Azarbaijan Shahid Madani University Journal of Applied Health Studies in Sport Physiology 2676-6507 Articles in Press 2025 10 13 The effect of eight weeks of moderate-intensity interval training on gene expression of indicators involved in mitochondrial fission and fusion in the liver tissue of male Wistar rats The effect of eight weeks of moderate-intensity interval training on gene expression of indicators involved in mitochondrial fission and fusion in the liver tissue of male Wistar rats 15029 10.22049/jahssp.2025.30593.1746 FA Mehdi Ramezanpoor Department of Exercise Physiology, Faculty of Sport Sciences, Hakim Sabzevari University, Sabzevar, Iran Amir Hossein Haghighi Department of Exercise Physiology, Faculty of Sport Sciences, Hakim Sabzevari University, Sabzevar, Iran Keyvan Hejazi Department of Exercise Physiology, Faculty of Sport Sciences, Hakim Sabzevari University, Sabzevar, Iran Mohammad Reza Bandali Department of Exercise Physiology, Faculty of Sport Sciences, Hakim Sabzevari University, Sabzevar, Iran Hadi Shahrabadi Department of Exercise Physiology, Faculty of Sport Sciences, Hakim Sabzevari University, Sabzevar, Iran Journal Article 2025 05 05 Background& purpose: Mitochondria are dynamic organelles that are constantly undergoing fission and fusion. The purpose of this study was to investigate the effect of eight weeks of moderate-intensity interval training on gene expression of indicators involved in mitochondrial fission and fusion in the liver tissue of male Wistar rats. Methods: In an experimental study, 12 male rats with an average weight of 180 ± 20 g were purchased and included in the study. Then they were placed in two groups of moderate-intensity interval training (n=6) and control (n=6) with the help of simple random method. The moderate intensity interval training was performed for 8 weeks and 5 sessions per week. In the present study, rat liver tissue was used to investigate the genes expression of MFN1, MFN2, OPA1, DRP1, FIS1, PGC-1α and OXR1. Independent t-test and Mann-Whitney were used to compare between groups. Data were analyzed using SPSS version 23 software at a significance level of p<0.05. Results: The results showed that 8 weeks of moderate-intensity interval training did not cause significant changes in body weight, genes expression of MFN1, MFN2, OPA1, DRP1, FIS1, PGC-1α and OXR1 and OPA1/FIS1 ratio in the liver cells of male Wistar rats (p>0.05). Conclusion: It does not seem that moderate-intensity interval training alters the processes of mitochondrial fusion and fission in the liver cells of healthy male Wistar rats. Background& purpose: Mitochondria are dynamic organelles that are constantly undergoing fission and fusion. The purpose of this study was to investigate the effect of eight weeks of moderate-intensity interval training on gene expression of indicators involved in mitochondrial fission and fusion in the liver tissue of male Wistar rats. Methods: In an experimental study, 12 male rats with an average weight of 180 ± 20 g were purchased and included in the study. Then they were placed in two groups of moderate-intensity interval training (n=6) and control (n=6) with the help of simple random method. The moderate intensity interval training was performed for 8 weeks and 5 sessions per week. In the present study, rat liver tissue was used to investigate the genes expression of MFN1, MFN2, OPA1, DRP1, FIS1, PGC-1α and OXR1. Independent t-test and Mann-Whitney were used to compare between groups. Data were analyzed using SPSS version 23 software at a significance level of p<0.05. Results: The results showed that 8 weeks of moderate-intensity interval training did not cause significant changes in body weight, genes expression of MFN1, MFN2, OPA1, DRP1, FIS1, PGC-1α and OXR1 and OPA1/FIS1 ratio in the liver cells of male Wistar rats (p>0.05). Conclusion: It does not seem that moderate-intensity interval training alters the processes of mitochondrial fusion and fission in the liver cells of healthy male Wistar rats. https://jahssp.azaruniv.ac.ir/article_15029_510e59176d1f790415c6e2ca0c2d4ecb.pdf