بررسی تاثیر مکمل گیری اسپیرولینا همراه با تمرین مقاومتی غیرخطی بر آنزیم‌های کبدی زنان چاق

نوع مقاله : مقاله پژوهشی Released under CC BY 4.0 license I Open Access I

نویسندگان

1 استادیار، گروه تربیت بدنی و علوم ورزشی، واحد لار، دانشگاه آزاد اسلامی لارستان، لار، ایران

2 استادیار، دانشکده علوم انسانی، دانشگاه آزاد اسلامی لامرد، لامرد، ایران

10.22049/jassp.2020.14017

چکیده

هدف: چاقی با بیماری‌های مختلفی از جمله قلبی عروقی، دیابت، فشارخون و نیز کبد چرب همراه است که تهدید کننده جدی سلامتی و عامل بسیاری از مرگ و میرها در سراسر جهان هستند. هدف از پژوهش حاضر، بررسی تأثیر یک دوره مکمل‌گیری اسپیرولینا همراه با تمرین مقاومتی غیرخطی بر آنزیم‌های کبدی زنان چاق بود. روش ­شناسی: از طریق روش نمونه­ گیری هدفمند در دسترس 40 زن چاق انتخاب شدند و به طور تصادفی در چهار گروه 10 نفری شامل تمرین مقاومتی غیرخطی، مکمل اسپیرولینا، تمرین مقاومتی غیرخطی + مکمل اسپیرولینا و دارونما قرار گرفتند. گروه مکمل و دارونما به ترتیب روزانه 2 عدد کپسول 500 میلی‌گرمی اسپیرولینا یا نشاسته در وعده های صبح و عصر مصرف کردند. مداخلات به مدت 8 هفته انجام شد. 24 ساعت قبل و 48 ساعت بعد از پایان مداخلات، از هر چهار گروه در وضعیت 12 ساعت ناشتایی نمونه خونی گرفته شد. برای هر نمونه سطوح AST، ALT و ALP اندازه‌گیری شد. جهت مقایسه و بررسی تغییرات متغیرها در چهار گروه پژوهش و در دو زمان خون‌گیری، از آزمون آماری تحلیل واریانس مدل آمیخته بین- درون‌گروهی در یک طرح 2×4 استفاده شد.یافته ­ها: نتایج نشان داد تفاوت معنی­­داری در  میزان آنزیم‌های AST (p=0/25)، ALT (p=0/92) و ALP (p=0/76) بین گروه­ها وجود نداشت. نتیجه­ گیری: با توجه به نتایج این پژوهش احتمالا می ­توان گفت هشت هفته تمرین مقاومتی غیرخطی و مصرف مکمل اسپیرولینا اثری بر آنزیم‌های کبدی زنان چاق ندارد و در نتیجه باعث بهبود نشانگران آنزیمی عملکرد کبد آن‌ها نمی­شود. کافی نبودن طول دوره تمرین و مصرف مکمل و البته عدم کنترل دقیق رژیم غذایی شاید از دلایل عدم بهبود باشد.

کلیدواژه‌ها


عنوان مقاله [English]

Effects of Spirulina Supplementation and Nonlinear Resistance Training on Liver Enzymes in Obese Women

نویسندگان [English]

  • Sirous Farsi 1
  • Hadi Ghaedi 2
1 Assistant Professor, Department of Physical Education and Sport Sciences, larestan Branch, Islamic Azad University, lar Iran
2 Assistant Professor, Faculty of Humanities, Islamic Azad University, Lamerd branch, Lamerd, Iran
چکیده [English]

