resistance training with blood flow restriction, and hypoxic masks (1RM 20%-50%). The resistance training consisted of one set of 20 repetitions and three sets of 15 repetitions of leg extension movements. Cardiovascular responses were recorded before, immediately after, and every 5 minutes for 60 minutes. The repeated measures analysis of variance method using SPSS software (version 23) was applied to examine the significance level of p<0.05. <strong>Results: </strong>Based on the results obtained, each of the three training methods can lead to a significant increase in hemodynamic responses, including heart rate variability and blood pressure, except for diastolic blood pressure, following the activity. This increase was observed immediately after the exercise, but no significant difference was observed between the groups. (p< 0.05). <strong>Conclusions</strong>: The findings of this study indicate that a single bout of resistance exercise, with or without blood flow restriction and a hypoxia mask, does not result in distinct cardiovascular responses compared to each other. This study also significantly confirms the effectiveness of such exercise methods, showing that low-intensity exercises with restrictions can achieve goals expected from high-intensity exercises.
https://jahssp.azaruniv.ac.ir/article_14805_633bb7da65a448dbd9875b2a9f4f068d.pdfAzarbaijan Shahid Madani UniversityJournal of Applied Health Studies in Sport Physiology2676-650712120250321The effect of aerobic and resistance training during ketogenic diet on fetuin A serum levels, blood lipid profile and liver enzymes in overweight and obese boysThe effect of aerobic and resistance training during ketogenic diet on fetuin A serum levels, blood lipid profile and liver enzymes in overweight and obese boys1081201481810.22049/jahssp.2024.29601.1643FASajjadMobaseriPhd student of Sports Physiology Department, Faculty of Sports Sciences, Urmia University, Urmia- Iran.KazemKhodaeiAssistant Professor, Department of Sports Physiology, Faculty of Sports Sciences, Urmia University, Urmia- Iran.MohammadrezaZolfagari DidaniAssociate Professor, Department of Sports Physiology, Faculty of Sports Sciences, Urmia University, Urmia- Iran.Journal Article20240326<strong>Aim</strong>: Liver haptokines are influential factors in diseases related to obesity. Exercise and nutrition are two basic interventions in controlling obesity. The aim of this study was the effect of aerobic and resistance training during ketogenic diet on serum fetoin A levels, blood lipid profile and liver enzymes in overweight and obese boys. <strong>Methodology</strong>: 36 overweight and obese inactive male students who had a body mass index above 25 kg/m2 were selected voluntarily and available sample. Then they were randomly divided into three groups: aerobic training during ketogenic diet (12 people), resistance training during ketogenic diet (12 people) and ketogenic diet alone (12 people). Aerobic exercises consisted of moderate intensity running for 6 weeks and three sessions per week. Resistance training consisted of circuit strength training for six weeks and three sessions each week. The ketogenic diet consisted of restricting carbohydrate intake to less than or equal to 10% of total daily energy intake. Fasting blood sample was taken in pre-test and post-test. A two-way mixed analysis of variance test was used for statistical analysis. <strong>Findings:</strong> According to the results of the interaction of time in the group, no significant difference was found between the groups in the amount of fetoin A (p>0.05), but the time factor showed a significant difference (p=0.001) and also every The three groups had a significant decrease in fetoin A compared to the pre-test state. The interaction of time in the group did not show any significant difference between the groups in the lipid profile and liver enzymes (ALT and AST) (p>0.05). However, the results within the group showed a significant decrease in low-density lipoprotein, total cholesterol, ALT and AST, while a significant increase in high-density lipoprotein in all three groups (p<0.05). <strong>Conclusion</strong>: According to the results, it seems that both types of aerobic and resistance exercises during the ketogenic diet can be a useful intervention to improve fetoin A, liver enzymes and lipid profile in overweight and obese people compared to the pre-test. be made But no difference was observed between the two types of exercise. Also, both aerobic and resistance exercises during the ketogenic diet were not superior to the ketogenic diet alone in the measured heptokine factors and lipid profile.