Effect of Resistance Training Combined with Curcumin Supplementation on Expression of Regulatory Genes Related to Myocardial Remodeling in Obese Rats

Document Type : Research Paper I Open Access I Released under (CC BY-NC 4.0) license


1 Ph.D Student in Exercises Physiology, Department of Physical Education and Sport Sciences, Sport Physiology Department, Islamic Azad University, Larestan Branch, Larestan, Iran

2 Associate Professor in exercises Physiology, Department of exercises Physiology, Islamic Azad University, Marvdasht Branch, Marvdasht, Iran



Aim: Cardiac aging is a major consequence of obesity that results in pathologic cardiac hypertrophy. However, resistance training and curcumin polyphenols can modulate some of involved intracellular signaling pathways. Therefore, the aim of this study was to investigate the effect of resistance training and curcumin supplementation on expression of regulatory genes involved in cardiac structural remodeling. Methods: in this experimental study, 18 male Sprague Dawley rats were divided into Normal Weight Control (n=6), Obese Reference (n = 6), Training + Curcumin (n = 6) groups following to eight weeks of high fat diet consumption. Resistance training were conducted also for eight weeks, three sessions per week against 20 to 50% of body weight. Curcumin (150 mg per kg body weight, daily) was consumed simultaneously through gavage. The expression levels of AMPK, mTOR, S6K, 4EBP, COL1, COL3 and AngII genes were assessed using Real Time-PCR technique and thy data was analyzed by One-way ANOVA. Results: Obesity was lead to AMPK gene expression level downregulation, while expression levels of mTOR, S6K, 4EBP, COL1, COL3, AngII genes were up-regulated (p < 0.05). However; there were also significant differences in expression levels of these genes between Training + Curcumin and Obese Reference groups (p < 0.05). Conclusion: It seems obesity causes acceleration of the processes involved in cardiac pathologic hypertrophy, while resistance training with curcumin supplementation could decrease this hazards to some extent.