Aim: Obesity is associated with a variety of diseases, including cardiovascular disease, diabetes, hypertension and fatty liver, which are a serious health threat and cause many deaths worldwide. The purpose of this study was to investigate the effect of a spirulina supplementation course with nonlinear resistance training on liver enzymes in obese women. Methods: Forty obese women in Parsian city were selected through Purposive sampling method and were randomly divided into four groups of 10 people including nonlinear resistance exercise, spirulina supplement, nonlinear resistance exercise + spirulina supplement and placebo. The supplement and placebo group consumed 2 capsules of spirulina daily or starch in the morning and evening meals, respectively. Interventions were performed for 8 weeks. Twenty-four hours before and 48 hours after the intervention, blood samples were taken from all four groups at 12 hours fasting. For each sample, AST, ALT and ALP levels were measured. To compare and evaluate the differences between variables in study groups and at two blood sampling times, 4 * 2 mixed design ANOVA was used.  Results: The results showed that there was no significant difference in AST (p = 0.25), ALT (p = 0.92) and ALP (p = 0.76) enzymes between groups.  Conclusions: According to the results of this study, it can probably be concluded that eight weeks of nonlinear resistance training and spirulina supplementation have no effect on the liver enzymes in obese women and thus do not improve the enzymatic markers of their liver function. Inadequate training and supplement intake and lack of proper diet control may be the reason for lack of improvement.

کلیدواژه‌ها [English]