<strong>Aim</strong>: Liver haptokines are influential factors in diseases related to obesity. Exercise and nutrition are two basic interventions in controlling obesity. The aim of this study was the effect of aerobic and resistance training during ketogenic diet on serum fetoin A levels, blood lipid profile and liver enzymes in overweight and obese boys. <strong>Methodology</strong>: 36 overweight and obese inactive male students who had a body mass index above 25 kg/m2 were selected voluntarily and available sample. Then they were randomly divided into three groups: aerobic training during ketogenic diet (12 people), resistance training during ketogenic diet (12 people) and ketogenic diet alone (12 people). Aerobic exercises consisted of moderate intensity running for 6 weeks and three sessions per week. Resistance training consisted of circuit strength training for six weeks and three sessions each week. The ketogenic diet consisted of restricting carbohydrate intake to less than or equal to 10% of total daily energy intake. Fasting blood sample was taken in pre-test and post-test. A two-way mixed analysis of variance test was used for statistical analysis. <strong>Findings:</strong> According to the results of the interaction of time in the group, no significant difference was found between the groups in the amount of fetoin A (p>0.05), but the time factor showed a significant difference (p=0.001) and also every The three groups had a significant decrease in fetoin A compared to the pre-test state. The interaction of time in the group did not show any significant difference between the groups in the lipid profile and liver enzymes (ALT and AST) (p>0.05). However, the results within the group showed a significant decrease in low-density lipoprotein, total cholesterol, ALT and AST, while a significant increase in high-density lipoprotein in all three groups (p<0.05). <strong>Conclusion</strong>: According to the results, it seems that both types of aerobic and resistance exercises during the ketogenic diet can be a useful intervention to improve fetoin A, liver enzymes and lipid profile in overweight and obese people compared to the pre-test. be made But no difference was observed between the two types of exercise. Also, both aerobic and resistance exercises during the ketogenic diet were not superior to the ketogenic diet alone in the measured heptokine factors and lipid profile.https://jahssp.azaruniv.ac.ir/article_14818_a05377cf338f62d088163de07aecf466.pdfAzarbaijan Shahid Madani UniversityJournal of Applied Health Studies in Sport Physiology2676-650712120250321The effect of two months of intense intermittent exercise with sodium citrate supplementation on intracellular redox status and oxidative stress indices in diabetic ratsThe effect of two months of intense intermittent exercise with sodium citrate supplementation on intracellular redox status and oxidative stress indices in diabetic rats1211351481910.22049/jahssp.2024.29688.1651FAMalahatKashfi MoghadamDepartment of Sport Physiology, Faculty of Educational Sciences and Psychology, University of Mohaghegh Ardabili, Ardabil, IranFarnazSeyffi EskishahrDepartment of Sport Physiology, Faculty of Educational Sciences and Psychology, University of Mohaghegh Ardabili, Ardabil, IranRoghayyehAfroundehDepartment of Exercise Physiology, Faculty of Educational Sciences and Psychology, University of Mohaghegh Ardabili, Ardabil, IranLotfaliBolboliDepartment of Sport Physiology, Faculty of Education and Psychology, University of Mohaghegh Ardabili, Ardabil, IranJournal Article20240422<strong>Aim</strong>: Alteration of cellular redox signal is effective in the incidence of diabetes and the development of its complications, so the aim of this study was to investigate the effect of two months high-intensity interval training with sodium citrate supplementation on intracellular redox status and oxidative stress indices in the heart of diabetic rats.<strong> Methods</strong>: This interventional study was performed on 50 white male rats (3 months old) with weight rang of 225-300 g in five groups: healthy control, diabetic control, diabetic-training, diabetic-sodium citrate supplementation, and diabetic-sodium citrate supplementation-training. Rats in both groups of training and training + supplementation performed interval training with an intensity of 90% of maximum running speed on a treadmill for 8 weeks, 5 days a week. In addition to the exercise, the rats in the exercise+supplement group received 15 mmol/L of sodium citrate supplement in the form of water soluble daily. Oxidative stress indices were measured using the ELISA kit with a coefficient of variation of 15%. Data were analyzed using one-way ANOVA and LSD post hoc test in SPPS26 statistical software (p<0.05).