Azarbaijan Shahid Madani University Journal of Applied Health Studies in Sport Physiology 2676-6507 Articles in Press 2025 12 06 Investigating the Relationship between Spleen Weight and Cancer Morphology in Response to Aerobic Training in Mice with Breast Cancer Investigating the Relationship between Spleen Weight and Cancer Morphology in Response to Aerobic Training in Mice with Breast Cancer 15066 10.22049/jahssp.2025.30529.1736 FA Elham Shahabpour Department of Sport Sciences, Faculty of Humanities Sciences, University of Hormozgan, Bandar Abbas, Iran. Mohammad Amin Delavari , Department of Sport Sciences, Faculty of Humanities Sciences, University of Hormozgan, Bandar Abbas, Iran Babak Ebadi Shir Mard Department of Sport Sciences, Faculty of Humanities Sciences, University of Hormozgan, Bandar Abbas, Iran Journal Article 2025 04 25 Background& purpose: Inflammation plays a role in the development of breast cancer. However, the anti-inflammatory effects of exercise in breast cancer are still unclear. The purpose of the present study was to investigate the relationship between spleen weight and cancer morphology in response to aerobic training in mice with breast cancer. Methods: 20 BALB/c mice were randomly allocated into two groups: tumor-control (n=10) and tumor-training (n=10) groups. The tumor-training group ran on the treadmill for 6 weeks, 5 days a week,Tumor volume, spleen weight, tumor necrosis weight and heart weight were measured at the end. For experiment and data analysis, independent t test and ANOVA test with repeated measurements and Pearson's correlation test at significance level of 0.05 were used. Results: The results showed that the spleen weight of the tumor-control group increased compared to the tumor-training group (p=0.029). Weekly changes in tumor volume showed a significant increase in this variable in the tumor-control group compared to the tumor-training group (p=0.001). Also, the weight of the heart in the tumor-training group showed a significant increase compared to the tumor-control rats (p=0.005). The weight of necrosis in the tumor tissue in the tumor-training group was increased compared to the rats in the tumor-control group (p=0.001). Conclusions This highlights the potential therapeutic value of exercise as a complementary approach to cancer treatment and underscores the importance of understanding the exercise-induced transcriptional changes in the spleen for the development of novel cancer therapies. Background& purpose: Inflammation plays a role in the development of breast cancer. However, the anti-inflammatory effects of exercise in breast cancer are still unclear. The purpose of the present study was to investigate the relationship between spleen weight and cancer morphology in response to aerobic training in mice with breast cancer. Methods: 20 BALB/c mice were randomly allocated into two groups: tumor-control (n=10) and tumor-training (n=10) groups. The tumor-training group ran on the treadmill for 6 weeks, 5 days a week,Tumor volume, spleen weight, tumor necrosis weight and heart weight were measured at the end. For experiment and data analysis, independent t test and ANOVA test with repeated measurements and Pearson's correlation test at significance level of 0.05 were used. Results: The results showed that the spleen weight of the tumor-control group increased compared to the tumor-training group (p=0.029). Weekly changes in tumor volume showed a significant increase in this variable in the tumor-control group compared to the tumor-training group (p=0.001). Also, the weight of the heart in the tumor-training group showed a significant increase compared to the tumor-control rats (p=0.005). The weight of necrosis in the tumor tissue in the tumor-training group was increased compared to the rats in the tumor-control group (p=0.001). Conclusions This highlights the potential therapeutic value of exercise as a complementary approach to cancer treatment and underscores the importance of understanding the exercise-induced transcriptional changes in the spleen for the development of novel cancer therapies. https://jahssp.azaruniv.ac.ir/article_15066_cef565bbf7a035201a6702169ced148d.pdf