  • resistance training
  • Spirulina
  • Liver
  • Obesity
 
1.Fan J-G, Kim S-U, Wong VW-S. New trends on obesity and NAFLD in Asia. Journal of Hepatology. 2017;67(4):862-873.
2.Organization WH. Fact sheet: Dengue and severe dengue, 2017 (http://www. who. int/mediacentre/factsheets/fs117/en/). Accessed; 2017.
3.Younossi ZM, Otgonsuren M, Henry L, Venkatesan C, Mishra A, Erario M, et al. Association of nonalcoholic fatty liver disease (NAFLD) with hepatocellular carcinoma (HCC) in the United States from 2004 to 2009. Hepatology. 2015;62(6):1723-1730.
4. Bedogni G, Miglioli L, Masutti F, Tiribelli C, Marchesini G, Bellentani S. Prevalence of and risk factors for nonalcoholic fatty liver disease: the Dionysos nutrition and liver study. Hepatology. 2005;42(1):44-52.
5.Limdi J, Hyde G. Evaluation of abnormal liver function tests. Postgraduate medical journal. 2003;79(932):307-312.
6. Jorkesh Morteza, Ebadi Habib. Effect of six weeks endurance, resistance and combined endurance-resistance training on liver ALT and AST enzymes in ovariectomized rat. Journal of Applied Health Studies in Sport Physiology. 2019; 6(1): 78-85.
7.Zelber‐Sagi S, Nitzan‐Kaluski D, Goldsmith R, Webb M, Zvibel I, Goldiner I, et al. Role of leisure‐time physical activity in nonalcoholic fatty liver disease: a population‐based study. Hepatology. 2008;48(6):1791-1798.
8.Angulo P. Nonalcoholic fatty liver disease. New England Journal of Medicine. 2002;346(16):1221-1231.
9.Aoi W, Ichiishi E, Sakamoto N, Tsujimoto A, Tokuda H, Yoshikawa T. Effect of exercise on hepatic gene expression in rats: a microarray analysis. Life sciences. 2004;75(26):3117-3128.
10.Gholami N, Salekzamani Y, Nahandi MZ, Sokhtehzari S, Monazami AH, Nejad MR. The effect of aerobic exercise on serum level of liver enzymes and liver echogenicity in patients with non alcoholic fatty liver disease. Gastroenterology and Hepatology from bed to bench. 2013;6:2013-6 (Suppl. 1): Gastroenterol Hepatol Bed Bench ; 6 (Suppl. 1): S112-S116.
11.Nikseresht M, Hafezi Ahmadi MR, Hedayati M. Detraining-induced alterations in adipokines and cardiometabolic risk factors after nonlinear periodized resistance and aerobic interval training in obese men. Applied Physiology, Nutrition, and Metabolism. 2016;41(10):1018-1025.
12.Fleck S. Non-linear periodization for general fitness & athletes. Journal of human kinetics. 2011;29(Special Issue):41-45.
13.Kraemer WJ, Fleck SJ. Optimizing strength training: designing nonlinear periodization workouts: Human Kinetics; 2007.
14. Solimani Shahram, Tofighi Asghar, Babaei Solmaz. Effect of 6 weeks aerobic training accompanied by dietary supplementation of spirulina on Oxidative stress index in obese inactive men followed by one session exhaustive exercise. Biannual Journal of Applied Health Studies in Sport Physiology. 2018; 5(2): 36-44.
15.Agrawal A. Pharmacological activities of flavonoids: a review. International journal of pharmaceutical sciences and nanotechnology. 2011;4(2):1394-1398.
16.Szulinska M, Gibas-Dorna M, Miller-Kasprzak E, Suliburska J, Miczke A, Walczak-Gałezewska M, et al. Spirulina maxima improves insulin sensitivity, lipid profile, and total antioxidant status in obese patients with well-treated hypertension: A randomized double-blind placebo-controlled study. Eur Rev Med Pharmacol Sci. 2017;21(10):2473-2481.
17.El-Tantawy WH. Antioxidant effects of Spirulina supplement against lead acetate-induced hepatic injury in rats. Journal of traditional and complementary medicine. 2016;6(4):327-331.
18. Bhatia KK, Puri S, Kaur R, Ahluwalia KK, Ahluwalia AS. Efficacy of Spirulina as Hepatoprotectant: A Review. Vegetos-An International Journal of Plant Research. 2016;29(special):129-136.
19. Wu Q, Liu L, Miron A, Klímová B, Wan D, Kuča K. The antioxidant, immunomodulatory, and anti-inflammatory activities of Spirulina: an overview. Archives of toxicology. 2016;90(8):1817-1840.
20. Pak W, Takayama F, Mine M, Nakamoto K, Kodo Y, Mankura M, et al. Anti-oxidative and anti-inflammatory effects of spirulina on rat model of non-alcoholic steatohepatitis. Journal of clinical biochemistry and nutrition. 2012:12-18.