1.            Harman D. Role of antioxidant nutrients in aging: overview. Age. 1995;18(2):51-62.
2.            Dai D-F, Chen T, Johnson SC, Szeto H, Rabinovitch PS. Cardiac aging: from molecular mechanisms to significance in human health and disease. Antioxidants & redox signaling. 2012;16(12):1492-526.
3.            Chiao YA, Rabinovitch PS. The aging heart. Cold Spring Harbor perspectives in medicine. 2015;5(9):a025148.
4.            Johnson SC, Sangesland M, Kaeberlein M, Rabinovitch PS. Modulating mTOR in aging and health.  Aging and Health-A Systems Biology Perspective. 40: Karger Publishers; 2015. p. 107-27.
5.            Wessells R, Fitzgerald E, Piazza N, Ocorr K, Morley S, Davies C, et al. d4eBP acts downstream of both dTOR and dFoxo to modulate cardiac functional aging in Drosophila. Aging cell. 2009;8(5):542-52.
6.            Saxton RA, Sabatini DM. mTOR signaling in growth, metabolism, and disease. Cell. 2017;168(6):960-76.
7.            Baar K, Wende AR, Jones TE, Marison M, Nolte LA, Chen M, et al. Adaptations of skeletal muscle to exercise: rapid increase in the transcriptional coactivator PGC‐1. The FASEB journal. 2002;16(14):1879-86.
8.            Goodman CA, Frey JW, Mabrey DM, Jacobs BL, Lincoln HC, You JS, et al. The role of skeletal muscle mTOR in the regulation of mechanical load‐induced growth. The Journal of physiology. 2011;589(22):5485-501.
9.            Pesta D, Hoppel F, Macek C, Messner H, Faulhaber M, Kobel C, et al. Similar qualitative and quantitative changes of mitochondrial respiration following strength and endurance training in normoxia and hypoxia in sedentary humans. American Journal of Physiology-Regulatory, Integrative and Comparative Physiology. 2011;301(4):R1078-R87.
10.          Khodaparast Z, Yousofi A, Khoshvaghti A. Investigation of Curcumin Effects on Liver Tissue in Adult Male Rats Treated with Cyclophosphamide. Journal of Fasa University of Medical Sciences/Majallah-i Danishgah-i Ulum-i Pizishki-i Fasa. 2014;4(4): 11-19.
11.          Boon H, Wong J. Botanical medicine and cancer: a review of the safety and efficacy. Expert opinion on pharmacotherapy. 2004;5(12):2485-501.
12.          Kapakos G, Youreva V, Srivastava AK. Cardiovascular protection by curcumin: molecular aspects. Indian J Biochem Biophys. 2012;49(5):306-15.
13.          Shen L, Ji H-F. Bidirectional interactions between dietary curcumin and gut microbiota. Critical reviews in food science and nutrition. 2019;59(18):2896-902.
14.          Moeini A, Farsi S, Moghaddasi M. The Effect of Curcumin Supplementation on Expression of Regulatory Signaling Genes of Cardiac Muscle Growth Messenger in Obese Male Rats. Journal of Arak University of Medical Sciences. 2019;22(2):96-105.
15.          Rauofi A, Farsi S, Hosseini SA. Effect of Resistance Training Along with Curcumin Supplementation on Expression of Some Regulator Genes Associated with Cardiac Muscle Structure in Obese Rats. Thrita. 2020;9(2): 27-35.
16.          Salesi M, Mehrtash M, Daryanoosh F, Tanide N. The Role of Caloric Restriction on Lipid Coat Proteins Gene Expression and Insulin Resistance after 8 Weeks High Caloric Diet in Male Rats. Journal of Arak University of Medical Sciences. 2018;21(5):21-31.
17.          Miyamoto S. Autophagy and cardiac aging. Cell Death & Differentiation. 2019;26(4):653-64.
18.          Perl A. mTOR activation is a biomarker and a central pathway to autoimmune disorders, cancer, obesity, and aging. Annals of the New York Academy of Sciences. 2015;1346(1):33-41.
19.          Sun M, Tan Y, Rexiati M, Dong M, Guo W. Obesity is a common soil for premature cardiac aging and heart diseases-role of autophagy. Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease. 2019;1865(7):1898-904.
20.          Fang CX, Dong F, Thomas DP, Ma H, He L, Ren J. Hypertrophic cardiomyopathy in high-fat diet-induced obesity: role of suppression of forkhead transcription factor and atrophy gene transcription. American Journal of Physiology-Heart and Circulatory Physiology. 2008;295(3):H1206-H15.
21.          Bujak M, Frangogiannis NG. The role of TGF-β signaling in myocardial infarction and cardiac remodeling. Cardiovascular research. 2007;74(2):184-95.
22.          Lakshmipathi J, Alvarez-Perez JC, Rosselot C, Casinelli GP, Stamateris RE, Rausell-Palamos F, et al. PKCζ is essential for pancreatic β-cell replication during insulin resistance by regulating mTOR and cyclin-D2. Diabetes. 2016;65(5):1283-96.
23.          Phan F, Flamment M, Hu M, Hainault I, Ferré P, Foufelle F. BCAA and ER stress activate SREBP-1c cleavage and hepatic lipogenesis through mTOR. Journal of Hepatology. 2018;68:S339.
24.          Shen M, Schmitt S, Buac D, Dou QP. Targeting the ubiquitin–proteasome system for cancer therapy. Expert opinion on therapeutic targets. 2013;17(9):1091-108.
25.          Zhai X, Qiao H, Guan W, Li Z, Cheng Y, Jia X, et al. Curcumin regulates peroxisome proliferator-activated receptor-γ coactivator-1α expression by AMPK pathway in hepatic stellate cells in vitro. European journal of pharmacology. 2015; 746: 56-62.
26.          Li H-L, Liu C, De Couto G, Ouzounian M, Sun M, Wang A-B, et al. Curcumin prevents and reverses murine cardiac hypertrophy. The Journal of clinical investigation. 2008;118(3):879-93.
27.          Marcu MG, Jung Y-J, Lee S, Chung E-J, Lee M-J, Trepel J, et al. Curcumin is an inhibitor of p300 histone acetylatransferase. Medicinal chemistry. 2006;2(2):169-74.
28.          Moini A, Farsi S, Hoseini S, Mehrzad M. The Effect of Resistance Training on the Expression of Cardiac Muscle Growth Regulator Messenger Genes in Obese Male Rats. Armaghane danesh. 2019;24(5):935-49.
29.          Balakrishnan VS, Rao M, Menon V, Gordon PL, Pilichowska M, Castaneda F, et al. Resistance training increases muscle mitochondrial biogenesis in patients with chronic kidney disease. Clinical Journal of the American Society of Nephrology. 2010;5(6):996-1002.
30.          Choobineh S, Soleimani M, Shafiee A, Hadidi V. The Effect Of Eight Week Continuous Training On Expression Of Mir29mRNA, In Healthy Male Rat’s Cardiac Muscle. Journal of Shahrekord Uuniversity of Medical Sciences. 2016;17(3): 21-29.