تاثیر سه شیوه‌ی متفاوت تمرینات ورزشی HIIT، HIT و MIT بر بیان ژن های FGF و TGF در بافت کبد رت‌های نر ویستار

نوع مقاله : مقاله پژوهشی Released under CC BY 4.0 license I Open Access I

نویسندگان

1 استادیار،گروه تربیت بدنی ، دانشکده علوم تربیتی و روانشناسی ، دانشگاه پیام نور، تهران ،ایران.

2 ارشد،گروه تربیت بدنی ، دانشکده علوم تربیتی و روانشناسی ، دانشگاه پیام نور، تهران ،ایران.

10.22049/jahssp.2022.27721.1451

چکیده

هدف : هدف از انجام تحقیق حاضر بررسی تاثیر تمرینات ورزشی مختلف بر بیان ژن های FGF و TGF بافت کبد رت­های نر نژاد ویستار بود .روش­ شناسی : بدین منظور تعداد ۳۲ سر رت نر با میانگین وزنی ۲۵۰ گرم تهیه شدند. رت­های نر نژاد ویستار به صورت تصادفی به چهار گروه مساوی کنترل ، تمرین با شدت متوسط، تمرین با شدت بالا  و تمرین تناوبی با شدت بالا تقسیم شدند. گروه های تمرینی به مدت ۸ هفته در برنامه های تمرینی با شدت متوسط ، شدت بالا و تناوبی با شدت  بالا شرکت کردند. ۲۴ ساعت پس از آخرین جلسه تمرین بافت کبد موش ها استخراج و در دمای ۸۰- درجه سانتی گراد نگهداری شد و میزان تغییرات ژن TGF ،FGF با استفاده از روش Real time PCR  تعیین گردید. جهت بررسی تفاوت بین گروهی متغیرهای از آزمون آماری تحلیل واریانس یکطرفه و آزمون تعقیبی توکی استفاده شد. جهت تجزیه و تحلیل داده ها از SPSS-24 استفاده شد. یافته ­ها : نتایج نشان داد اختلاف معنی­داری در بیان TGFوFGF  بین گروه‌ها (کنترل، HIT، HIIT وMIT) وجود داشت (0005/0=p)(001/0=p). نتایج آزمون توکی نشان داد که افزایش معنی داری در بیان ژنTGF در میان گروه های تمرینی(HIT ،MIT ،HIIT) نسبت به گروه کنترل وجود داشت(001/0=P). همچنین افزایش معنی داری در بیان ژن FGF در میان گروه های تمرینی(HIT ،MIT ،HIIT) نسبت به گروه کنترل وجود داشت(001/0=P). نتیجه گیری : در تحقیق حاضر نشان داده شد که سه شیوه تمرینی MIT ، HIT و HIIT باعث افزایش  بیان TGF وFGF  در بافت کبد میشوند اما به نظر می رسد که افزایش بیان این ژنها در تمرینات MIT  بیشتر از HIT و HIIT بوده است. 

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

The effect of three different exercise methods HIIT, HIT and MIT on the expression of FGF and TGF genes in liver tissue of male Wistar rats

نویسندگان [English]

  • Ali Barzegari 1
  • Saeed Naghibi 1
  • Mohammad Hassan Dashty 1
  • Farzad Karimi Zafarabadi 2
1 Assistant Professor, Department of Physical Education, Faculty of Educational Sciences and Psychology, Payame Noor University, Tehran, Iran.
2 Master,, Department of Physical Education, Faculty of Educational Sciences and Psychology, Payame Noor University, Tehran, Iran.
چکیده [English]

Aim: The aim of this study was to investigate the effect of different exercises on the expression of FGF and TGF genes in the liver tissue of male Wistar rats.
Methods: For this purpose, 32 Male Wistar rats with an average weight of 250 g were prepared. Mice randomly divided into four equal groups of control, moderate intensity training, and high intensity training and high intensity intermittent training. The training groups participated in moderate-intensity, high-intensity, and high-intensity interval training programs for 8 weeks. 24 hours after the last training session, rat liver tissue was extracted and stored at -80 ° C and the amount of TGF and FGF gene changes was determined using Real time PCR. One-way analysis of variance and Tukey's post hoc test used to examine the differences between groups of variables. SPSS-24 used for data analysis.
Results:  The results showed that there was a significant difference in the expression of TGF and FGF between the groups (control, HIT, HIIT and MIT) (p = 0.0005) (p = 0.001).The results of Tukey test showed that there was a significant increase in TGF gene expression among training groups (HIT, MIT, HIIT) compared to the control group (P = 0.001)In addition,  There was also a significant increase in FGF gene expression among training groups (HIT, MIT, HIIT) compared to the control group (P = 0.001).
Conclusion: In the present study, it was shown that three training methods, MIT, HIT and HIIT, increase the expression of TGF and FGF in liver tissue, but it seems that the expression of these genes in MIT training was more than HIT and HIIT.

کلیدواژه‌ها [English]

  • Different training methods
  • TGF
  • FGF
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دوره 9، شماره 1
فروردین 1401
صفحه 100-113
  • تاریخ دریافت: 26 اسفند 1400
  • تاریخ بازنگری: 22 اردیبهشت 1401
  • تاریخ پذیرش: 24 اردیبهشت 1401
  • تاریخ اولین انتشار: 24 اردیبهشت 1401