21.Parikh P, Mani U, Iyer U. Role of Spirulina in the control of glycemia and lipidemia in type 2 diabetes mellitus. Journal of Medicinal Food. 2001;4(4):193-199.
22.Samuels R, Mani U, Iyer U, Nayak U. Hypocholesterolemic effect of Spirulina in patients with hyperlipidemic nephrotic syndrome. Journal of medicinal food. 2002;5(2):91-96.
23. Moura LPd, Gurjão ALD, Jambassi Filho JC, Mizuno J, Suemi C, Mello MARd. Spirulina, exercise and serum glucose control in diabetic rats. Arquivos Brasileiros de Endocrinologia & Metabologia. 2012;56(1):25-32.
24.Lu J, Yang Y, Chen L, Ren D, Cai M, Wang J, et al. In vivo antihypertensive effect of Val-Glu-Pro in spontaneously hypertensive rats. Prog Biochem Biophys. 2011;38:353-360.
25. Lee EH, Park J-E, Choi Y-J, Huh K-B, Kim W-Y. A randomized study to establish the effects of spirulina in type 2 diabetes mellitus patients. Nutrition Research and Practice. 2008;2(4):295-300.
26. Anitha L, Chandralekha K. Effect of supplementation of Spirulina on blood glucose, glycosylated hemoglobin and lipid profile of male non-insulin dependent diabetics. Asian Journal of Experimental Biological Sciences. 2010;1(1):36-46.
27. Park H-J, Lee H-S. The influence of obesity on the effects of spirulina supplementation in the human metabolic response of Korean elderly. Nutrition research and practice. 2016;10(4):418-423.
28. Barzegarzadeh-Zarandi H, Dabidy-Roshan V. Changes in some liver enzymes and blood lipid level following interval and continuous regular aerobic training in old rats. J Shahrekord Univ Med Sci. 2012; 14 (5) :13-23.[In Persian]
29.Kim HJ, Lee YH, Kim CK. Biomarkers of muscle and cartilage damage and inflammation during a 200 km run. European journal of applied physiology. 2007;99(4):443-447.
30. Cinar K, Coban S, Idilman R, Tuzun A, Sarioglu M, BEKTAS M, et al. Long‐term prognosis of nonalcoholic fatty liver disease: Is pharmacological therapy actually necessary? Journal of gastroenterology and hepatology. 2006;21(1):169-173.
31. Moradi Kelardeh B, Keshavarz S, Karimi M. Effects of Nonlinear Resistance Training with Curcumin Supplement on Liver Enzymes in Men with Non-Alcoholic Fatty Liver Disease. Report of Health Care. 2017;3(1):1-9.
32.Baharak Moradi Kelardeh, Mohammad Ali Azarbayjan, Maghsoud Peeri, Hasan matin homaee4. Effects of Nonlinear Resistance Training on Liver Biochemical Marker Levels in Postmenopausal Women with Nonalcoholic Fatty Liver Disease. J Rehab Med. 2017; 5(4): 136-145. .[In Persian]
33.Lee S, Bacha F, Hannon T, Kuk JL, Boesch C, Arslanian S. Effects of aerobic versus resistance exercise without caloric restriction on abdominal fat, intrahepatic lipid, and insulin sensitivity in obese adolescent boys: a randomized, controlled trial. Diabetes. 2012;61(11):2787-2795.
34. Lee S, Deldin AR, White D, Kim Y, Libman I, Rivera-Vega M, et al. Aerobic exercise but not resistance exercise reduces intrahepatic lipid content and visceral fat and improves insulin sensitivity in obese adolescent girls: a randomized controlled trial. American journal of physiology-endocrinology and metabolism. 2013;305(10):E1222-E9.
35. Ahmadian T, Tofighi A. The effect of 6-Week aerobic exercises with the spirulina supplementation consumption on aerobic performance in non-athletic girls. 3. 2016; 8 (15) :37-43.[In Persian]
36. Ghaedi Hadi, Farsi Siros, Taghipour Asrami Amir, Kaka Raheleh. The Effect of Nonlinear Resistance Training with Supplementation of Spirulina on serum Leptin and Ghrelin in obese women. Journal of Applied Health Studies in Sport Physiology. 2019; 6(1): 69-77.[In Persian]
37. Barani F, Afzalpour ME, Ilbiegi S, Kazemi T, Mohammadi Fard M. The effect of resistance and combined exercise on serum levels of liver enzymes and fitness indicators in women with nonalcoholic fatty liver disease. Journal of Birjand University of Medical Sciences. 2014;21(2):188-202.
38. Ranjbar K, Nazem F, Hashemi S. Effect of Continuous Aerobic Training on Serum Levels of Liver Injury Indices in Rats with Myocardial Infarction. 2016;18 (1):46-53.[In Persian]
