تاثیر تمرین تداومی و مکمل سویا بر بیان ژن mir-29 در قلب رت های اواریکتومی شده

نوع مقاله: مقاله پژوهشی

نویسندگان

1 دانشجوی دکترای فیزیولوژی ورزشی قلب و عروق و تنفس، گروه فیزیولوژی ورزشی، دانشکده تربیت بدنی و علوم ورزشی، دانشگاه تهران، پردیس بین المللی ارس، تهران، ایران

2 دانشیار گروه فیزیولوژی ورزشی، دانشکده تربیت بدنی و علوم ورزشی، دانشگاه تهران، تهران، ایران

3 استاد گروه فیزیولوژی ورزشی، دانشکده تربیت بدنی و علوم ورزشی، دانشگاه تهران، تهران، ایران

10.22049/jassp.2019.26577.1243

چکیده

بیماری های قلبی مهمترین عامل مرگ و میر زنان یائسهدردنیا تلقی می شوند. هدف از این مطالعه بررسی تاثیر تمرین تداومی و مکمل سویا بر  بیان ژن mir-29 در قلب رت های اواریکتومی می باشد. تعداد 40 سر موش صحرایی ماده، نژاد ویستار بطور تصادفی انتخاب شده و پس از جراحی اواریکتومی به 5 گروه تقسیم شدند. تمرین تداومی و مکمل سویا به مدت 6 هفته استفاده شد. پس از 24 ساعت از آخرین جلسه تمرین، قلب رت ها به آزمایشگاه منتقل شد. میزان بیان ژن mir-29 به روش RT- PCR سنجیده شد. تجزیه و تحلیل داده ها با استفاده از آنالیز واریانس یکطرفه و آزمون تعقیبی توکی انجام شد. اواریکتومی باعث کاهشبیان ژن mir-29 شد، ولی بین آثار تمرین تداومی و ترکیب تمرین و مکمل سویا بر بیان ژن mir-29 در قلب رت های اواریکتومی تفاوت معناداری مشاهده نشد(P>0.05). تمرین و مکمل سویا از کاهش بیان ژن mir-29 جلوگیری کردند.هر چند این تاثیر معنادار نبود.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Effect continuous training and soya supplement on the expression of the mir-29 gene in the heart of ovariectomized rats

نویسندگان [English]

  • Lamia Mir Heidari 1
  • Rahman Souri 2
  • Ali Asghar Ravasi 3
1 Ph.D. student of Physiology of Cardiovascular and Breathing Physiology, Department of Sport Physiology, Faculty of Physical Education and Sport Sciences, University of Tehran, Aras International Campus, Tehran, Iran
2 Associate Professor of Sport Physiology, Faculty of Physical Education and Sport Sciences, University of Tehran, Tehran, Iran.
3 Professor of Sport Physiology, Faculty of Physical Education and Sport Sciences, University of Tehran, Tehran, Iran.
چکیده [English]

Cardiovascular disease is the most important cause of death in women in the world. The purpose of this study was to determine the effect of continuous training and soya on the expression of the mir-29 gene in the heart of the ovariectomized rats. 40 Wistar rats were randomly selected and divided into 5 groups after ovariectomy surgery. Continuous exercise and soya supplementation were done for 6 weeks. After 24 hours of the last workout, the heart is transferred to the lab. The amount of expression of the mir-29 gene in the method RT-PCR was measured. Data analysis was performed using one-way ANOVA and Tukey's post hoc test. Ovariectomy reduced the expression of the mir-29 gene expression, however, there was no significant difference between the effects of continuous training, combinated training and supplementary soya on the mir-29 gene in the heart of the ovariectomized rats (P>0.05). Exercise and soya supplementation non significantly prevented the reduction of the expression of the mir-29 gene.

کلیدواژه‌ها [English]

  • Continuous exercise
  • soya supplement
  • mir-29 erythema

 

1.       M, Nguyen K, Miao W, Garantziotis S, Factor SM, et al. A mechanistic role for cardiac myocyte apoptosis in heart failure. J Clin Invest 2003 ; 111:1497 – 504.

2.       Van Rooij E, Liu N, Olson EN. MicroRNAs flex their muscles. Trends in Genetics 2008 ; 24(4):159-66.

3.       VaccarinoV. Angina and Cardiac Car: Are There Gender Differences and If So,Why? 2006 ; 113: 467 – 469

4.       Muñoz-Castañeda JR, Muntané J, Herencia C,et al. Ovariectomy exacerbates oxidative stressand cardiopathy induced by Adriamycin. GynecolEndocrinol2006; 22:74-79.

5.       Underwood J, Jordan ML, Lorenz L, et.The role of emergency Nurs in Improving Care of the Patient. Journal of Emergency Nursing 2009; 35(4): 330-335.