<strong> Results</strong>: There was a significant difference between the healthy and diabetic groups in the levels of GSH, GSSG، GSH/GSSG, SOD (p<0.05). The level of GSH, GSH/GSSG, and SOD were lower in high-intensity interval training compared to diabetic control group (p<0.05) but sodium citrate supplementation significantly increased the level of these variables in diabetic rats. Significant reduction in the level of GSSG was observed in the diabetic-supplement and diabetic-supplement-exercise groups, compared to the diabetic-exercise and control-diabetic groups (p<0.05). The difference in the means showed that this decrease in GSSG level was more in the diabetic-supplement-exercise group compared to the other diabetic groups.<strong> </strong>
<strong>Conclusions</strong> The results of the present study showed that interval training with an intensity of 90% of the maximum running speed can increase oxidative stress indices in the heart of diabetic rats, so it is recommended to do this type of exercise with caution and using sodium citrate supplementation or other antioxidant supplements in diabetic patients.<strong>Aim</strong>: Alteration of cellular redox signal is effective in the incidence of diabetes and the development of its complications, so the aim of this study was to investigate the effect of two months high-intensity interval training with sodium citrate supplementation on intracellular redox status and oxidative stress indices in the heart of diabetic rats.<strong> Methods</strong>: This interventional study was performed on 50 white male rats (3 months old) with weight rang of 225-300 g in five groups: healthy control, diabetic control, diabetic-training, diabetic-sodium citrate supplementation, and diabetic-sodium citrate supplementation-training. Rats in both groups of training and training + supplementation performed interval training with an intensity of 90% of maximum running speed on a treadmill for 8 weeks, 5 days a week. In addition to the exercise, the rats in the exercise+supplement group received 15 mmol/L of sodium citrate supplement in the form of water soluble daily. Oxidative stress indices were measured using the ELISA kit with a coefficient of variation of 15%. Data were analyzed using one-way ANOVA and LSD post hoc test in SPPS26 statistical software (p<0.05).<strong> Results</strong>: There was a significant difference between the healthy and diabetic groups in the levels of GSH, GSSG، GSH/GSSG, SOD (p<0.05). The level of GSH, GSH/GSSG, and SOD were lower in high-intensity interval training compared to diabetic control group (p<0.05) but sodium citrate supplementation significantly increased the level of these variables in diabetic rats. Significant reduction in the level of GSSG was observed in the diabetic-supplement and diabetic-supplement-exercise groups, compared to the diabetic-exercise and control-diabetic groups (p<0.05). The difference in the means showed that this decrease in GSSG level was more in the diabetic-supplement-exercise group compared to the other diabetic groups.<strong> </strong>
<strong>Conclusions</strong> The results of the present study showed that interval training with an intensity of 90% of the maximum running speed can increase oxidative stress indices in the heart of diabetic rats, so it is recommended to do this type of exercise with caution and using sodium citrate supplementation or other antioxidant supplements in diabetic patients.https://jahssp.azaruniv.ac.ir/article_14819_dacf25c5e121cfe5c0e3fccb7f85df39.pdfAzarbaijan Shahid Madani UniversityJournal of Applied Health Studies in Sport Physiology2676-650712120250321The effect of exercise training on circulating levels of BDNF and IGF-1 in children and adolescents: a systematic review with meta-analysisThe effect of exercise training on circulating levels of BDNF and IGF-1 in children and adolescents: a systematic review with meta-analysis1361541483110.22049/jahssp.2024.29758.1658FAMousaKhalafiUniversity of TehranDepartment of Physical Education and Sport Sciences, Faculty of Humanities, University of Kashan, Kashan, Iran0000-0001-6465-1281MaryamAghaeinejadDepartment of Sports Sciences, Faculty of Humanities, University of Kashan, Kashan, Iran0009-0002-7077-2519MaryamSaresangiDepartment of Sports Sciences, Faculty of Humanities, University of Kashan, Kashan, IranJournal Article20240521<strong>Aim</strong>. Brain-derived nerve growth factor (BDNF) and insulin-like growth factor (IGF-1) are known neuroprotective factors that are affected by exercise training. However, the effect of exercise training on these neuroprotective biomarkers in children and adolescents has been reported inconsistently. Therefore, the aim of the meta-analysis is to investigate the effect of exercise training on blood circulating levels of BDNF and IGF-1 in children and adolescents. <strong>Method</strong>. A comprehensive search was conducted in PubMed, Web of Science, Scopus, and Google Scholar databases, as well as Iranian databases from the inception to December 6, 2023. The inclusion criteria for this study based on the PICOS guidelines (population, intervention, comparison, variable and type of study) include: 1) children and adolescents 2) exercise training with an intervention duration of more than 2 weeks, 3) studies with a control group or measurement values in the pre-test; 4) BDNF, IGF-1 measured in blood circulation (serum and plasma) and 5) single group studies (without control group) and two group studies (with control group). To determine the effect size, SMD and 95% confidence intervals (CIs) were calculated using the random model. <strong>Results</strong>. A total of 21 studies including 855 children and adolescent individuals with an average age of 7 to 17 years were included in the meta-analysis. Based on meta-analysis results, exercise training leads to a significant increase in BDNF levels with medium effect size [SMD = 0.51, (Cl: 0.91 to 0.11), P=0.01]. Nevertheless, exercise training did not have a significant effect on IGF-1 levels [SMD = 0.25 (Cl: 0.61 to -0.11), P=0.17]. <strong>Conclusion</strong>. Exercise training may lead to beneficial effects in improving cognitive function and metabolic status of children and adolescents by increasing BDNF. However, exercise training did not have significant effects on IGF-1, and further studies is still needed to determine the role of exercise training.<strong>Aim</strong>. Brain-derived nerve growth factor (BDNF) and insulin-like growth factor (IGF-1) are known neuroprotective factors that are affected by exercise training. However, the effect of exercise training on these neuroprotective biomarkers in children and adolescents has been reported inconsistently. Therefore, the aim of the meta-analysis is to investigate the effect of exercise training on blood circulating levels of BDNF and IGF-1 in children and adolescents. <strong>Method</strong>. A comprehensive search was conducted in PubMed, Web of Science, Scopus, and Google Scholar databases, as well as Iranian databases from the inception to December 6, 2023. The inclusion criteria for this study based on the PICOS guidelines (population, intervention, comparison, variable and type of study) include: 1) children and adolescents 2) exercise training with an intervention duration of more than 2 weeks, 3) studies with a control group or measurement values in the pre-test; 4) BDNF, IGF-1 measured in blood circulation (serum and plasma) and 5) single group studies (without control group) and two group studies (with control group). To determine the effect size, SMD and 95% confidence intervals (CIs) were calculated using the random model. <strong>Results</strong>. A total of 21 studies including 855 children and adolescent individuals with an average age of 7 to 17 years were included in the meta-analysis. Based on meta-analysis results, exercise training leads to a significant increase in BDNF levels with medium effect size [SMD = 0.51, (Cl: 0.91 to 0.11), P=0.01]. Nevertheless, exercise training did not have a significant effect on IGF-1 levels [SMD = 0.25 (Cl: 0.61 to -0.11), P=0.17]. <strong>Conclusion</strong>. Exercise training may lead to beneficial effects in improving cognitive function and metabolic status of children and adolescents by increasing BDNF. However, exercise training did not have significant effects on IGF-1, and further studies is still needed to determine the role of exercise training.https://jahssp.azaruniv.ac.ir/article_14831_5f502416d58200914087650b228d02aa.pdfAzarbaijan Shahid Madani UniversityJournal of Applied Health Studies in Sport Physiology2676-650712120250321Evaluation of the effect of high intensity interval training (HIIT) and melatonin on oxidative stress in rats with renal ischemia-reperfusionEvaluation of the effect of high intensity interval training (HIIT) and melatonin on oxidative stress in rats with renal ischemia-reperfusion1551671484110.22049/jahssp.2024.29760.1659FAFaribaPourasgharPhD Student of Exercise Physiology, Department of Physical Education and Sport Science, Islamic Azad University of