Azarbaijan Shahid Madani University Journal of Applied Health Studies in Sport Physiology 2676-6507 Articles in Press 2026 02 23 Investigation the effect of eight weeks of endurance training combined with BCAA supplementation on the genes expression of associated with muscle atrophy in the soleus muscle of Parkinson's rats Investigation the effect of eight weeks of endurance training combined with BCAA supplementation on the genes expression of associated with muscle atrophy in the soleus muscle of Parkinson's rats 15118 10.22049/jahssp.2026.31264.1799 FA Lotfali Bolboli Professor, Department of Exercise Physiology, Faculty of Educational Sciences and Psychology, University of Mohaghegh Ardabili, Ardabil, Ira Mojdeh Khajehlandi Postdoctoral, Department of Exercise Physiology, Faculty of Educational Sciences and Psychology, University of Mohaghegh Ardabili, Ardabil, Iran Hojatolah Shahavand PhD, Department of Physical Education and Sport Science, Kermanshah Branch, Islamic Azad University, Kermanshah, Iran Journal Article 2025 11 30 Abstract Aim: Parkinson's disease is a chronic neurological disorder that compromises motor and non-motor functions and leads to a decrease in the quality of life and functional capacity of individuals. Therefore, the aim of the present study was to investigate the effect of eight weeks of moderate-intensity endurance training with BCAA supplementation on the gene expression of MuRF1, Atrogin-1, and Myostatin in the soleus muscle of Parkinsonian-induced rats. Methods: In the present experimental study, 25 male Wistar rats, at 12 weeks of age and 180-200 gr were used. The rats were divided into following groups (n=5): healthy control (HC), Parkinson control (PC), Parkinson + training (PT), Parkinson + BCAA (PB), and Parkinson + training + BCAA (PTB) groups. Eight weeks of moderate-intensity endurance training (5 days a week) performed on a treadmill. One-way analysis of variance with the Tukey's post-hoc test used to analyze the data (with a significance level of P≤0.05). Results: The results showed that after Parkinson's induction, genes expression levels of MuRF1, Atrogin-1, and Myostatin in the soleus muscle of rats significantly increased (P=0.001). There was a significant decrease in genes expression of MuRF1, Atrogin-1, and Myostatin (P=0.001) only in the moderate-intensity exercise training with BCAA supplementation group after eight weeks of interventions. Conclusion: Although moderate-intensity endurance training and BCAA supplementation alone did not affect gene expression, combining both interventions together caused a significant change in gene expression of MuRF1, Atrogin-1, and Myostatin, indicating the significant effect of exercise when combined with BCAA supplementation for Parkinson's disease in rat. Abstract Aim: Parkinson's disease is a chronic neurological disorder that compromises motor and non-motor functions and leads to a decrease in the quality of life and functional capacity of individuals. Therefore, the aim of the present study was to investigate the effect of eight weeks of moderate-intensity endurance training with BCAA supplementation on the gene expression of MuRF1, Atrogin-1, and Myostatin in the soleus muscle of Parkinsonian-induced rats. Methods: In the present experimental study, 25 male Wistar rats, at 12 weeks of age and 180-200 gr were used. The rats were divided into following groups (n=5): healthy control (HC), Parkinson control (PC), Parkinson + training (PT), Parkinson + BCAA (PB), and Parkinson + training + BCAA (PTB) groups. Eight weeks of moderate-intensity endurance training (5 days a week) performed on a treadmill. One-way analysis of variance with the Tukey's post-hoc test used to analyze the data (with a significance level of P≤0.05). Results: The results showed that after Parkinson's induction, genes expression levels of MuRF1, Atrogin-1, and Myostatin in the soleus muscle of rats significantly increased (P=0.001). There was a significant decrease in genes expression of MuRF1, Atrogin-1, and Myostatin (P=0.001) only in the moderate-intensity exercise training with BCAA supplementation group after eight weeks of interventions. Conclusion: Although moderate-intensity endurance training and BCAA supplementation alone did not affect gene expression, combining both interventions together caused a significant change in gene expression of MuRF1, Atrogin-1, and Myostatin, indicating the significant effect of exercise when combined with BCAA supplementation for Parkinson's disease in rat.

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