6.       Bitto A, Altavilla D, Bonaiuto A, Polito F, Minutoli L, Di Stefano V, Giuliani D,Guarini S, Arcoraci V, Squadrito F: Effects of aglycone genistein in a rat experimental model of  postmenopausal metabolic syndrome.J Endocrinol 2009; 200:367–376.

7.       Rosano GM, Vitale C, Fini M. Cardiovascular aspects of menopausal hormone replacement therapy. Climacteric 2009; 12 (Suppl 1):41–46.

8.       Van Rooij E, Quiat D, Johnson BA, Sutherland LB, Qi X, Richardson JA, et al. A family of microRNAs encoded by myosin genes governs myosin expression and muscle performance. Dev Cell 2009;17(5):662-7

9.       Durrans A, Stuhlmann H. A role for Egfl7 during endothelial organization in the embryoid body model system. J Angiogenes Res 2010; 2: 4. doi:10.1186/2040-2384-2-4. 

10.    Soci UPR, Fernandes T, Hashimoto NY, Mota GF, Amadeu MA, Rosa KT, et al. MicroRNAs 29 are involved in the improvement of ventricular compliance promoted by aerobic exercise training in rats. Physiological genomics 2011; 43(11):665-73.

11.    Melo SF, Fernandes T, Baraúna V, Matos KC, Santos AA, Tucci PJ, et al. Expression of microRNA-29 and collagen in cardiac muscle after swimming training in myocardial-infarcted rats. Cellular Physiology and Biochemistry 2014; 33(3):657-69.

12.    Habibi P, Alihemmatti A, Alipour M, Nourazar A, Yousefi H, Andalib S, Ahmadiasl N.  Effects of Exercise on miR-29 and IGF-1 Expression and Lipid Profile in the Heart of Ovariectomized Rat. Acta Endo (Buc) 2016; 12 (2): 130-136.

13.    Jourkesh M , Soori R , Ravasi A,  Stephen R, el. Effects of six weeks endurance-resistance training on microRNA-29 Expression in the Heart of Ovariectomized Rat.  

14.    Fernandes T, Soci UPR, Oliveira EM (2011). Eccentric and concentric cardiac hypertrophyinduced by exercise training: microRNAs andmolecular determinants. J Med Biologic Res.; 44: 836-47.

15.    Soci UPR, Fernandes T, Hashimoto NY, Mota GF, Amadeu MA, Rosa KT, et al. MicroRNAs 29 are involved in the improvement of ventricular compliance promoted by aerobic exercise training in rats. Physiological genomics 2011 ; 43(11):665-73.

16.    Nekoyi A, Kordi M R , Choobine S. effect of 8 weeks HIT training  on expression of MicroRNAs- 29 genes in the heart of rat.J Shahre kord Medicin 2017;(17) 113-120

17.    Lawier, J. M., Powers, S. K., Hammere, J., Martin, A. D. Oxigen costof treadmill running in 24- month-oldfisheher-344 rats: Medsi sportsexer 1993; 25(11), 1259-1264.

18.    Jaetaek Kim,et a. Insulin-Like Growth Factor I Receptor Signaling Is Required for Exercise-Induced Cardiac Hypertrophy. Molecular Endocrinology 2013; 11:1-11.

19.    Teoman, N., Ozcan, A., & Acar, B. The effect of exercise on physical fitness and quality of life in postmenopausal women. Maturitas 2004; 47, 71–77.

20.    Barret KE, Barman SM, BOITano S. Ganong review of  medical physiology. 2010; 23th ed.

21.    Monnikhof EM, Velthuis MJ, el. Effect of exercise on postmenopausal sex hormone levels. Journal of clinical oncology 2009; 27: 4402-99

22.    Atkinson C, Lampe JW, et al. Effect of a moderate intensity exercise intervention on estrogen metabolism in postmenopausal woman. 2004; 13: 868-74

23.    Al-Nakkash L, Markus B, Bowden K,Batia LM, Prozialeck WC, Broderick TL. Effectsof acute and 2-day genistein treatment on cardiac function and ischemic tolerance inovariectomized rats. Gender Medicine  2009; 6:488-97.

24.    Al-Nakkash L, Markus B, Batia L, Prozialeck WC, Broderick TL. Genistein induces estrogen-like effects in ovariectomized rats but fails to increase cardiac GLUT4 and oxidative stress. Journal of medicinal food 2010; 13(6):1369-75.

25.    Jaetaek Kim,et a. Insulin-Like Growth Factor I Receptor Signaling Is Required for Exercise-Induced Cardiac Hypertrophy. Molecular Endocrinology 2013; 11:1-11.