Tabriz, Iran.JabbarBashiriTabriz Branch, Islamic Azad UniversityRoghayehPoozesh Jadidi2. Assist Prof, Department of Physical Education, Tabriz branch, Islamic Azad university, Tabriz, IranHasnPorraziAssistant Professor, Department of Physical Education and Sports Science, Faculty of Social Sciences, Imam Khomeini International University, Qazvin, Iran.AlirezaNourazarDepartment of Veterinary Medicine, Tabriz Azad University of Medical Sciences, Iran.Journal Article20240601<strong>Aim</strong>: This research investigated the effect of high intensity interval training (HIIT) and melatonin supplementation on oxidative stress markers, antioxidant enzyme activity, creatinine (Cr) and blood urea nitrogen (BUN) concentrations, as well as histopathological changes to evaluate kidney function in male rats with ischemia renal reperfusion.<strong> Methods</strong>: 30 male Wistar rats were randomly divided into 5 groups, healthy, Ischemia/Reperfusion (IR), IR + intense interval training, IR + melatonin supplement and IR + high intensive interval training + melatonin supplement. Except for the healthy group, the animals were subjected to ischemia-reperfusion. The HIIT protocol was implemented for 12 weeks (5 days per week). Oxidative indicators of SOD, GPX and MDA were measured by spectrophotometric method and for BUN and Cr indicators; urea was quantitatively detected by Urease-GLDH and creatinine by JAFFE method in plasma serum using photometric method. Because of normal distribution of parameters, the data were analysed by one-way analysis of variance and comparison test of two populations at significance level of (p<0.05). <strong>Results:</strong> A decrease in kidney function after IR was observed with an increase in Cr, BUN and MDA levels and a decrease in GPX and SOD (P < 0.05). Melatonin consumption decreased BUN, Cr and MDA and increased SOD, GPX (P < 0.05) compared to the ischemic group. In the intense interval training group, BUN decreased and GPX increased compared to the ischemic group (P<0.05). Performing intense interval training along with melatonin supplementation decreased BUN and Cr levels and increased SOD and GPX levels (P < 0.05). The histopathological results showed that intense interval training along with melatonin consumption had the least damage to the kidney tissue. <strong>Conclusions</strong>: The high-intensity interval training and melatonin supplementation are effective in protecting kidney tissue against apoptotic damage and inflammation caused by renal ischemia. <strong>Aim</strong>: This research investigated the effect of high intensity interval training (HIIT) and melatonin supplementation on oxidative stress markers, antioxidant enzyme activity, creatinine (Cr) and blood urea nitrogen (BUN) concentrations, as well as histopathological changes to evaluate kidney function in male rats with ischemia renal reperfusion.<strong> Methods</strong>: 30 male Wistar rats were randomly divided into 5 groups, healthy, Ischemia/Reperfusion (IR), IR + intense interval training, IR + melatonin supplement and IR + high intensive interval training + melatonin supplement. Except for the healthy group, the animals were subjected to ischemia-reperfusion. The HIIT protocol was implemented for 12 weeks (5 days per week). Oxidative indicators of SOD, GPX and MDA were measured by spectrophotometric method and for BUN and Cr indicators; urea was quantitatively detected by Urease-GLDH and creatinine by JAFFE method in plasma serum using photometric method. Because of normal distribution of parameters, the data were analysed by one-way analysis of variance and comparison test of two populations at significance level of (p<0.05). <strong>Results:</strong> A decrease in kidney function after IR was observed with an increase in Cr, BUN and MDA levels and a decrease in GPX and SOD (P < 0.05). Melatonin consumption decreased BUN, Cr and MDA and increased SOD, GPX (P < 0.05) compared to the ischemic group. In the intense interval training group, BUN decreased and GPX increased compared to the ischemic group (P<0.05). Performing intense interval training along with melatonin supplementation decreased BUN and Cr levels and increased SOD and GPX levels (P < 0.05). The histopathological results showed that intense interval training along with melatonin consumption had the least damage to the kidney tissue. <strong>Conclusions</strong>: The high-intensity interval training and melatonin supplementation are effective in protecting kidney tissue against apoptotic damage and inflammation caused by renal ischemia. https://jahssp.azaruniv.ac.ir/article_14841_d79167af20be6850a54eec2bbac1b5f2.pdf