39.Slentz CA, Bateman LA, Willis LH, Shields AT, Tanner CJ, Piner LW, et al. Effects of aerobic vs. resistance training on visceral and liver fat stores, liver enzymes, and insulin resistance by HOMA in overweight adults from STRRIDE AT/RT. American Journal of Physiology-Endocrinology and Metabolism. 2011;301(5):E1033-E9.
40. Bemben D, Palmer I, Abe T, Sato Y, Cramer J, Bemben M. Effects of a Single Bout of Low Intensity KAATSU Resistance Training on Markers of Bone Turnover in Men: 2754Board# 28 8: AM–9: AM. Medicine & Science in Sports & Exercise. 2006;38(5):S531.
41. Hallsworth K, Fattakhova G, Hollingsworth KG, Thoma C, Moore S, Taylor R, et al. Resistance exercise reduces liver fat and its mediators in non-alcoholic fatty liver disease independent of weight loss. Gut. 2011;60(9):1278-1283.
42. de Piano A, de Mello MT, Sanches PdL, da Silva PL, Campos RM, Carnier J, et al. Long-term effects of aerobic plus resistance training on the adipokines and neuropeptides in nonalcoholic fatty liver disease obese adolescents. European journal of gastroenterology & hepatology. 2012;24(11):1313-1324.
43. Alie M, Matinhommaee H, Azarbayjani M, Peeri M. The effect of resistance training intensity on enzymatic and nonenzymatic markers of liver function in obese males. Ind J Fund Appl Life Sci. 2015;5(2):101-110.
44. Nabizadeh Haghighi A, Shabani R. Comparing Effects of Medication Therapy and Exercise Training with Diet on Liver enzyme Levels and Liver Sonography in Patients with Non-Alcoholic Fatty Liver Disease (NAFLD). J Fasa Univ Med Sci. 2016; 5 (4) :488-500.[In Persian]
45. Tartibian B, MOUTAB SN. Effects of 9-weeks high intensity aerobic exercises on parathyroid hormone and marker of metabolism of bone formation in young women. 2009; 16(4):79-88.[In Persian]
46. Vakili J, Sasan RA, Ordibazar F. Effect of 8 weeks endurance training with Chlorella Vulgaris supplementation on liver enzymes levels in women with type 2 diabetes. Majallah-i pizishki-i Danishgah-i Ulum-i Pizishki va Khadamat-i Bihdashti-i Darmani-i Tabriz. 2019;40(6):88-97.
47. Mougios V. Exercise biochemistry: Human Kinetics Publishers; 2019.
48. Bashiri J, Hadi H, Bashiri M, Nikbakht H, Gaeini A. Effect of concurrent creatine monohydrate ingestion and resistance training on hepatic enzymes activity levels in non-athlete males. Iranian journal of endocrinology and metabolism. 2010;12(1):42-47.[In Persian]
49. Martínez AC, Villa G, Aguiló A, Tur JA, Pons A. Hand strike-induced hemolysis and adaptations in iron metabolism in Basque ball players. Annals of nutrition and metabolism. 2006;50(3):206-213.
50. Bagherieh hagh H S, Nasri S, Kerishchi Khiabani P. The Effects Of Romarinus Officinalis Leaf Hydroalcoholic Extract On Histopathology And Enzymes Activity Of Liver In Alloxan Induced Diabetic Rats. ijdld. 2018; 17 (4) :189-197.[In Persian]
51. Fathi m, Khairabadi s, Ramezani f, Hejazi k. Histopathology And Enzymes Activity Of Liver In Alloxan Induced Diabetic Rats. Iranian Journal of Diabetes and Metabolism. 2018;17(4):189-97. .[In Persian]
52. Burtis C, Ashwood E, Bruns D. Clinical biochemistry tietz: analyte and pathophysiology. translate by: Amirrasouli H. Tehran: Ketab Arjmand publication; 2011. p26.
53. Fan R, Wang J, Du J. Association between body mass index and fatty liver risk: A dose-response analysis. Scientific reports. 2018;8(1):1-7.
54. Brea A, Puzo J. Non-alcoholic fatty liver disease and cardiovascular risk. International journal of cardiology. 2013 Aug 20;167(4):1109-1117.
55. Hooshmand B, Attarzade Hosseini S, Kordi M, Davaloo T. The effect of 8-week aerobic exercise with spirulina supplementation consumption on plasma levels of MDA, SOD and TAC in men with type 2 diabetes. Sport Physiol Manag Investig. 2018;10(4):139-148.[In Persian]
 
 
 
 
 
 
 
 
دوره 7، شماره 1
فروردین 1399
صفحه 55-64
  • تاریخ دریافت: 02 اردیبهشت 1399
  • تاریخ بازنگری: 08 خرداد 1399
  • تاریخ پذیرش: 19 خرداد 1399
  • تاریخ اولین انتشار: 19 خرداد 1399