The effect of treadmill aerobic training on adipolin, glucose and insulin in type 2 diabetic male rats

Document Type : Research Paper I Open Access I Released under (CC BY-NC 4.0) license

Authors

1 MSc in Exercise Physiology, University of Guilan, Rasht, Iran

2 Assistant Professor in Exercise Physiology, University of Guilan, Rasht, Iran.

Abstract

Background and objective: Secretion of pro-inflammatory and anti-inflammatory cytokines play a critical role in the regulation of metabolic conditions. This study examines the effect of eight weeks aerobic training on Adipolin cytokine and insulin resistance index in type 2 diabetic male rats.
Materials and procedures: Eighteen 8-10 weeks Wister rats with 200-220 gr are selected and they divided equaly into two diabetes [DBT] and control [CON] groups randomly. The training program includes 8 weeks of endurance training on a treadmill at a constant dip of 5%. The speed started from 10 meters per minute and it rised to the 25 meters per minute and its time started from 15 minutes to 45 minutes. In end of 8 weeks training, blood samples were taken after 12 hours of fasting in order to determination of insulin, glucose and Adipulin levels. The homeostatic model assessment [HOMA] was calculated as insulin resistance index. The data were analyzed using the an independent t test.
Findings: Comparisons of two groups showed that a meaningful increase in Adipulin and decrease in glucose and insulin resistance.
Conclusion: According to the findings, it seems that aerobic training with moderate intensity will lead to decreasing in insulin resistance and increase in serum levels of Adipulin in type 2 diabetic rats.

Keywords

Main Subjects

References

 
1. Tan, B.K., et al. (2014). Circulatory changes of the novel adipokine adipolin/CTRP12 in response to metformin treatment and an oral glucose challenge in humans. Clinical endocrinology, 81(6): 841-846.
2. Enomoto, T., et al., (2011). Adipolin/C1qdc2/CTRP12 protein functions as an adipokine that improves glucose metabol ism. Journal of Biological Chemistry, 286(40): 34552-34558.
3. Yang, Q., et al. (2005). Serum retinol binding protein 4 contributes to insulin resistance in obesity and type 2 diabetes. Nature, 436(7049): p. 356-362.
4. Peterson, J.M., Z. Wei, and G.W. Wong. (2010). C1q/TNF-related protein-3 (CTRP3), a novel adipokine that regulates hepatic glucose output. Journal of Biological Chemistry, 285(51): 39691-39701.
5. Frydelund-Larsen, L., et al. (2007). Visfatin mRNA expression in human subcutaneous adipose tissue is regulated by exercise. American Journal of Physiology-Endocrinology and Metabolism, 292(1): p. E24-E31.
6. Tan, B.K., et al. (2013). Metformin increases the novel adipokine adipolin/CTRP12: role of the AMPK pathway. Journal of Endocrinology, 219(2): p. 101-108.
7. Wei, Z., et al. (2012). C1q/TNF-related protein-12 (CTRP12), a novel adipokine that improves insulin sensitivity and glycemic control in mouse models of obesity and diabetes. Journal of Biological Chemistry, 287(13): p. 10301-10315.
8. Shehzad, A., et al. (2012). Adiponectin: regulation of its production and its role in human diseases. Hormones (Athens), 11(1): p. 8-20.
9. Choi, K., et al. (2007). Effect of exercise training on plasma visfatin and eotaxin levels. European Journal of Endocrinology, 157(4): p. 437-442.
10. Arner, P. (2006). Visfatin-a true or false trail to type 2 diabetes mellitus. The Journal of clinical endocrinology and metabolism, 91(1): p. 28.
11. Balducci S, Zanuso S, Nicolucci A, et al. (2010). Anti-inflammatoryeffect of exercise training in subjects with type 2 diabetes and the metabolic syndrome isdependenton exercise modalities and independent of weightloss. Nutr Metab Cardiovasc Dis, 20(8): 608-17.
12. Kawamata Y, Imamura T, Babendure JL, et al. (2007). Tumor necrosis factor receptor-1 can function through a G alphaq/11-betaarrestin- 1 signaling complex. J Biol Chem, 282(39): 28549-56.
13. Ouchi N, Higuchi A, Ohashi K, et al. (2010). Sfrp5 is an anti-inflammatory adipokine that modulates metabolic dysfunction in obesity. Science, 329:454-457.
14. CnopM, FoufelleF, VellosoLA. (2012). Endoplasmicreticulum stress, obesity and diabetes. Trends Mol Med, 18(1): 59-68.
15. Hotamisligil GS, Shargill NS, Spiegelman BM. (1993). Adipose expression of tumor necrosisfactor-alpha: direct role in obesity-linked insulin resistance. Science, 259: 87-91.
16. Henriksen EJ. (2002). Effects of acuteexercise and exercise training on insulin resistance. J Appl Physiol, 93(2): 788-96.
17. Brooks N, Layne JE, Gordon PL, et al. (2007). Strength trainingimproves muscle quality and insulin sensitivity inHispanic older adults with type 2 diabetes. Int JMed Sci, 4(1): 19-27.
18. Rodrigues B, Figueroa D, Mostarda C, et al. (2007). Maxima exercise test is a useful method for physical capacity and oxygen consumption determination in­ streptozotocin-diabetic rats. Cardiovascular Dialectology, 6(38):125-131.
Journal of Applied Health Studies in Sport Physiology
Volume 5, Issue 1
September 2018
Pages 93-105

Files

History

  • Receive Date: 27 January 2019
  • Revise Date: 15 April 2019
  • Accept Date: 02 May 2019

Share

How to cite

Statistics