Azarbaijan Shahid Madani University Journal of Applied Health Studies in Sport Physiology 2676-6507 10 2 2023 09 23 The effect of high-intensity interval training and curcumin supplementation on apoptosis indicators such as TNF-α, nfkb and fas on hippocampal neurotoxicity caused by hydrogen peroxide consumption in male rats The effect of high-intensity interval training and curcumin supplementation on apoptosis indicators such as TNF-α, nfkb and fas on hippocampal neurotoxicity caused by hydrogen peroxide consumption in male rats 54 67 14555 10.22049/jahssp.2023.28273.1543 FA Zahra Toktam Barmar Doctoral student of sports physiology majoring in nerve and muscle, Department of Physical Education and Sports Sciences, Bojnord Branch, Islamic Azad University, Bojnord, Iran Sadegh Cheragh-Birjandi Department of Physical Education, Isalmic Azad University, Bojnourd branch, Bojnourd, Iran Najmeh Rezaeian Department of Physical Education Islamic Azad University, Bojnourd Branch, Bojnourd, Iran Journal Article 2023 02 24 <strong>Aim</strong>:   Apoptosis is a type of programmed death that is important in regulating the balance between cell death and tissue growth. Thus, the aim of this study is to investigate the effect of high-intensity interval training and curcumin supplement on apoptosis indicators such as TNF-α,<strong> </strong>NF-kB<strong> </strong>and Fas on hippocampal neurotoxicity caused by hydrogen peroxide consumption in male rats. <strong>Methods</strong>: This is an experimental research in which 50 adult male Wistar rats weighing 20 ± 200 g and aged 8 weeks were randomly divided into 5 equal groups of saline, hydrogen peroxide (H₂O₂, intense intermittent exercise + oxygenated water, curcumin supplement + hydrogen peroxide and intense intermittent exercise + Curcumin supplementation + hydrogen peroxide (n=10) were divided. H₂O₂in the amount of 1 mmol per kg of body weight 3 times a week on even days and curcumin, 150 mg per kg of body weight per day, was used by gavage. The treadmill running program was performed for 8 weeks (five sessions per week with an intensity of 85-90 % of the maximum speed). At the end of the injection and training period, rats were extracted to evaluate changes in gene expression of hippocampal tissue. Data were analyzed using one-way ANOVA method at a significant level (α≥0.05). <strong>Results</strong>:  The findings showed that hydrogen peroxide injection led to a significant increase in the expression of TNF-α, NF-κB, Fas genes (P=0.001). HIIT exercise and curcumin supplement each alone and together lead to decreasing changes in the expression of TNF-α, NF-κB, Fas (P=0.001), which shows the positive effect of exercise training and curcumin supplementation on the desired indicators.  <strong>Conclusions:</strong> It seems so, exogenous injection of H₂O₂has led to an increased the process of neuronal apoptosis. HIT exercises and curcumin supplement has been able to reduce the process of apoptosis. <strong>Aim</strong>:   Apoptosis is a type of programmed death that is important in regulating the balance between cell death and tissue growth. Thus, the aim of this study is to investigate the effect of high-intensity interval training and curcumin supplement on apoptosis indicators such as TNF-α,<strong> </strong>NF-kB<strong> </strong>and Fas on hippocampal neurotoxicity caused by hydrogen peroxide consumption in male rats. <strong>Methods</strong>: This is an experimental research in which 50 adult male Wistar rats weighing 20 ± 200 g and aged 8 weeks were randomly divided into 5 equal groups of saline, hydrogen peroxide (H₂O₂, intense intermittent exercise + oxygenated water, curcumin supplement + hydrogen peroxide and intense intermittent exercise + Curcumin supplementation + hydrogen peroxide (n=10) were divided. H₂O₂in the amount of 1 mmol per kg of body weight 3 times a week on even days and curcumin, 150 mg per kg of body weight per day, was used by gavage. The treadmill running program was performed for 8 weeks (five sessions per week with an intensity of 85-90 % of the maximum speed). At the end of the injection and training period, rats were extracted to evaluate changes in gene expression of hippocampal tissue. Data were analyzed using one-way ANOVA method at a significant level (α≥0.05). <strong>Results</strong>:  The findings showed that hydrogen peroxide injection led to a significant increase in the expression of TNF-α, NF-κB, Fas genes (P=0.001). HIIT exercise and curcumin supplement each alone and together lead to decreasing changes in the expression of TNF-α, NF-κB, Fas (P=0.001), which shows the positive effect of exercise training and curcumin supplementation on the desired indicators.  <strong>Conclusions:</strong> It seems so, exogenous injection of H₂O₂has led to an increased the process of neuronal apoptosis. HIT exercises and curcumin supplement has been able to reduce the process of apoptosis. https://jahssp.azaruniv.ac.ir/article_14555_a4a332d313222cb784663a1f09f49fa9.pdf